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SERIES MICROBIOLOGY LAB PRACTICAL EXAM 1 COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

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Question: hanging drop steps: Ans: 1. Vaseline around cover slip 2. micro organism in center of cover slip 3. place concave slide on cover slip Question: Gram stain steps: Ans: 1. Crystal Violet 2. Gram's Iodine 3. Ethyl Alcohol 4. Safranin Red Question: negative stain steps: SERIES Ans: 1. nigrosin loop ria 4. slide to spread mixture 5. air dry Question: acid-fast stain steps: Ans: 1. carbolfuchsin 2. acid alcohol 3. methylene blue 4. wash & dry Question: simple stain steps Ans: 1. fixed smear slide 2. dyes 3. rinse & dry Question: nutrient broth Ans: -liquid media used to grow bacteria Question: agar Ans: -used to culture microorganisms Question: candle jar SERIES Ans: -used for anaerobic organisms Question: Petri dish Ans: -Used to culture or observe bacteria growth Question: slant Ans: -a medium for which bacteria can grow on. Question: E.coli Gram stain Ans: red colored rods Question: S. epidermidis Gram stain Ans: purple colored grapes Question: B. subtilis Gram stain Ans: purple colored rod

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2025-2026 SERIES
MICROBIOLOGY LAB PRACTICAL EXAM 1 COMPLETE
QUESTIONS AND CORRECT ANSWERS | GRADED
AND THE LATEST SERIES
Wet mount steps:


Ans: 1.water
2. specimen
3. Cover slip over specimen


Question: hanging drop steps:


Ans: 1. Vaseline around cover slip
2. micro organism in center of cover slip
3. place concave slide on cover slip


Question: Gram stain steps:


Ans: 1. Crystal Violet
2. Gram's Iodine
3. Ethyl Alcohol
4. Safranin Red


Question: negative stain steps:

, 2025-2026 SERIES
Ans: 1. nigrosin
2.heat loop
3.bacteria
4. slide to spread mixture
5. air dry


Question: acid-fast stain steps:


Ans: 1. carbolfuchsin
2. acid alcohol
3. methylene blue
4. wash & dry


Question: simple stain steps


Ans: 1. fixed smear slide
2. dyes
3. rinse & dry


Question: nutrient broth


Ans: -liquid media used to grow bacteria


Question: agar


Ans: -used to culture microorganisms


Question: candle jar

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