BACE Qualifier UPDATED ACTUAL
Questions and CORRECT Answers
What is a disadvantage to using prokaryotes in the production of biopharmaceuticals? -
CORRECT ANSWER - Prokaryotes are unable to properly fold and assemble more
complicated eukaryotic proteins.
Which structure regulates the flow of molecules into and out of the cell? - CORRECT
ANSWER - Plasma membrane
Why is E. coli usually incubated at 37°C? - CORRECT ANSWER - The maximum growth
rate for E. coli occurs at 37°C
Which of the following serves as the decolorizer in a Gram stain? - CORRECT
ANSWER - Alcohol
Which of the following is an accurate description of Gram positive bacteria after Gram staining?
- CORRECT ANSWER - Purple, because gram positive bacteria have a thick layer of
peptidoglycan in the cell membrane.
When exposed to a foreign antigen, the normal human immune system response is to produce
which of the following? - CORRECT ANSWER - Antibodies
Why are biopharmaceuticals typically more difficult to manufacture than traditional chemically-
synthesized pharmaceuticals? - CORRECT ANSWER - Biopharmaceuticals are more
prone to contamination.
Biopharmaceuticals cannot be heat sterilized like traditional pharmaceuticals.
Biopharmaceuticals typically require special handling and storage.
ALL OF THE ABOVE
,Which of the following features of bacterial plasmids can be used as a marker when growing
bacterial cultures? - CORRECT ANSWER - Antibiotic resistance gene
The observable traits of an organism are referred to as the: - CORRECT ANSWER -
Phenotype
According to the Central Dogma of molecular biology, DNA is __________ into RNA, which is
___________ into protein. - CORRECT ANSWER - transcribed, translated
Flame your loop and let it cool/aseptically open a new disposable loop - CORRECT
ANSWER - Step 1
Open the lid of the Petri plate just enough to insert the loop - CORRECT ANSWER - Step
2
Streak across quadrant 1 and into quadrant 2 - CORRECT ANSWER - Step 3
Streak back and forth to cover quadrant 2 - CORRECT ANSWER - Step 4
When preparing agar plates for bacterial culture, which of the following is the best next step after
streaking the plates with bacteria? - CORRECT ANSWER - Confirm incubator
temperature is correct, then place plates upside-down inside the incubator
What is the purpose of using the quadrant method when streaking bacteria on agar plates? -
CORRECT ANSWER - To dilute out individual bacterial cells leading to isolated colonies
Mixes or homogenizes small volume solutions - CORRECT ANSWER - Vortexer
Enclosed, ventilated lab workspace - CORRECT ANSWER - biosafety cabinet
, Separates molecules based on size and charge - CORRECT ANSWER - Electrophoresis
System
Measures acidity and alkalinity of solutions - CORRECT ANSWER - pH Meter
Mixes cell cultures at a constant temperature - CORRECT ANSWER - Shaking Incubator
Sterilizes contents with high pressure and steam - CORRECT ANSWER - Autoclave
Amplifies DNA by heating and cooling - CORRECT ANSWER - Thermal Cycler
Measures mass - CORRECT ANSWER - balance
Measures and delivers volumes under 1.0 mL - CORRECT ANSWER - micropipet
Separates substances based on density - CORRECT ANSWER - centrifuge
What is this? - CORRECT ANSWER - Erlenmeyer Flask
What is this? - CORRECT ANSWER - micropipet
What is this? - CORRECT ANSWER - media bottle
What is this? - CORRECT ANSWER - Thermal Cycler
What is this? - CORRECT ANSWER - graduated cylinder
Questions and CORRECT Answers
What is a disadvantage to using prokaryotes in the production of biopharmaceuticals? -
CORRECT ANSWER - Prokaryotes are unable to properly fold and assemble more
complicated eukaryotic proteins.
Which structure regulates the flow of molecules into and out of the cell? - CORRECT
ANSWER - Plasma membrane
Why is E. coli usually incubated at 37°C? - CORRECT ANSWER - The maximum growth
rate for E. coli occurs at 37°C
Which of the following serves as the decolorizer in a Gram stain? - CORRECT
ANSWER - Alcohol
Which of the following is an accurate description of Gram positive bacteria after Gram staining?
- CORRECT ANSWER - Purple, because gram positive bacteria have a thick layer of
peptidoglycan in the cell membrane.
When exposed to a foreign antigen, the normal human immune system response is to produce
which of the following? - CORRECT ANSWER - Antibodies
Why are biopharmaceuticals typically more difficult to manufacture than traditional chemically-
synthesized pharmaceuticals? - CORRECT ANSWER - Biopharmaceuticals are more
prone to contamination.
Biopharmaceuticals cannot be heat sterilized like traditional pharmaceuticals.
Biopharmaceuticals typically require special handling and storage.
ALL OF THE ABOVE
,Which of the following features of bacterial plasmids can be used as a marker when growing
bacterial cultures? - CORRECT ANSWER - Antibiotic resistance gene
The observable traits of an organism are referred to as the: - CORRECT ANSWER -
Phenotype
According to the Central Dogma of molecular biology, DNA is __________ into RNA, which is
___________ into protein. - CORRECT ANSWER - transcribed, translated
Flame your loop and let it cool/aseptically open a new disposable loop - CORRECT
ANSWER - Step 1
Open the lid of the Petri plate just enough to insert the loop - CORRECT ANSWER - Step
2
Streak across quadrant 1 and into quadrant 2 - CORRECT ANSWER - Step 3
Streak back and forth to cover quadrant 2 - CORRECT ANSWER - Step 4
When preparing agar plates for bacterial culture, which of the following is the best next step after
streaking the plates with bacteria? - CORRECT ANSWER - Confirm incubator
temperature is correct, then place plates upside-down inside the incubator
What is the purpose of using the quadrant method when streaking bacteria on agar plates? -
CORRECT ANSWER - To dilute out individual bacterial cells leading to isolated colonies
Mixes or homogenizes small volume solutions - CORRECT ANSWER - Vortexer
Enclosed, ventilated lab workspace - CORRECT ANSWER - biosafety cabinet
, Separates molecules based on size and charge - CORRECT ANSWER - Electrophoresis
System
Measures acidity and alkalinity of solutions - CORRECT ANSWER - pH Meter
Mixes cell cultures at a constant temperature - CORRECT ANSWER - Shaking Incubator
Sterilizes contents with high pressure and steam - CORRECT ANSWER - Autoclave
Amplifies DNA by heating and cooling - CORRECT ANSWER - Thermal Cycler
Measures mass - CORRECT ANSWER - balance
Measures and delivers volumes under 1.0 mL - CORRECT ANSWER - micropipet
Separates substances based on density - CORRECT ANSWER - centrifuge
What is this? - CORRECT ANSWER - Erlenmeyer Flask
What is this? - CORRECT ANSWER - micropipet
What is this? - CORRECT ANSWER - media bottle
What is this? - CORRECT ANSWER - Thermal Cycler
What is this? - CORRECT ANSWER - graduated cylinder
