BACE Practical Review UPDATED
ACTUAL Questions and CORRECT
Answers
Restriction Enzyme (Restriction Endonuclease) - CORRECT ANSWER - An enzyme
produced chiefly by certain bacteria, having the property of cleaving/cutting/digesting DNA
molecules at or near a specific sequence of bases.
Spectrophotometer - CORRECT ANSWER - Used to measure protein or DNA
concentration; an apparatus for measuring the intensity of light in a part of the spectrum,
especially as transmitted or emitted by particular substances.
Horizontal Gel Electrophoresis Box - CORRECT ANSWER - Used to run DNA agarose
gels.
Vertical Gel Electrophoresis Box - CORRECT ANSWER - Used to run protein gels
(PAGE or polyacrylamide gel electrophoresis).
Buffer - CORRECT ANSWER - A solvent that is resistant to fluctuations in pH, because it
is made with a combination of weak acid and its conjugate bases.
Acid - CORRECT ANSWER - A chemical substance that neutralizes alkalis, dissolves
some metals, and turns litmus red; typically, a corrosive or sour-tasting liquid of this kind.
Base - CORRECT ANSWER - A substance capable of reacting with an acid to form a salt
and water, or (more broadly) of accepting or neutralizing hydrogen ions.
Genomic DNA Digestion - CORRECT ANSWER - The cleaving of linear DNA; for
example- in genomic DNA when three cuts are made with a restriction enzyme four fragments
result.
, Plasmid DNA Digestion - CORRECT ANSWER - The cleaving of circular DNA; for
example- in plasmid DNA when three cuts are made with a restriction enzyme three fragments
result.
Kilo- - CORRECT ANSWER - 10^3
Ex. 1 kiloliter (kL) = 1000 liters (L)
Milli- - CORRECT ANSWER - 10^-3
Ex. 1 molar (M) = 1000 mM
Micro- - CORRECT ANSWER - 10^-6
Ex. 1 gram (g) = 1,000,000 micrograms (ug)
Loading Dye - CORRECT ANSWER - A solution added to an electrophoresis sample to
give it color and density; 1 microliter is added for every 5 milliliters of sample.
Agarose - CORRECT ANSWER - A polysaccharide, generally extracted from certain red
seaweed, used to make gels for DNA electrophoresis.
pH Scale - CORRECT ANSWER - Measures how acidic or basic a substance is; ranges
from 0 to 14; logarithmic scale- ex. pH 4 to pH 2.. pH 2 is 100 times more acidic.
Significant Figures - CORRECT ANSWER - Each of the digits of a number that are used
to express it to the required degree of accuracy, starting from the first nonzero digit; all non zero
digits are significant; leading zeros (0.002) are not significant; captive zeros (1001) are
significant; trailing zeros (100 or 100.0) are only significant if a decimal is present anywhere
within the measurement.
Spectrophotometry - CORRECT ANSWER - Used to determine the amount, or
concentration, of a protein (or other substance) present in a sample by measuring how much it
absorbs light as it passes through the sample solution.
ACTUAL Questions and CORRECT
Answers
Restriction Enzyme (Restriction Endonuclease) - CORRECT ANSWER - An enzyme
produced chiefly by certain bacteria, having the property of cleaving/cutting/digesting DNA
molecules at or near a specific sequence of bases.
Spectrophotometer - CORRECT ANSWER - Used to measure protein or DNA
concentration; an apparatus for measuring the intensity of light in a part of the spectrum,
especially as transmitted or emitted by particular substances.
Horizontal Gel Electrophoresis Box - CORRECT ANSWER - Used to run DNA agarose
gels.
Vertical Gel Electrophoresis Box - CORRECT ANSWER - Used to run protein gels
(PAGE or polyacrylamide gel electrophoresis).
Buffer - CORRECT ANSWER - A solvent that is resistant to fluctuations in pH, because it
is made with a combination of weak acid and its conjugate bases.
Acid - CORRECT ANSWER - A chemical substance that neutralizes alkalis, dissolves
some metals, and turns litmus red; typically, a corrosive or sour-tasting liquid of this kind.
Base - CORRECT ANSWER - A substance capable of reacting with an acid to form a salt
and water, or (more broadly) of accepting or neutralizing hydrogen ions.
Genomic DNA Digestion - CORRECT ANSWER - The cleaving of linear DNA; for
example- in genomic DNA when three cuts are made with a restriction enzyme four fragments
result.
, Plasmid DNA Digestion - CORRECT ANSWER - The cleaving of circular DNA; for
example- in plasmid DNA when three cuts are made with a restriction enzyme three fragments
result.
Kilo- - CORRECT ANSWER - 10^3
Ex. 1 kiloliter (kL) = 1000 liters (L)
Milli- - CORRECT ANSWER - 10^-3
Ex. 1 molar (M) = 1000 mM
Micro- - CORRECT ANSWER - 10^-6
Ex. 1 gram (g) = 1,000,000 micrograms (ug)
Loading Dye - CORRECT ANSWER - A solution added to an electrophoresis sample to
give it color and density; 1 microliter is added for every 5 milliliters of sample.
Agarose - CORRECT ANSWER - A polysaccharide, generally extracted from certain red
seaweed, used to make gels for DNA electrophoresis.
pH Scale - CORRECT ANSWER - Measures how acidic or basic a substance is; ranges
from 0 to 14; logarithmic scale- ex. pH 4 to pH 2.. pH 2 is 100 times more acidic.
Significant Figures - CORRECT ANSWER - Each of the digits of a number that are used
to express it to the required degree of accuracy, starting from the first nonzero digit; all non zero
digits are significant; leading zeros (0.002) are not significant; captive zeros (1001) are
significant; trailing zeros (100 or 100.0) are only significant if a decimal is present anywhere
within the measurement.
Spectrophotometry - CORRECT ANSWER - Used to determine the amount, or
concentration, of a protein (or other substance) present in a sample by measuring how much it
absorbs light as it passes through the sample solution.
