Microbiology Lab Midterm Exam 2025 Questions & Answers with 100% Correct Answers | Verified

Compound microscope - 1. Ocular-eyepiece lens (usually 10x) 2. Objective-nosepiece lenses (commonly 4x, 10x, 45x, and 100x) At what magnification do you use the oil immersion technique? - 100x. Total Magnification = - Magnification (Objective) x Magnification (Ocular) Important Parts of the Microscope - Framework, Stage, and Lens System Framework - Arm and base are the structural parts of the microscope which support the basic frame. Stage - Holds the slide. The mechanical stage clamps the slide and moves the slide around the stage. Lens System - Oculars, Objective, and Condenser Oculars - Eyepiece lenses (usually 10x magnification) Objectives - Lenses attached to rotatable nosepiece, common magnification of 4x, 10x, 45x and 100x. Parfocalized microscope focusing adjustments are not to be made when... - changing the objective lenses. The oil has approximately the same refractive index as glass to prevent... - light loss due to diffraction (bending of light rays) which would occur if light traveled from one refractive index to another (eg. glass to air)