D514 Exam Questions and Answers | Verified
What root means tissue?
histo
What root means study?
logy
What is the study of the microscopic anatomy of the tissue?
histology
What microscopic methods are used in histology?
light microscopy
electron microscopy
confocal microscopy
What are the steps in preparation of histological samples?
fixation
embedding
sectioning
staining
What is the preservation of tissue from degradation and maintenance of cellular component
structure?
fixation
What is the most commonly used fixative?
10% formalin
What is the dehydration of tissue with alcohol and placement in paraffin?
embedding
,What is cutting 3-5 um thick tissue sections to be mounted on a glass slide?
sectioning
What is used to cut tissue sections in sectioning?
microtome
What gives contrast to the tissue and highlights particular features of interest?
staining
What is the most commonly used stain in histopathology?
hematoxylin and eosin (H&E)
What stains the nucleus blue?
hematoxylin
Is hematoxylin basophilic or acidophilic?
basophilic
What stains the cytoplasm pink?
eosin
Is eosin basophilic or acidophilic?
acidophilic
What stains polysaccharides, such as glycogen, highlighting the basement membrane and
fungus?
Periodic Acid Schiff (PAS)
What color does trichrome stain mmuscle?
red
What color does trichrome stain collagen?
, blue/green
What color does trichrome stain nuclei?
black
What stain highlights reticular fibers?
silver stain
What selectively stains a specific protein in the tissue using antibodies?
immunohistochemistry
What immunohistochemical antibody stains epithelium?
cytokeratin
What stain is this?
hematoxylin and eosin
What stain is the red arrow pointing to?
eosin
What stain is the purple arrow pointing to?
hematoxylin
What stain is this?
Periodic Acid Schiff
What structure is the red arrow pointing to?
basement membrane
What stain is this?
Periodic Acid Schiff
What is the green arrow pointing to?
What root means tissue?
histo
What root means study?
logy
What is the study of the microscopic anatomy of the tissue?
histology
What microscopic methods are used in histology?
light microscopy
electron microscopy
confocal microscopy
What are the steps in preparation of histological samples?
fixation
embedding
sectioning
staining
What is the preservation of tissue from degradation and maintenance of cellular component
structure?
fixation
What is the most commonly used fixative?
10% formalin
What is the dehydration of tissue with alcohol and placement in paraffin?
embedding
,What is cutting 3-5 um thick tissue sections to be mounted on a glass slide?
sectioning
What is used to cut tissue sections in sectioning?
microtome
What gives contrast to the tissue and highlights particular features of interest?
staining
What is the most commonly used stain in histopathology?
hematoxylin and eosin (H&E)
What stains the nucleus blue?
hematoxylin
Is hematoxylin basophilic or acidophilic?
basophilic
What stains the cytoplasm pink?
eosin
Is eosin basophilic or acidophilic?
acidophilic
What stains polysaccharides, such as glycogen, highlighting the basement membrane and
fungus?
Periodic Acid Schiff (PAS)
What color does trichrome stain mmuscle?
red
What color does trichrome stain collagen?
, blue/green
What color does trichrome stain nuclei?
black
What stain highlights reticular fibers?
silver stain
What selectively stains a specific protein in the tissue using antibodies?
immunohistochemistry
What immunohistochemical antibody stains epithelium?
cytokeratin
What stain is this?
hematoxylin and eosin
What stain is the red arrow pointing to?
eosin
What stain is the purple arrow pointing to?
hematoxylin
What stain is this?
Periodic Acid Schiff
What structure is the red arrow pointing to?
basement membrane
What stain is this?
Periodic Acid Schiff
What is the green arrow pointing to?
