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Microbiology Lab Final Exam 2025 Questions & Answers with 100% Correct Answers | Verified

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Give the steps of a PCR - Heat reaction to 95 C to denature DNA Cool rxn to 55 C to allow primers to anneal Heat rxn to 72 C to allow Taq polymerase to bind What machine is used to carry out a PCR reaction? - thermocycler How many cycles does a PCR rxn usually go? - 30 Name the 9 organisms used in the unknown lab - Bacillus subtillis Enterobacter aerogenes Entero cloacae Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa Salmonella typhimurium Staphylococcus aureus Staphylococcus epidermidis Which organisms can ferment lactose? - E. coli E. cloacae E. aerogenes K. pneumonia Define caliform bacteria - rod-shaped, Gram-negative, non-spore forming, and motile or non-motile bacteria which can ferment lactose with the production of acid and gas

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