MB (ASCP) Exam UPDATED Questions
and CORRECT Answers
Predominant form of DNA - CORRECT ANSWER - Right-handed B
DNA and histone interaction is due to what type of bond? - CORRECT ANSWER - Ionic
What stains to the minor groove of DNA duplex? - CORRECT ANSWER - SYBR Green
What is the most abundant type of RNA? - CORRECT ANSWER - Ribosomal RNA
(rRNA)
What does RNA act as in a retrovirus? - CORRECT ANSWER - Genome and mRNA
What increases half-life of mRNA? - CORRECT ANSWER - 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - CORRECT ANSWER -
Euchromatin
What is the most common type of eukaryotic DNA? - CORRECT ANSWER - Satellite
DNA
What specimen has the most DNA according to molecular weight? - CORRECT
ANSWER - White Blood Cells
What amino acids compose histones? - CORRECT ANSWER - Lysine and Arginine
Amino acid that all proteins start with: - CORRECT ANSWER - Methionine
,What protects the DNA strand during replication? - CORRECT ANSWER - Single-
stranded binding protein
Leading Strand - CORRECT ANSWER - 3' to 5' template
Lagging Strand - CORRECT ANSWER - 5' to 3' template
What DNA strand is being copied in transcription? - CORRECT ANSWER - Antisense
Strand
First step after newly formed mRNA is made? - CORRECT ANSWER - Addition of
poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
CORRECT ANSWER - Telomerase
What polymerase has repair activity? - CORRECT ANSWER - Beta/Epsilon
What polymerase acts on leading strand? - CORRECT ANSWER - Delta
What polymerase acts on lagging strand? - CORRECT ANSWER - Alpha
Which polymerase is used for mitochondrial replication and repair? - CORRECT
ANSWER - Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - CORRECT ANSWER -
Topoisomerase
, Which RNA polymerase is mainly for transcription? - CORRECT ANSWER - RNA Pol II
Which polymerase is involved with mRNA synthesis? - CORRECT ANSWER - RNA Pol
II
What inactivates RNAses? - CORRECT ANSWER - Diethyl pyrocarbonate (DEPC) or
bake glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - CORRECT ANSWER -
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - CORRECT ANSWER -
Restriction Enzyme Type II
What is necessary to make cDNA? - CORRECT ANSWER - Reverse transcriptase
Number of SNPs in a human genome? - CORRECT ANSWER - 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - CORRECT ANSWER -3
billion base pairs
The polymerization of acrylamide requires: - CORRECT ANSWER - Catalyst (APS or
TEMED) or Light activation
How does agarose gel pore size relate to concentration? - CORRECT ANSWER -
Inversely proportional
What is denatured during a southern blot? - CORRECT ANSWER - Hydrogen bonds
(inter-strand bonds)
and CORRECT Answers
Predominant form of DNA - CORRECT ANSWER - Right-handed B
DNA and histone interaction is due to what type of bond? - CORRECT ANSWER - Ionic
What stains to the minor groove of DNA duplex? - CORRECT ANSWER - SYBR Green
What is the most abundant type of RNA? - CORRECT ANSWER - Ribosomal RNA
(rRNA)
What does RNA act as in a retrovirus? - CORRECT ANSWER - Genome and mRNA
What increases half-life of mRNA? - CORRECT ANSWER - 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - CORRECT ANSWER -
Euchromatin
What is the most common type of eukaryotic DNA? - CORRECT ANSWER - Satellite
DNA
What specimen has the most DNA according to molecular weight? - CORRECT
ANSWER - White Blood Cells
What amino acids compose histones? - CORRECT ANSWER - Lysine and Arginine
Amino acid that all proteins start with: - CORRECT ANSWER - Methionine
,What protects the DNA strand during replication? - CORRECT ANSWER - Single-
stranded binding protein
Leading Strand - CORRECT ANSWER - 3' to 5' template
Lagging Strand - CORRECT ANSWER - 5' to 3' template
What DNA strand is being copied in transcription? - CORRECT ANSWER - Antisense
Strand
First step after newly formed mRNA is made? - CORRECT ANSWER - Addition of
poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
CORRECT ANSWER - Telomerase
What polymerase has repair activity? - CORRECT ANSWER - Beta/Epsilon
What polymerase acts on leading strand? - CORRECT ANSWER - Delta
What polymerase acts on lagging strand? - CORRECT ANSWER - Alpha
Which polymerase is used for mitochondrial replication and repair? - CORRECT
ANSWER - Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - CORRECT ANSWER -
Topoisomerase
, Which RNA polymerase is mainly for transcription? - CORRECT ANSWER - RNA Pol II
Which polymerase is involved with mRNA synthesis? - CORRECT ANSWER - RNA Pol
II
What inactivates RNAses? - CORRECT ANSWER - Diethyl pyrocarbonate (DEPC) or
bake glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - CORRECT ANSWER -
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - CORRECT ANSWER -
Restriction Enzyme Type II
What is necessary to make cDNA? - CORRECT ANSWER - Reverse transcriptase
Number of SNPs in a human genome? - CORRECT ANSWER - 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - CORRECT ANSWER -3
billion base pairs
The polymerization of acrylamide requires: - CORRECT ANSWER - Catalyst (APS or
TEMED) or Light activation
How does agarose gel pore size relate to concentration? - CORRECT ANSWER -
Inversely proportional
What is denatured during a southern blot? - CORRECT ANSWER - Hydrogen bonds
(inter-strand bonds)
