Microbiology Lab Exam 1 Note Cards 2025 Questions & Answers with 100% Correct Answers | Verified

when viewing a smear with the oil immersion lens the final magnification is - 1000x;10x ocular x 100X objective what is the purpose of heat fixing - kill the bacteria, adhere to glass anddenature the proteins of bacteria name oneof the stains or regents used in the gram stain procedure - gram crystal violet, gram iodine, gram sfaranin, 95% ethanol why are aseptic techniques used in the lab? - to ensure that we do not contaminate what we are working with as as to ensue that we do not acciently inoculate ouselves. example of aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming the test tube before and after obtaining the sample. the gram reaction is due to differences in the structure of wat part of the microbe - its cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer compared to GN bacteria. Name 2 inoculating tools you will be using in this lab - loop, needle what color is a gram positive microbe - purple waht is the function of the ethanol in the gram stain procedure - decolorize name one of the strains in the acid fast stain - cold kinyoun, carbol fuschsin, methylen blue name one of the stains in the endospore stain - malachite green, safranin