MCB 252 Exam 3 Lon Capa Exam
Questions and Answers
Which of the following is/are a global response(s) to signaling (or lack of signaling in
some case(s) discussed in lecture? Select the best single answer.Incorrect cell
differentiation - correct answer-cell survive, cell grow and division, cell differentiation,
and apoptosis
Which of the following is correct with regard to the two statements below?
i = Adult stem cells are pluripotent.
ii = When a stem cell divides one of the two resulting cells is a stem cell. - correct
answer-i is FALSE, and ii is TRUE.
In Xenopus laevis, proper tissue development requires which of the following? - correct
answer-All of the above (regulated cell division, signaling between cells, and apoptosis)
As discussed in lecture, apoptosis can occur in response to what? Select the best single
answer. - correct answer-Signaling in normal development and cellular damage,
especially DNA damage
As discussed in lecture, approximately how many cell types are there in human cells, at
least for our comparative purposes this semester? Choose the best single answer. -
correct answer-210
Approximately how many cells are there in the human body? - correct answer-10^14
What determines the shape of a eukaryotic cell? - correct answer-The shape of the
cytoskeleton
From which germ layer does the neural tube form during Xenopus development? -
correct answer-Ectoderm
Which of the two statements below is/are TRUE, and which is/are FALSE?
i = Exon shuffling occurs at the level of DNA in the genome; whereas, splicing occurs at
the level of RNA.
ii = In lecture, we discussed the modular construction of some genes and the evolution
of genes (and proteins) by the addition of domains. As described in lecture, this process
occurs in prokaryotes and eukaryotes - correct answer-i is TRUE, and ii is FALSE.
, Which property of eukaryotic gene regulatory regions, as discussed in lecture, allowed
for the evolution complex regulatory regions? - correct answer-That enhancers can act
over long distances
Which of the following is/are often properties of protein domains? - correct answer-
When properly folded, the N- and C- termini often point in opposite directions.
A version of the figure below was discussed in lecture.
Which of the two statements below is/are TRUE, and which is/are FALSE?
i = α-globin gene A3 is more closely related to myoglobin gene M1 than it is to β-globin
gene B1.ii = β-globin gene B5 is more closely related to β-globin gene B1 than it is to α-
globin gene A1. - correct answer-i is FALSE, and ii is TRUE.
Which of the following statements is/are TRUE, and which is/are FALSE?
i = As described in lecture, a polyclonal antibody contains many different antibodies,
each of which recognizes a distinct epitope on the surface of an antigen.
ii = As described in lecture, when purifying a protein of interest one needs an assay that
is specific for that particular protein. - correct answer-Both i and ii are TRUE.
In order to determine the structure of a protein by X-ray crystallography you need which
of the following? - correct answer-The protein of interest needs to be purified, and you
need to know the amino acid sequence of the protein of interest.
You are carrying out in vitro chemical reactions using an enzyme that produces a
deadly cyanide gas when you add the substrate for that enzyme. You have produced a
monoclonal antibody and a polyclonal antibody against your cyanide-producing enzyme.
Normally when you work with your enzyme you wear protective gear to prevent you
from inhaling the deadly gas that is produced. However, today you accidentally add the
substrate to the enzyme before you put on your protective gear and rapidly find your -
correct answer-The tube containing the polyclonal antibody.
In 2008 the Nobel Prize in Chemistry went to the researchers involved in the
development of GFP as an experimental tool for visualization of proteins and expression
patterns. What is the advantage discussed in lecture of using GFP rather than
immunofluorescence? - correct answer-GFP allows visualization of living cells and
organisms.
Match the term below with the correct description.
i = transgenics
ii = transformation
iii = transfection
X = When DNA is introduced into cells, and that DNA is only transiently maintained in
those cells.
Y = When DNA is integrated into a chromosome and maintained in all the cells of a
living organism.
Questions and Answers
Which of the following is/are a global response(s) to signaling (or lack of signaling in
some case(s) discussed in lecture? Select the best single answer.Incorrect cell
differentiation - correct answer-cell survive, cell grow and division, cell differentiation,
and apoptosis
Which of the following is correct with regard to the two statements below?
i = Adult stem cells are pluripotent.
ii = When a stem cell divides one of the two resulting cells is a stem cell. - correct
answer-i is FALSE, and ii is TRUE.
In Xenopus laevis, proper tissue development requires which of the following? - correct
answer-All of the above (regulated cell division, signaling between cells, and apoptosis)
As discussed in lecture, apoptosis can occur in response to what? Select the best single
answer. - correct answer-Signaling in normal development and cellular damage,
especially DNA damage
As discussed in lecture, approximately how many cell types are there in human cells, at
least for our comparative purposes this semester? Choose the best single answer. -
correct answer-210
Approximately how many cells are there in the human body? - correct answer-10^14
What determines the shape of a eukaryotic cell? - correct answer-The shape of the
cytoskeleton
From which germ layer does the neural tube form during Xenopus development? -
correct answer-Ectoderm
Which of the two statements below is/are TRUE, and which is/are FALSE?
i = Exon shuffling occurs at the level of DNA in the genome; whereas, splicing occurs at
the level of RNA.
ii = In lecture, we discussed the modular construction of some genes and the evolution
of genes (and proteins) by the addition of domains. As described in lecture, this process
occurs in prokaryotes and eukaryotes - correct answer-i is TRUE, and ii is FALSE.
, Which property of eukaryotic gene regulatory regions, as discussed in lecture, allowed
for the evolution complex regulatory regions? - correct answer-That enhancers can act
over long distances
Which of the following is/are often properties of protein domains? - correct answer-
When properly folded, the N- and C- termini often point in opposite directions.
A version of the figure below was discussed in lecture.
Which of the two statements below is/are TRUE, and which is/are FALSE?
i = α-globin gene A3 is more closely related to myoglobin gene M1 than it is to β-globin
gene B1.ii = β-globin gene B5 is more closely related to β-globin gene B1 than it is to α-
globin gene A1. - correct answer-i is FALSE, and ii is TRUE.
Which of the following statements is/are TRUE, and which is/are FALSE?
i = As described in lecture, a polyclonal antibody contains many different antibodies,
each of which recognizes a distinct epitope on the surface of an antigen.
ii = As described in lecture, when purifying a protein of interest one needs an assay that
is specific for that particular protein. - correct answer-Both i and ii are TRUE.
In order to determine the structure of a protein by X-ray crystallography you need which
of the following? - correct answer-The protein of interest needs to be purified, and you
need to know the amino acid sequence of the protein of interest.
You are carrying out in vitro chemical reactions using an enzyme that produces a
deadly cyanide gas when you add the substrate for that enzyme. You have produced a
monoclonal antibody and a polyclonal antibody against your cyanide-producing enzyme.
Normally when you work with your enzyme you wear protective gear to prevent you
from inhaling the deadly gas that is produced. However, today you accidentally add the
substrate to the enzyme before you put on your protective gear and rapidly find your -
correct answer-The tube containing the polyclonal antibody.
In 2008 the Nobel Prize in Chemistry went to the researchers involved in the
development of GFP as an experimental tool for visualization of proteins and expression
patterns. What is the advantage discussed in lecture of using GFP rather than
immunofluorescence? - correct answer-GFP allows visualization of living cells and
organisms.
Match the term below with the correct description.
i = transgenics
ii = transformation
iii = transfection
X = When DNA is introduced into cells, and that DNA is only transiently maintained in
those cells.
Y = When DNA is integrated into a chromosome and maintained in all the cells of a
living organism.
