Cytogenetics ASCP TEST|27 QUESTIONS AND ANSWERS

Cytogenetics ASCP TEST|27 QUESTIONS
AND ANSWERS
Day 1 FISH - -1. Denatured in 2xSSC for 2 mins
2. EtOH bath for 2 mins each (70%,80%,90%)
3. Dry very well

- ALL FISH B-CELL - --CEP 4,10,17
-t(9;22) BCR/ABL DUAL COLOR/DUAL FUSION
-t(12;21) ETV6/RUNX1 DUAL COLOR SINGLE FUSION
-11q23 MLL (rearrangement/deletion break apart)

- ALL FISH T-CELL - --t(9;22) BCR/ABL

- AML FISH - --t(8;21)
-INV(16) OR t(16;16)
-11q23 MLL rearrangement/deletion

- 45, X - -Turner syndrome, webbing of the neck, short stature, amnorrhea

- 47, XXY - -Klinefelter syndrome, tall stature, sterility, gynecomastia

- 47, XX, +13 - -Patau syndrome, severe mental retardation, and physical
deformities

- 47, XX,+18 - -Edwards syndrome

- del(4)(p16) - -Wolf Hirschhorn

- del(5)(p15) - -Cri-du-Chat

- del(13)(q14) - -retinoblastoma

- dup(22)(pterq-11) - -cat's eye syndrome

- TEL/AML - --t(12;21)
-ETV6-12
-RUNX1-21

- M2 - --t(8;21)(q22;q22)
ETO/RUNx1
-RUNx1T1-8q21.3
-RUNx1-21q22
-good prognosis