D514 - Intro to Histology & Epithelium|
Questions with Complete Solutions
Histology
Studies the microscopic anatomy of the tissue
Methods of Histology
Light microscopy, electron microscopy & confocal microscopy
What are the steps of preparation of histological samples?
1. Fixation
2. Embedding
3. Sectioning
4. Staining
What is the fixation step of repairing a histological sample?
Preserving tissue from degradation and to maintain the structure of cellular
components; most commonly used fixative is 10% formalin
What is the most commonly used fixative in histological samples?
10% formalin
What is the embedding step of repairing a histological sample?
Tissue is dehydrated with alcohol and embedded in paraffin
What is the sectioning step of repairing a histological sample?
A microtome is used to cut 3-5 um thick tissue sections which are later mounted on a
glass slide
, What is the staining step of repairing a histological sample?
To give contrast to the tissue and highlight particular features of interest
What is the most common histological stains?
Hematoxylin and eosin (H&E)
What are the special stains used in histology?
periodic acid schiff (PAS), trichromatic, silver stain and immunohistochemistry stain
What is hematoxylin and eosin (H&E)?
Most commonly used stain in histopathology, hematoxylin (basopilic) and eosin
(acidophilic)
What color does hematoxylin stain?
Stains the nucleus blue
What color does eosin stain?
Stains cytoplasm pink
What does periodic acid Schiff (PAS) stain?
Stains the polysaccharides such as glycogen and highlights the basement membrane,
fungus
What does trichrome stain?
Stains muscle red, collagen blue/green and the nuclei black
What does silver stain stains?
Highlights the reticular fibers in connective tissues
What does the immunohistochhemistry stain?
Questions with Complete Solutions
Histology
Studies the microscopic anatomy of the tissue
Methods of Histology
Light microscopy, electron microscopy & confocal microscopy
What are the steps of preparation of histological samples?
1. Fixation
2. Embedding
3. Sectioning
4. Staining
What is the fixation step of repairing a histological sample?
Preserving tissue from degradation and to maintain the structure of cellular
components; most commonly used fixative is 10% formalin
What is the most commonly used fixative in histological samples?
10% formalin
What is the embedding step of repairing a histological sample?
Tissue is dehydrated with alcohol and embedded in paraffin
What is the sectioning step of repairing a histological sample?
A microtome is used to cut 3-5 um thick tissue sections which are later mounted on a
glass slide
, What is the staining step of repairing a histological sample?
To give contrast to the tissue and highlight particular features of interest
What is the most common histological stains?
Hematoxylin and eosin (H&E)
What are the special stains used in histology?
periodic acid schiff (PAS), trichromatic, silver stain and immunohistochemistry stain
What is hematoxylin and eosin (H&E)?
Most commonly used stain in histopathology, hematoxylin (basopilic) and eosin
(acidophilic)
What color does hematoxylin stain?
Stains the nucleus blue
What color does eosin stain?
Stains cytoplasm pink
What does periodic acid Schiff (PAS) stain?
Stains the polysaccharides such as glycogen and highlights the basement membrane,
fungus
What does trichrome stain?
Stains muscle red, collagen blue/green and the nuclei black
What does silver stain stains?
Highlights the reticular fibers in connective tissues
What does the immunohistochhemistry stain?
