OCR A-level biology Microscopes
What are the 4 types of microscopes? - Light, electron , transmission, laser scanning confocal
microscopes.
What makes a good microscope observation drawing? (8) - 1. Title
2. Magnification of image
3. Scale (can be bar or conversion calculation I.e measurement 33mm magnification x400)
4. No shading
5. Clear continuous lines
6. Label lines straight and no heads
7. Label lines do not cross
8. Label lines parallel to the top of the page
What is a light microscopes source of image? - Light
How is the a light microscopes beams focused? - Lenses
What's the maximum effective magnification of a light microscope? - x1500-2000
What's the maximum resolution of a light microscope? - 200nm
Can live specimens be used on light microscopes? - Yes
Can you see a section or surface of the specimen using a light microscope? - Both
Is a light microscope cheap or expensive? - Cheap
Does a light microscope show you Colour or B&W? - Colour
, OCR A-level biology Microscopes
Is staining of the specimen required in light microscopes? - Yes
What is the source of image in a transmission electron microscope? - Electrons
How is the beam focused on a TEM? - Electromagnets
What is the maximum effective magnification on a TEM? - x 1million
What is the maximum resolution on a TEM? - 0.5nm
Can a live specimen be used on a TEM? - No
Does a TEM show a section or surface of the specimen? - Section
Is a TEM cheap or expensive? - Expensive
Are images produced my TEM B&W or Colour? - B&W
Do you have to stain the specimen on a TEM? - Yes, give it a metal coating
What is the source of image on a scanning electron microscope? - Electrons
How is the beam focused on a SEM? - Electromagnets
What is the maximum effective magnification on a SEM? - x 500,000
What is the maximum resolution on an SEM? - 3-10nm