BACE Exam Review UPDATED Questions
and CORRECT Answers
PCR - CORRECT ANSWER - (polymerase chain reaction) multiple copies of a specific
segment of DNA
ELISA - CORRECT ANSWER - enzyme-linked immunosorbent assay, a blood test used to
screen for the presence of antibodies
Western blotting - CORRECT ANSWER - procedure that uses labeled antibodies to detect
specific antigens in a mixture of proteins separated according to their molecular weight
SDS-PAGE - CORRECT ANSWER - Type of Electrophoresis used to separate proteins
based on mass.
Chromatography - CORRECT ANSWER - A technique that is used to separate the
components of a mixture based on the tendency of each component to travel or be drawn across
the surface of another material. (Ion-exchange, Size Exclusion, Affinity, Hydrophobic
Interactions)
Autoclave - CORRECT ANSWER - Piece of equipment used to sterilize articles by way of
steam under pressure and/or dry heat
Fume Hood - CORRECT ANSWER - used to prevent a person from exposure to
hazardous fumes from chemicals
sodium dodecyl sulfate (SDS) - CORRECT ANSWER - Negatively-charged detergent
used to denature and confer a negative charge to proteins before separation by electrophoresis
Cathode - CORRECT ANSWER - Negative electrode
, Anode - CORRECT ANSWER - Positive electrode
spectrophotometer - CORRECT ANSWER - measures a pigment's ability to absorb
various wavelengths and thus giving its concentration when used with a standard curve.
Southern blotting - CORRECT ANSWER - A hybridization technique that enables
researchers to determine the presence of certain nucleotide sequences in a sample of DNA.
Protease - CORRECT ANSWER - enzyme that digests protein
Digest - CORRECT ANSWER - another word meaning: to break down
Northern Blotting - CORRECT ANSWER - A technique that enables specific nucleotide
sequences to be detected in samples of mRNA. It involves gel electrophoresis of RNA molecules
and their transfer to a membrane (blotting), followed by nucleic acid hybridization with a labeled
probe.
nitrocellulose membrane - CORRECT ANSWER - Samples from a gel are transferred to
this before conducting a blotting technique.
Enzymes - CORRECT ANSWER - Proteins that speed up chemical reactions; breaks down
or builds up materials
DNA structure - CORRECT ANSWER - Double Helix Structure; Semi-Conservative
Replication; Contains a nitrogenous base, a phosphate, and a deoxyribose sugar; negatively
charged; Adenine binds with Thymine, Cytosine binds with Guanine.
Transcription - CORRECT ANSWER - Process of creating an mRNA strand from a
sequence of DNA.
and CORRECT Answers
PCR - CORRECT ANSWER - (polymerase chain reaction) multiple copies of a specific
segment of DNA
ELISA - CORRECT ANSWER - enzyme-linked immunosorbent assay, a blood test used to
screen for the presence of antibodies
Western blotting - CORRECT ANSWER - procedure that uses labeled antibodies to detect
specific antigens in a mixture of proteins separated according to their molecular weight
SDS-PAGE - CORRECT ANSWER - Type of Electrophoresis used to separate proteins
based on mass.
Chromatography - CORRECT ANSWER - A technique that is used to separate the
components of a mixture based on the tendency of each component to travel or be drawn across
the surface of another material. (Ion-exchange, Size Exclusion, Affinity, Hydrophobic
Interactions)
Autoclave - CORRECT ANSWER - Piece of equipment used to sterilize articles by way of
steam under pressure and/or dry heat
Fume Hood - CORRECT ANSWER - used to prevent a person from exposure to
hazardous fumes from chemicals
sodium dodecyl sulfate (SDS) - CORRECT ANSWER - Negatively-charged detergent
used to denature and confer a negative charge to proteins before separation by electrophoresis
Cathode - CORRECT ANSWER - Negative electrode
, Anode - CORRECT ANSWER - Positive electrode
spectrophotometer - CORRECT ANSWER - measures a pigment's ability to absorb
various wavelengths and thus giving its concentration when used with a standard curve.
Southern blotting - CORRECT ANSWER - A hybridization technique that enables
researchers to determine the presence of certain nucleotide sequences in a sample of DNA.
Protease - CORRECT ANSWER - enzyme that digests protein
Digest - CORRECT ANSWER - another word meaning: to break down
Northern Blotting - CORRECT ANSWER - A technique that enables specific nucleotide
sequences to be detected in samples of mRNA. It involves gel electrophoresis of RNA molecules
and their transfer to a membrane (blotting), followed by nucleic acid hybridization with a labeled
probe.
nitrocellulose membrane - CORRECT ANSWER - Samples from a gel are transferred to
this before conducting a blotting technique.
Enzymes - CORRECT ANSWER - Proteins that speed up chemical reactions; breaks down
or builds up materials
DNA structure - CORRECT ANSWER - Double Helix Structure; Semi-Conservative
Replication; Contains a nitrogenous base, a phosphate, and a deoxyribose sugar; negatively
charged; Adenine binds with Thymine, Cytosine binds with Guanine.
Transcription - CORRECT ANSWER - Process of creating an mRNA strand from a
sequence of DNA.
