microbiology lab final Exam 2025 Questions & Answers with 100% Correct Answers | Verified

Which staining technique is used to determine the thickness of the peptidoglycan layer based on the cell wall composition? - Gram stain Where would you properly dispose of the following items? - Contaminated slides: Slide trays on student benches Pipettes: Nalgene buckets on student benches Used test tubes: Trash cart at front of lab Uncontaminated broken glass: Broken glass box at front of lab (and notify the TA!) When are open toed-shoes and shorts allowed in the microbiology labs? - Never What do you use to clean the ocular lenses and objective lenses on your microscope? - Lens cleaner and lens paper ________________________ microscopy is used to view dead, stained microorganisms, and ________________________ microscopy is used to view live, unstained microorganisms - Brightfield, phase-contrast What is the purpose of isolation streaks on your environmental isolate? - To purify one species of bacteria In microscopy, what are the three main factors to obtain a good image? - Magnification, resolution, and contrast Which objective lens is used with immersion oil? - 100X objective lens When you first enter the lab and again before you leave the lab, what are two things that you should always do? - Wash your hands and wipe down the lab bench with QT solution