MCB2010 - EXAM 2 QUESTIONS AND ANSWERS
Order the following from most to least resistant in the environment:
gram positive
gram negative
endospores - Answers - endospores
gram negative
gram positive
sterilization - Answers - destruction or removal of all viable organisms from an object or
from a particular environment
disinfection - Answers - killing, inhibition, or removal of pathogenic microorganisms
(usually on inanimate objects)
antisepsis - Answers - prevention of infection of living tissue by microorganisms
sanitization - Answers - reduction of the microbial population to a safe level as determined
by public health standards
What is the difference between a bactericidal and a bacteriostatic agent? - Answers - cidal
- kills
static - inhibits
Differentiate between physical and chemical modes of controlling microorganisms. -
Answers - Physical
Heat
Low temperatures
Filtration
Radiation
Chemical
High-level germicides
Intermediate-level disinfectants and antiseptics
Low-level disinfectants
Comparing dry and moist heat, which is faster and penetrates better? - Answers - moist
What is the chamber that uses steam under pressure? What are its main uses? - Answers
- autoclave; kill bacteria, spores and germs resistant to boiling water and powerful
detergents i.e. lab equipment, tattooing, hospitals
What is the function of pasteurization? Give examples for use. - Answers - doesn't
denatures proteins kills bacteria
Used for milk, ice cream, yogurt, and fruit juices
Order the following from most to least resistant in the environment:
gram positive
gram negative
endospores - Answers - endospores
gram negative
gram positive
sterilization - Answers - destruction or removal of all viable organisms from an object or
from a particular environment
disinfection - Answers - killing, inhibition, or removal of pathogenic microorganisms
(usually on inanimate objects)
antisepsis - Answers - prevention of infection of living tissue by microorganisms
sanitization - Answers - reduction of the microbial population to a safe level as determined
by public health standards
What is the difference between a bactericidal and a bacteriostatic agent? - Answers - cidal
- kills
static - inhibits
Differentiate between physical and chemical modes of controlling microorganisms. -
Answers - Physical
Heat
Low temperatures
Filtration
Radiation
Chemical
High-level germicides
Intermediate-level disinfectants and antiseptics
Low-level disinfectants
Comparing dry and moist heat, which is faster and penetrates better? - Answers - moist
What is the chamber that uses steam under pressure? What are its main uses? - Answers
- autoclave; kill bacteria, spores and germs resistant to boiling water and powerful
detergents i.e. lab equipment, tattooing, hospitals
What is the function of pasteurization? Give examples for use. - Answers - doesn't
denatures proteins kills bacteria
Used for milk, ice cream, yogurt, and fruit juices
