MOLECULAR TECHNIQUES- LECTURE 4 REVIEW
TEST AND STUDY GUIDE QUESTIONS AND
ACCURATE VERIFIED ANSWERS GRADED A+
What are the 4 hypotheses that explain the ability of enhancers
to act at a distance -Correct Answer-
1. Change in topology
2. Sliding
3. Looping
4. Facilitated tracking
-have more than one activator-binding site permitting them to
respond to multiple stimuli
-activators that bind at these sites must be able to interact with
the preinitiation complex assembling at the promoter, likely by
looping out any intervening DNA -Correct Answer-complex
enhancers
What are the 2 ways that insulators act by -Correct Answer-
1. enhancer blocking activity
2. barrier activity
insulator between promoter and enhancer prevents the promoter
from being activated -Correct Answer-enhancer blocking
insulator between promoter and condensed, repressive
chromatin prevents promoter from being repressed -Correct
Answer-barrier activity
, -Activator bound to an enhancer and stimulator slides along
DNA from enhancer to promoter -Correct Answer-sliding model
-Two insulators flank an enhancer, when bound they interact
with each other isolating enhancer -Correct Answer-looping
model
What are the 3 phases of transcription? -Correct Answer-
1. Initiation
2. Elongation
3. Termination
True or False:
There is only one RNA polymerase operating in eukaryotic
nuclei -Correct Answer-False:
There are at least 2 RNA polymerases
-One transcribes major ribosomal RNA genes
-One or more to transcribe rest of nuclear genes -Correct
Answer-2 RNA polymerases operating in eukaryotes
What makes Ribosomal genes are different from other nuclear
genes? -Correct Answer--Different base composition from other
nuclear genes
-Unusually repetitive
-Found in different compartment, the nucleolus
-found in nucleolus
-Location suggests in transcribes rRNA genes -Correct Answer-
RNA Polymerase I
TEST AND STUDY GUIDE QUESTIONS AND
ACCURATE VERIFIED ANSWERS GRADED A+
What are the 4 hypotheses that explain the ability of enhancers
to act at a distance -Correct Answer-
1. Change in topology
2. Sliding
3. Looping
4. Facilitated tracking
-have more than one activator-binding site permitting them to
respond to multiple stimuli
-activators that bind at these sites must be able to interact with
the preinitiation complex assembling at the promoter, likely by
looping out any intervening DNA -Correct Answer-complex
enhancers
What are the 2 ways that insulators act by -Correct Answer-
1. enhancer blocking activity
2. barrier activity
insulator between promoter and enhancer prevents the promoter
from being activated -Correct Answer-enhancer blocking
insulator between promoter and condensed, repressive
chromatin prevents promoter from being repressed -Correct
Answer-barrier activity
, -Activator bound to an enhancer and stimulator slides along
DNA from enhancer to promoter -Correct Answer-sliding model
-Two insulators flank an enhancer, when bound they interact
with each other isolating enhancer -Correct Answer-looping
model
What are the 3 phases of transcription? -Correct Answer-
1. Initiation
2. Elongation
3. Termination
True or False:
There is only one RNA polymerase operating in eukaryotic
nuclei -Correct Answer-False:
There are at least 2 RNA polymerases
-One transcribes major ribosomal RNA genes
-One or more to transcribe rest of nuclear genes -Correct
Answer-2 RNA polymerases operating in eukaryotes
What makes Ribosomal genes are different from other nuclear
genes? -Correct Answer--Different base composition from other
nuclear genes
-Unusually repetitive
-Found in different compartment, the nucleolus
-found in nucleolus
-Location suggests in transcribes rRNA genes -Correct Answer-
RNA Polymerase I
