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SERIES MICROBIOLOGY LAB PRACTICAL #1 REVIEW COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

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Question: Heat Fixing Ans: kills organisms, keeps organisms stuck to slide, allows the specimen to be stained more easily Question: Aseptic Technique Ans: preparing and handling sterile products in a manner that prevents microbial contamination Question: Gram Staining Procedure SERIES Ans: 1. Heat fix bacterial smear 2. Cover with Crystal Violet (Primary Stain) and wait 60 seconds 3. Rinse slide with distilled water until stain is removed 4. Cover with Gram's Iodine (Mordant) and wait for 60 seconds 5. Rinse with distilled water 6. 2-3 drops of Decolorizer (95% Ethanol), or until color is clear, rinse with distilled water 7. Cover with Safranin (Counter Stain) and wait 45 seconds 8. Rinse with distilled water 9. Blot dry with Bibulous paper, observe

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2025-2026 SERIES
MICROBIOLOGY LAB PRACTICAL #1 REVIEW
COMPLETE QUESTIONS AND CORRECT ANSWERS |
GRADED AND THE LATEST SERIES
Bacterial Smear


Ans: Allows better visualization of specimens using microscopy.


Question: Heat Fixing


Ans: kills organisms, keeps organisms stuck to slide, allows the specimen to be
stained more easily


Question: Aseptic Technique


Ans: preparing and handling sterile products in a manner that prevents microbial
contamination


Question: Gram Staining Procedure

, 2025-2026 SERIES
Ans: 1. Heat fix bacterial smear
2. Cover with Crystal Violet (Primary Stain) and wait 60 seconds
3. Rinse slide with distilled water until stain is removed
4. Cover with Gram's Iodine (Mordant) and wait for 60 seconds
5. Rinse with distilled water
6. 2-3 drops of Decolorizer (95% Ethanol), or until color is clear, rinse with distilled
water
7. Cover with Safranin (Counter Stain) and wait 45 seconds
8. Rinse with distilled water
9. Blot dry with Bibulous paper, observe under oil immersion and record results


Question: Capsule Stain Procedure


Ans: 1. Agitate broth culture
2. 2-3 loop fulls of broth culture added to slide and allow to air dry (NOT HEAT
FIXING)
3. Cover with Crystal Violet (Primary Stain) and wait for 2 minutes
4. Rinse with 20% Copper Sulfate Solution (Decolorizer and counter stain)
5. DO NOT RINSE WITH WATER
6. Air dry for a few minutes and DO NOT BLOT
Observe under oil immersion and record results


Question: Why do you not heat fix during the capsule stain procedure?


Ans: Heat will cause the capsule to dislodge


Question: Why do you not rinse with water during the capsule stain procedure?

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