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Straighterline Micro 250 Quiz 6 Prep Exam
Questions And Answers |Latest 2025 |
Guaranteed Pass
A microbiologist would like to use a noncompetent genus of streptococcal bacteria,
Enterococcus faecalis, as a cloning host to express genes from Streptococcus pneumoniae,
which is naturally competent. Is this possible?
Select one:
a. Yes; electroporation or chemical transformation can be used to make Enterococcus
competent, and then genes from Streptococcus can be introduced via a cloning vector.
b. Yes; electroporation or chemical transformation can be used to make non-competent
Streptococcus mutants, from which genes can be inserted into a cloning vector and introduced
into Enterococcus.
c. No; competence factor is an essential protein that enables the uptake of foreign DNA,
therefore a cloning host such as Enterococcus that lacks competence factor protein is unable to
be transformed by electroporation or chemical transformation.
d. Yes; since the cloning host and the DNA to be introduced are both from bacteria th -
Answer✔Yes; electroporation or chemical transformation can be used to
make Enterococcus competent, and then genes from Streptococcus can be introduced via a
cloning vector.
The three steps that take place in each cycle during PCR occur in which order?
Select one:
a. DNA annealing, denaturation, and synthesis
b. DNA denaturation, annealing, and synthesis
1
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 5:17 PM A+
c. DNA synthesis, denaturation, and annealing
d. None of the choices are correct. - Answer✔DNA denaturation, annealing, and synthesis
In order to express eukaryotic genes in a bacterium, the __________ must first be
removed.
Select one:
a. introns
b. exons
c. enhancers
d. 3' poly A sequence - Answer✔introns
ORF refers to
Select one:
a. the location of the start site of transcription.
b. the region of a gene that codes for a protein or a functional RNA
product.
c. the region of a gene that serves as the promoter.
d. the region between the ribosome binding site and translation initiation - Answer✔the region
of a gene that codes for a protein or a functional
Which of the following is a technology that can be used to identify the DNA to which a
protein binds?
Select one:
a. ChIP-Chip and ChIP-Seq analysis
b. RNA-seq and microarray analysis
c. PCR and RT-PCR analysis
d. Isoelectric focusing and 2D gel electrophoresis analysis - Answer✔ChIP-Chip and ChIP-Seq
analysis
2
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 5:17 PM A+
The oxidized form of inorganic iron that results from microbial oxidative processes is
______.
Select one:
a. ferrous ion
b. ferric ion
c. sulfate ion
d. nitrate ion - Answer✔ferric ion
Less than ______% of all microbial species have been cultured, identified, and
studied.
Select one:
a. 1
b. 2
c. 5
d. 10 - Answer✔1
Which of the following microbes can oxidize ferrous iron while using chlorate or
perchlorate as the electron acceptor?
Select one:
a. Pseudomonas denitrificans
b. Thiobacillus ferrooxidans
c. Ferribacterium limneticum
d. Dechlorosoma suillum - Answer✔Thiobacillus ferrooxidans
The discharge of high levels of microbial nutrients into marine environments has
Select one:
3
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 5:17 PM A+
a. resulted in the production of large hypoxic or anoxic regions.
b. had negative impacts on shellfish and other bottom-dwelling marine animals.
c. resulted in algal blooms and red tides that have killed marine mammals.
d. All of the choices are correct. - Answer✔All of the choices are correct.
The open ocean is a(n) ________ environment.
Select one:
a. eutrophic
b. oligotrophic
c. neotrophic
d. phylotrophic - Answer✔oligotrophic
Which of the following is inconsistent with the current understanding of how plants and
microbes interact with each other?
Select one:
a. Plants are able to send chemical signals to microbes but they are not able to receive signals
from the microbes.
b. Plants produce chemical signaling molecules that microbes can detect and react to.
c. Microbes produce chemical signaling molecules that plants can react to.
d. All of these statements are inconsistent with our understanding of how plants and microbes
interact with each other. - Answer✔Plants are able to send chemical signals to microbes but
they are not able to receive signals from the microbes.
The region of soil in the immediate vicinity of the roots of plants is called the ________.
Select one:
a. plant crust
b. rhizosphere
c. phyllosphere
4
Straighterline Micro 250 Quiz 6 Prep Exam
Questions And Answers |Latest 2025 |
Guaranteed Pass
A microbiologist would like to use a noncompetent genus of streptococcal bacteria,
Enterococcus faecalis, as a cloning host to express genes from Streptococcus pneumoniae,
which is naturally competent. Is this possible?
Select one:
a. Yes; electroporation or chemical transformation can be used to make Enterococcus
competent, and then genes from Streptococcus can be introduced via a cloning vector.
b. Yes; electroporation or chemical transformation can be used to make non-competent
Streptococcus mutants, from which genes can be inserted into a cloning vector and introduced
into Enterococcus.
c. No; competence factor is an essential protein that enables the uptake of foreign DNA,
therefore a cloning host such as Enterococcus that lacks competence factor protein is unable to
be transformed by electroporation or chemical transformation.
d. Yes; since the cloning host and the DNA to be introduced are both from bacteria th -
Answer✔Yes; electroporation or chemical transformation can be used to
make Enterococcus competent, and then genes from Streptococcus can be introduced via a
cloning vector.
The three steps that take place in each cycle during PCR occur in which order?
Select one:
a. DNA annealing, denaturation, and synthesis
b. DNA denaturation, annealing, and synthesis
1
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 5:17 PM A+
c. DNA synthesis, denaturation, and annealing
d. None of the choices are correct. - Answer✔DNA denaturation, annealing, and synthesis
In order to express eukaryotic genes in a bacterium, the __________ must first be
removed.
Select one:
a. introns
b. exons
c. enhancers
d. 3' poly A sequence - Answer✔introns
ORF refers to
Select one:
a. the location of the start site of transcription.
b. the region of a gene that codes for a protein or a functional RNA
product.
c. the region of a gene that serves as the promoter.
d. the region between the ribosome binding site and translation initiation - Answer✔the region
of a gene that codes for a protein or a functional
Which of the following is a technology that can be used to identify the DNA to which a
protein binds?
Select one:
a. ChIP-Chip and ChIP-Seq analysis
b. RNA-seq and microarray analysis
c. PCR and RT-PCR analysis
d. Isoelectric focusing and 2D gel electrophoresis analysis - Answer✔ChIP-Chip and ChIP-Seq
analysis
2
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 5:17 PM A+
The oxidized form of inorganic iron that results from microbial oxidative processes is
______.
Select one:
a. ferrous ion
b. ferric ion
c. sulfate ion
d. nitrate ion - Answer✔ferric ion
Less than ______% of all microbial species have been cultured, identified, and
studied.
Select one:
a. 1
b. 2
c. 5
d. 10 - Answer✔1
Which of the following microbes can oxidize ferrous iron while using chlorate or
perchlorate as the electron acceptor?
Select one:
a. Pseudomonas denitrificans
b. Thiobacillus ferrooxidans
c. Ferribacterium limneticum
d. Dechlorosoma suillum - Answer✔Thiobacillus ferrooxidans
The discharge of high levels of microbial nutrients into marine environments has
Select one:
3
, ©FYNDLAY 2025 ALL RIGHTS RESERVED 5:17 PM A+
a. resulted in the production of large hypoxic or anoxic regions.
b. had negative impacts on shellfish and other bottom-dwelling marine animals.
c. resulted in algal blooms and red tides that have killed marine mammals.
d. All of the choices are correct. - Answer✔All of the choices are correct.
The open ocean is a(n) ________ environment.
Select one:
a. eutrophic
b. oligotrophic
c. neotrophic
d. phylotrophic - Answer✔oligotrophic
Which of the following is inconsistent with the current understanding of how plants and
microbes interact with each other?
Select one:
a. Plants are able to send chemical signals to microbes but they are not able to receive signals
from the microbes.
b. Plants produce chemical signaling molecules that microbes can detect and react to.
c. Microbes produce chemical signaling molecules that plants can react to.
d. All of these statements are inconsistent with our understanding of how plants and microbes
interact with each other. - Answer✔Plants are able to send chemical signals to microbes but
they are not able to receive signals from the microbes.
The region of soil in the immediate vicinity of the roots of plants is called the ________.
Select one:
a. plant crust
b. rhizosphere
c. phyllosphere
4
