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SERIES MICROBIOLOGY LAB - STAINS COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

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Ans: -Add color to cells for viewing -Highlight entire organism's shape and basic structures -Includes: Methylene blue Safranin Crystal violet Carbolfuchsin Question: Negative stain Ans: -Enhances viewing of morphology, capsules -Bacteria are live -No heat fixing! (no denaturing) -Nigrosin ink -View under oil -Anionic (acidic) simple stain Question: Gram stain Ans: -Determines cell wall type (gram positive or negative) -Differential stain SERIES Question: Gram stain procedure Ans: 1. Crystal violet 2. Iodine (mordant) 3. Alcohol (decolorizer) 4. Safranin (counterstain) 5. Observe under oil Question: Mordant - gram stain Ans: -Increases intensity of crystal violet -Iodine enters cell forms crystal violet-iodine complex (CV-I) -Complex is too large to leave the cell Question: Alcohol - gram negative Ans: -Decolorizer -Dissolves lipids in the outer membrane -Allows CV-I complex to leave cell, making it colorless Question: Safranin - gram stain Ans: -Counter stain -Will only stain gram negative cells Question: Alcohol - gram positive SERIES Ans: -Decolorizer -Dehydrates thick laye

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Institution

Microbiology

Course

Microbiology

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Institution: Microbiology
Course: Microbiology

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Uploaded on: January 9, 2025
Number of pages: 5
Written in: 2024/2025
Type: Exam (elaborations)
Contains: Questions & answers

Subjects

series microbiology lab stains complet

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2025-2026 SERIES
MICROBIOLOGY LAB - STAINS COMPLETE
QUESTIONS AND CORRECT ANSWERS | GRADED
AND THE LATEST SERIES
Simple stain

Ans: -Add color to cells for viewing
-Highlight entire organism's shape and basic structures
-Includes:
Methylene blue
Safranin
Crystal violet
Carbolfuchsin

Question: Negative stain

Ans: -Enhances viewing of morphology, capsules
-Bacteria are live
-No heat fixing! (no denaturing)
-Nigrosin ink
-View under oil
-Anionic (acidic) simple stain

Question: Gram stain

Ans: -Determines cell wall type (gram positive or negative)
-Differential stain

, 2025-2026 SERIES
Question: Gram stain procedure

Ans: 1. Crystal violet
2. Iodine (mordant)
3. Alcohol (decolorizer)
4. Safranin (counterstain)
5. Observe under oil

Question: Mordant - gram stain

Ans: -Increases intensity of crystal violet
-Iodine enters cell forms crystal violet-iodine complex (CV-I)
-Complex is too large to leave the cell

Question: Alcohol - gram negative

Ans: -Decolorizer
-Dissolves lipids in the outer membrane
-Allows CV-I complex to leave cell, making it colorless

Question: Safranin - gram stain

Ans: -Counter stain
-Will only stain gram negative cells

Question: Alcohol - gram positive

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SERIES MICROBIOLOGY LAB - STAINS COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

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