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ETS major field test in biology
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Quiz: Pyrimidine dimers formed in DNA due to UV radiation can be
repaired by?
SOS repair
Double strand break repair
Mismatch repair
Photoreactivation repair (nucleotide excision repair)
Base excision repair
Ans: Photoreactivation repair (nucleotide excision repair)
Quiz: Which of the following repair mechanisms uses methyl groups
to distinguish between parental and daughter strands?
Answers:
Mismatch repair
Photoreactivation repair (Nucleotide excision repair)
SOS repair
Double strand break repair
Sunday, 02 March 2025
,Base excision repair
Ans: Mismatch repair
Quiz: Which of the following types of molecules is always found in
virions?
Answers:
Protein
RNA
Lipid
DNA
Carbohydrate
Ans: Protein
Quiz: A Barr body found within the female mammalian cell's
nucleus is an example of ___
Answers:
Euchromatin
Polytene chromosome
Pseudogenes
Heterochromatin
Cistron
Ans: Heterochromatin
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, Quiz: In vertebrate genes, transcription regulatory regions that
contain CpG islands are inactivated by which CpG modification?
Answers:
Methylation
Ubiquitination
Acetylation
Phosphorylation
Myristoylation
Ans: Methylation
Quiz: TATA-binding protein (TBP) is a key transcription factor in
eukaryotes. A schematic representation of the 240-amino acid TBP of
yeast is shown in Fig. 1 below, with the positions of 2 mutations
indicated. The boxed area represents the evolutionarily conserved C-
terminal domain of TBP, and the shaded (dark) regions of this domain
represent two repeated elements involved in DNA binding. One
mutation (P65S) changes proline 65 to serine and the other I143N
changes isoleucine 143 to asparagine. Fig. 2 below represents
autoradiographs of gels containing RNA transcripts produced in
extracts of wild type or mutant cells. Lanes 1-2 are from wild-type yeast
(WT), lanes 3-4 are from the I143N mutant and lanes 5-6 are from P65S
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, mutant, as indicated. Fig. 2A shows transcripts of a gene transcribed by
RNA polymerase I (Pol I). Fig. 2B shows transcripts of a gene transcribed
by RNA polymerase II (Pol II). Fig. 2C shows transcripts
Ans: Whether mutant transcription phenotypes are due to perturbation of TBP
function.
Quiz: A set of genes from Bacillus subtilis that encode the proteins
required for sporulation have conserved DNA sequences -35 and -10
nucleotides before the site of transcript initiation. However, the
sequence at -35 is different from that seen in most other genes from
that species. Which of the following best explains this difference?
Answers:
Transcription of these genes is induced by cAMP
Translation of the mRNAs transcribed from these genes requires specific
ribosomes that recognize a modified Shine-Dalgarno sequence
A novel sigma factor is required for transcription initiation at these
genes
The -35 sequence is the binding site for a repressor of transcription
The replication of these genes requires a specifically modified DNA
polymerase.
Ans: A novel sigma factor is required for transcription initiation at these
genes
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