2025Bacteriology Part 2 Questions With Answers Grade A+
1.The steam autoclave method of sterilization:
A. Uses 15 lbs of pressure for 15 minutes
B. Utilizes dry heat for 20 minutes
C. Produces a maximum temperature of 100ºC
D. Requires a source of ethylene oxide: A. Uses 15 lbs of pressure for 15
minutes
2.The biosafety level that includes most common laboratory
microorganisms and involves organisms such as HBV, HIV and enteric
pathogens is:
A. BSL-1
B. BSL-2
C. BSL-3
D. BSL-4: B. BSL-2
3.Seventy (70) % recirculated to the cabinet work area through HEPA;
30% balance can be exhausted through HEPA back into the room or to
outside through a canopy unit:
A. BSC Class I
B. BSC Class II, A1
C. BSC Class II, A2
D. BSC Class II, B1
E. BSC Class II, B2: B. BSC Class II, A1
,4.The proper blood-to-broth ratio for blood cultures to reduce the
antibacterial effect of serum in adults is:
A. 1:2
B. 1:3
C. 1:10
D. 1:30: C. 1:10
5.SPS is used as an anticoagulant for blood cultures because it:
A. Inactivates penicillin and cephalosporins
B. Prevents clumping of red cells
C. Inactivates neutrophils and components of serum complement
D. Facilitates growth of anaerobes: C. Inactivates neutrophils and
components of serum complement
6.Which of the following is the most appropriate method for collecting a
urine specimen from a patient with an indwelling catheter?
, A. Remove the catheter, cut the tip, and submit it for culture
B. Disconnect the catheter from the bag, and collect urine from the
terminal end of the catheter
C. Collect urine directly from the bag
D. Aspirate urine aseptically from the catheter tubing: D. Aspirate urine
asepti- cally from the catheter tubing
7.Which one of the following specimen requests is acceptable?
A. Feces submitted for anaerobic culture
B. Foley catheter tip submitted for aerobic culture
C. Rectal swab submitted for direct smear for gonococci
D. Urine for culture of acid-fast bacilli: D. Urine for culture of acid-fast bacil
8.Transfer stool to if transport will exceed 1 hour.
A. Amies
B. Cary-Blair
C. Eosin-methylene blue agar
D. Stuart's: D. Stuart's
9.For patient preparation for wound swab, wipe the area with:
A. Amies medium
B. Stuart's medium
C. Distilled water
D. Sterile saline or 70% alcohol: D. Sterile saline or 70% alcohol
10.Which of the following groups of specimens would be acceptable
1.The steam autoclave method of sterilization:
A. Uses 15 lbs of pressure for 15 minutes
B. Utilizes dry heat for 20 minutes
C. Produces a maximum temperature of 100ºC
D. Requires a source of ethylene oxide: A. Uses 15 lbs of pressure for 15
minutes
2.The biosafety level that includes most common laboratory
microorganisms and involves organisms such as HBV, HIV and enteric
pathogens is:
A. BSL-1
B. BSL-2
C. BSL-3
D. BSL-4: B. BSL-2
3.Seventy (70) % recirculated to the cabinet work area through HEPA;
30% balance can be exhausted through HEPA back into the room or to
outside through a canopy unit:
A. BSC Class I
B. BSC Class II, A1
C. BSC Class II, A2
D. BSC Class II, B1
E. BSC Class II, B2: B. BSC Class II, A1
,4.The proper blood-to-broth ratio for blood cultures to reduce the
antibacterial effect of serum in adults is:
A. 1:2
B. 1:3
C. 1:10
D. 1:30: C. 1:10
5.SPS is used as an anticoagulant for blood cultures because it:
A. Inactivates penicillin and cephalosporins
B. Prevents clumping of red cells
C. Inactivates neutrophils and components of serum complement
D. Facilitates growth of anaerobes: C. Inactivates neutrophils and
components of serum complement
6.Which of the following is the most appropriate method for collecting a
urine specimen from a patient with an indwelling catheter?
, A. Remove the catheter, cut the tip, and submit it for culture
B. Disconnect the catheter from the bag, and collect urine from the
terminal end of the catheter
C. Collect urine directly from the bag
D. Aspirate urine aseptically from the catheter tubing: D. Aspirate urine
asepti- cally from the catheter tubing
7.Which one of the following specimen requests is acceptable?
A. Feces submitted for anaerobic culture
B. Foley catheter tip submitted for aerobic culture
C. Rectal swab submitted for direct smear for gonococci
D. Urine for culture of acid-fast bacilli: D. Urine for culture of acid-fast bacil
8.Transfer stool to if transport will exceed 1 hour.
A. Amies
B. Cary-Blair
C. Eosin-methylene blue agar
D. Stuart's: D. Stuart's
9.For patient preparation for wound swab, wipe the area with:
A. Amies medium
B. Stuart's medium
C. Distilled water
D. Sterile saline or 70% alcohol: D. Sterile saline or 70% alcohol
10.Which of the following groups of specimens would be acceptable
