Serology Final Exam || with Right Answers.
Indirect immunofluorescence is similar to which type of enzyme immunoassay in terms of the
performance of the assay?
-capture
-competitive
-ELISA
-homogenous correct answers ELISA
a serological test that uses RBCs coated with exogenous antigens such as bacterial
polysaccharides as a method to detect patients antibodies against that exogenous antigen is
called:
-hemagglutination
-latex agglutination
-neutralization
-complement fixation correct answers hemagglutination
The following pattern of agglutination was observed in an antibody titration
Tube 1: 1 2 3 4 5 6 7 8 9 10 11
1+ 2+ 4+ 4+ 3+ 3+ 2+ 1+ 1+ 0 0
this set of reactions most likely resulted from:
-prozoning
-faulty pipetting technique
-postzoning
-the presence of a high titer, low avidity antibody correct answers prozoning
which statement about noncompetitive enzyme immunoassays is true?
-all patient antigen is allowed to react with binding sites
-binding sites for patient antigen are limited
-washing in between steps is not necessary
-color is indirectly proportional to the concentration correct answers all patient antigen is
allowed to react with binding sites
the definition of titer is the correct answers reciprocal of the last positive dilution
which of the following describes an antigen-antibody precipitation reaction of non identity?
-precipitin line fuss, forming a single spur
-no precipitin lines are formed
-precipitin lines fuse, forming a single arc
-precipitin lines fuse, forming a double spur correct answers -precipitin lines fuse, forming a
double spur
Which test has the greatest sensitivity for antigen detection? correct answers enzyme linked
immunosorbent assay-ELISA
, An immunoassay is performed in the following way: antigen is bound to a solid support. Patient
serum is added and patient antibodies bind to the antigen. The well is washed, and enzyme-
labeled anti-immunoglobulin antibodies are added. The well is washed, and enzyme substrate is
added. Enzyme is determined. Which immunoassay is described? correct answers ELISA
which immunoglobulin appears int he highest titer in the secondary response? correct answers
IgG
The first labeled assays developed were correct answers radioimmunoassay
A patient has been exposed to an inhaled fungus, and it seems likely that she has a beginning
infection. The current approved test for diagnosis is an Outerlony test with her antibody sample
in a well and several different fungal antigens in wells surrounding it. No precipitin line occurred
correct answers the patient's blood should be drawn again in 2 weeks and retested
A soluble antigen and soluble antibody reacting to form an insoluble product describes correct
answers precipitation reactions
The strength of a visible reaction is known as: correct answers avidity
which region of the immunoglobulin molecule can bind antigen? correct answers Fab
which antibody class may require the use of an enhancement technique to visualize the reaction?
correct answers IgG
all of the following are desirable characteristics of antibodies used in immunoassays except
correct answers high cross reactivity
What is the best procedure for removing gloves when leaving the laboratory? correct answers
take gloves off and wash hands
When we ran the serum of patient who seems to be severely ill with arthritis at the lowest
dilution suggested by the latex agglutination kit, the results were negative. What should we do?
correct answers dilute the sample and run it again before the results are reported
Which class of immunoglobulin possesses 10 antigenic binding sites? correct answers IgM
The probability that a person with a positive screening test actually has the disease screened for
is called: correct answers positive predictive value
In a capture assay to determine the presence of a viral antigen in a patient sample, which of the
following would be on solid phase? correct answers unlabeled antibody
what is the most appropriate laboratory safety wear? correct answers fluid-resistant laboratory
gown with wrists cuffs
Indirect immunofluorescence is similar to which type of enzyme immunoassay in terms of the
performance of the assay?
-capture
-competitive
-ELISA
-homogenous correct answers ELISA
a serological test that uses RBCs coated with exogenous antigens such as bacterial
polysaccharides as a method to detect patients antibodies against that exogenous antigen is
called:
-hemagglutination
-latex agglutination
-neutralization
-complement fixation correct answers hemagglutination
The following pattern of agglutination was observed in an antibody titration
Tube 1: 1 2 3 4 5 6 7 8 9 10 11
1+ 2+ 4+ 4+ 3+ 3+ 2+ 1+ 1+ 0 0
this set of reactions most likely resulted from:
-prozoning
-faulty pipetting technique
-postzoning
-the presence of a high titer, low avidity antibody correct answers prozoning
which statement about noncompetitive enzyme immunoassays is true?
-all patient antigen is allowed to react with binding sites
-binding sites for patient antigen are limited
-washing in between steps is not necessary
-color is indirectly proportional to the concentration correct answers all patient antigen is
allowed to react with binding sites
the definition of titer is the correct answers reciprocal of the last positive dilution
which of the following describes an antigen-antibody precipitation reaction of non identity?
-precipitin line fuss, forming a single spur
-no precipitin lines are formed
-precipitin lines fuse, forming a single arc
-precipitin lines fuse, forming a double spur correct answers -precipitin lines fuse, forming a
double spur
Which test has the greatest sensitivity for antigen detection? correct answers enzyme linked
immunosorbent assay-ELISA
, An immunoassay is performed in the following way: antigen is bound to a solid support. Patient
serum is added and patient antibodies bind to the antigen. The well is washed, and enzyme-
labeled anti-immunoglobulin antibodies are added. The well is washed, and enzyme substrate is
added. Enzyme is determined. Which immunoassay is described? correct answers ELISA
which immunoglobulin appears int he highest titer in the secondary response? correct answers
IgG
The first labeled assays developed were correct answers radioimmunoassay
A patient has been exposed to an inhaled fungus, and it seems likely that she has a beginning
infection. The current approved test for diagnosis is an Outerlony test with her antibody sample
in a well and several different fungal antigens in wells surrounding it. No precipitin line occurred
correct answers the patient's blood should be drawn again in 2 weeks and retested
A soluble antigen and soluble antibody reacting to form an insoluble product describes correct
answers precipitation reactions
The strength of a visible reaction is known as: correct answers avidity
which region of the immunoglobulin molecule can bind antigen? correct answers Fab
which antibody class may require the use of an enhancement technique to visualize the reaction?
correct answers IgG
all of the following are desirable characteristics of antibodies used in immunoassays except
correct answers high cross reactivity
What is the best procedure for removing gloves when leaving the laboratory? correct answers
take gloves off and wash hands
When we ran the serum of patient who seems to be severely ill with arthritis at the lowest
dilution suggested by the latex agglutination kit, the results were negative. What should we do?
correct answers dilute the sample and run it again before the results are reported
Which class of immunoglobulin possesses 10 antigenic binding sites? correct answers IgM
The probability that a person with a positive screening test actually has the disease screened for
is called: correct answers positive predictive value
In a capture assay to determine the presence of a viral antigen in a patient sample, which of the
following would be on solid phase? correct answers unlabeled antibody
what is the most appropriate laboratory safety wear? correct answers fluid-resistant laboratory
gown with wrists cuffs
