Mock CG(ASCP) Certification exam 1 -
Part 1|100 Questions and Answers
What effects does sodium heparin have on cells?
a. Swells cells
b. Removes water from cells
c. Stimulates mitotic activity
d. Precipitates nucleic acid
e. prevents clotting - -Prevents clotting
- Which of the following is LEAST likely to be toxic to cells?
a. Sodium heparin
b. Lithium heparin
c. Sodium hydroxide
d. Gentamycin - -Sodium heparin
- The optimal temperature for transport of amniotic fluid specimens is?
a. 37 'C
b. 40 'C
c. 0 'C
d. 20 'C - -20 'C
- A pathologist is sending solid tumor specimens to a cytogenetic laboratory
for analysis. What instructions should be given to the pathologist concerning
the preparation and transport of the specimen to insure the highest chance
of successful culture?
a. Ensure the sterility of the specimen by placing it in formalin until it
reaches the laboratory.
b. Ensure that the specimen does not contain necrotic or fatty tissue.
c. Ensure that the specimen is transported on dry ice to prevent
decomposition.
d. All of the above. - -Ensure the specimen does not contain necrotic or fatty
acid
- Why is the villi obtained in CVS procedures cleaned by the technologists?
a. To remove the blood from the sample.
b. To remove dead tissue from the sample.
c. To remove maternal tissue from the sample
d. To expose the rapidly growing cells in the villi. - -To remove maternal
tissue from the sample
- A solid tumor specimen can NOT be shipped from the hospital to a
cytogenetics laboratory in another city due to a major holiday which will not
allow the sample to be shipped via Federal Express Overnight delivery. How
,should the sample be stored so that the cells will remain alive until they can
be delivered and placed in culture?
a. The specimen should be preserved by placing it in formalin until it reaches
the laboratory.
b. The specimen should be placed in media and then in a a 37 'C incubator
until it can be shipped.
c. The specimen should be stored in at -20 'C or on dry ice to prevent cellular
decay.
d. The specimen should be placed in media and then refrigerated until it can
be shipped. - -The specimen should be placed in media and then
refrigerated until it can be shipped.
- Lab C noticed that patient samples from one of their doctors had poor or
no growth as compared to their other doctor's samples. No evidence of
contamination was observed and all material needed for sample collection
and transportation is provided to the doctors by the laboratory. What could
cause this discrepancy in culture viability?
a. Collection technique
b. Incubator systems
c. Toxic plastic containers
d. Culture media - -Collection technique
- Which statement about maternal cell contamination is FALSE?
a. The incidence of MCC is greater when cultures are established from bloody
amniotic fluid samples.
b. The risk of MCC can be reduced by using a larger bone core needle for the
amniocentesis procedure.
c. The use of maternal Q-polymorphisms may be helpful in identifying cases
of MCC.
d. The risk of MCC can be reduced by discarding the first 2-3 cc of aspirated
amniotic fluid. - -The risk of MCC can be reduced by using a larger bone core
needle for the amniocentesis procedure.
- Your laboratory receives specimens form the following cases. Which case
would you assign the highest priority?
a. A 24 year old woman who has had three miscarriages and one normal
child.
b. The 28 year old husband of the woman above.
c. Amnoitc fluid from a 36 year old women's current pregnancy of 18 weeks
gestation.
d. A baby who is in the NICU with a myelomeningocele, a heart defect,
intrauterine growth deficiency, a tightly clenched fist and an unusually
positioned great toe.
e. A 10 year old boy who is small for his age and has small hands, moderate
mental retardation, protruding ears, "droppy" eyelids and a small mouth. - -
A baby who is in the NICU with a myelomeningocele, a heart defect,
, intrauterine growth deficiency, a tightly clenched fist and an unusually
positioned great toe.
- Not all cells received by a Cytogenetics laboratory will grow well in culture
for any number of reasons. When amniotic fluid specimens are collected for
culture, which of the following are safeguards against culture failure?
s. Samples are collected in one syringe
b. The first 2 cc of amniotic fluid are discarded
c. Two flasks are set up with two different media types
d. T12 1/2 flasks are used - -Two flasks are set up with two different media
types
- Which of the following is NOT an advantage that in situ coverslip amniotic
cultures have over flask amniotic fluid cultures?
a. Decreased the turnaround time.
b. Increases the likelihood of distinguishing between true mosaicism and
psuedomosaicisme.
c. Can be used to rule out maternal contamination when the fetus is female.
d. Can determine if an abnormal clone is arising from one or more colonies. -
-Can be used to rule out maternal contamination when the fetus is female.
- Collagenase is used in culture of solid tissues to?
a. Dissociate cells
b. To supplement culture media
c. To prevent mycoplasma contamination
c. To subculture the monolayer cultures - -Dissociate cells
- What microscope is useful in the separation of fetal tissue from maternal
tissue?
a. Brightfield microscope
b. Fluorescent microscope
c. Compound microscope
d. Stereo (dissecting) - microscope - -Stereo (dissecting) - microscope
- Which of the following should be added to modified media before use in
amniotic fluid cultures?
a. Fetal Bovine Serum (FBS)
b. Vitamins
c. Sucrose
d. Gentamycin - -Fetal Bovine Serum (FBS)
- Which the following is often added to media, salt solutions, and enzymes
as a pH indicator?
a. Sodium bicarbonate
b. CO2
c. Hepes buffer
Part 1|100 Questions and Answers
What effects does sodium heparin have on cells?
a. Swells cells
b. Removes water from cells
c. Stimulates mitotic activity
d. Precipitates nucleic acid
e. prevents clotting - -Prevents clotting
- Which of the following is LEAST likely to be toxic to cells?
a. Sodium heparin
b. Lithium heparin
c. Sodium hydroxide
d. Gentamycin - -Sodium heparin
- The optimal temperature for transport of amniotic fluid specimens is?
a. 37 'C
b. 40 'C
c. 0 'C
d. 20 'C - -20 'C
- A pathologist is sending solid tumor specimens to a cytogenetic laboratory
for analysis. What instructions should be given to the pathologist concerning
the preparation and transport of the specimen to insure the highest chance
of successful culture?
a. Ensure the sterility of the specimen by placing it in formalin until it
reaches the laboratory.
b. Ensure that the specimen does not contain necrotic or fatty tissue.
c. Ensure that the specimen is transported on dry ice to prevent
decomposition.
d. All of the above. - -Ensure the specimen does not contain necrotic or fatty
acid
- Why is the villi obtained in CVS procedures cleaned by the technologists?
a. To remove the blood from the sample.
b. To remove dead tissue from the sample.
c. To remove maternal tissue from the sample
d. To expose the rapidly growing cells in the villi. - -To remove maternal
tissue from the sample
- A solid tumor specimen can NOT be shipped from the hospital to a
cytogenetics laboratory in another city due to a major holiday which will not
allow the sample to be shipped via Federal Express Overnight delivery. How
,should the sample be stored so that the cells will remain alive until they can
be delivered and placed in culture?
a. The specimen should be preserved by placing it in formalin until it reaches
the laboratory.
b. The specimen should be placed in media and then in a a 37 'C incubator
until it can be shipped.
c. The specimen should be stored in at -20 'C or on dry ice to prevent cellular
decay.
d. The specimen should be placed in media and then refrigerated until it can
be shipped. - -The specimen should be placed in media and then
refrigerated until it can be shipped.
- Lab C noticed that patient samples from one of their doctors had poor or
no growth as compared to their other doctor's samples. No evidence of
contamination was observed and all material needed for sample collection
and transportation is provided to the doctors by the laboratory. What could
cause this discrepancy in culture viability?
a. Collection technique
b. Incubator systems
c. Toxic plastic containers
d. Culture media - -Collection technique
- Which statement about maternal cell contamination is FALSE?
a. The incidence of MCC is greater when cultures are established from bloody
amniotic fluid samples.
b. The risk of MCC can be reduced by using a larger bone core needle for the
amniocentesis procedure.
c. The use of maternal Q-polymorphisms may be helpful in identifying cases
of MCC.
d. The risk of MCC can be reduced by discarding the first 2-3 cc of aspirated
amniotic fluid. - -The risk of MCC can be reduced by using a larger bone core
needle for the amniocentesis procedure.
- Your laboratory receives specimens form the following cases. Which case
would you assign the highest priority?
a. A 24 year old woman who has had three miscarriages and one normal
child.
b. The 28 year old husband of the woman above.
c. Amnoitc fluid from a 36 year old women's current pregnancy of 18 weeks
gestation.
d. A baby who is in the NICU with a myelomeningocele, a heart defect,
intrauterine growth deficiency, a tightly clenched fist and an unusually
positioned great toe.
e. A 10 year old boy who is small for his age and has small hands, moderate
mental retardation, protruding ears, "droppy" eyelids and a small mouth. - -
A baby who is in the NICU with a myelomeningocele, a heart defect,
, intrauterine growth deficiency, a tightly clenched fist and an unusually
positioned great toe.
- Not all cells received by a Cytogenetics laboratory will grow well in culture
for any number of reasons. When amniotic fluid specimens are collected for
culture, which of the following are safeguards against culture failure?
s. Samples are collected in one syringe
b. The first 2 cc of amniotic fluid are discarded
c. Two flasks are set up with two different media types
d. T12 1/2 flasks are used - -Two flasks are set up with two different media
types
- Which of the following is NOT an advantage that in situ coverslip amniotic
cultures have over flask amniotic fluid cultures?
a. Decreased the turnaround time.
b. Increases the likelihood of distinguishing between true mosaicism and
psuedomosaicisme.
c. Can be used to rule out maternal contamination when the fetus is female.
d. Can determine if an abnormal clone is arising from one or more colonies. -
-Can be used to rule out maternal contamination when the fetus is female.
- Collagenase is used in culture of solid tissues to?
a. Dissociate cells
b. To supplement culture media
c. To prevent mycoplasma contamination
c. To subculture the monolayer cultures - -Dissociate cells
- What microscope is useful in the separation of fetal tissue from maternal
tissue?
a. Brightfield microscope
b. Fluorescent microscope
c. Compound microscope
d. Stereo (dissecting) - microscope - -Stereo (dissecting) - microscope
- Which of the following should be added to modified media before use in
amniotic fluid cultures?
a. Fetal Bovine Serum (FBS)
b. Vitamins
c. Sucrose
d. Gentamycin - -Fetal Bovine Serum (FBS)
- Which the following is often added to media, salt solutions, and enzymes
as a pH indicator?
a. Sodium bicarbonate
b. CO2
c. Hepes buffer
