Microbiology Lab – Stains Exam 2025 Questions & Answers with 100% Correct Answers | Verified

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Microbiology Lab – Stains Exam 2025 Questions & Answers with 100% Correct Answers | Verified

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2024/2025

Institution Microbiology

Course Microbiology

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Simple stain - -Add color to cells for viewing -Highlight entire organism's shape and basic structures -Includes: Methylene blue Safranin Crystal violet Carbolfuchsin Negative stain - -Enhances viewing of morphology, capsules -Bacteria are live -No heat fixing! (no denaturing) -Nigrosin ink -View under oil -Anionic (acidic) simple stain Gram stain - -Determines cell wall type (gram positive or negative) -Differential stain Gram stain procedure - 1. Crystal violet 2. Iodine (mordant) 3. Alcohol (decolorizer) 4. Safranin (counterstain) 5. Observe under oil Mordant - gram stain - -Increases intensity of crystal violet -Iodine enters cell forms crystal violet-iodine complex (CV-I)

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Uploaded on: February 4, 2025
Number of pages: 3
Written in: 2024/2025
Type: Exam (elaborations)
Contains: Questions & answers

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