BMS 528- CH. 24: transcription and post transcriptional processing
Study online at https://quizlet.com/_gc7793
1. Information transfer in the cell:
cell division
RNA
REVERSE TRAN-
DNA replication - DNA template-directed duplication
SCRIPTION
of the genome prior to
mRNA
Transcription - DNA template-directed biosynthesis
of
- RNA template-directed biosynthesis of
DNA
Translation - template-directed biosynthesis of
proteins
2. Jacob and Monad Predicted Characteristics of mRNA: lactose
digested
decreases
In the presence of both glucose and glucose,
Ecoli preferentially metabolized glucose. Upon ex-
haustion of glucose supply three enzymes of
lactose metabolism are synthesized and lactose is
.
Upon addition of glucose induction (activation) is
re- versed and galactose related enzyme activity .
Data lead to mRNA predictions
3. Jacob and Monad Predicted Characteristics of mRNA: high
turned on
larger
1) Rate of synthesis and degradation of messenger identical
is . mRNA is made as genes are (when
needed) and turned off when no longer required.
2) Because they expected the messenger (mRNA)
to be a copy of two or more genes they expected
the molecule to be .
3) If mRNA was a complimentary copy of DNA its
1/
22
, BMS 528- CH. 24: transcription and post transcriptional processing
Study online at https://quizlet.com/_gc7793
nucleotide sequence should be to a portion of
one of the DNA strands.
4. Bacterial genetics predicted messenger RNA to be metabolically
a collection of RNAs, present in low abun- ac- tive
dance, heterogeneous in size, and to sequence complementary
regions in the DNA genome
5. mRNA is a set of genes needed to collectively certain
perform a function
regulator
The encodes for the repressor molecule, gene
which can either activate or deactivate the conformation
operator. al operator
protein
If inducer binds to repressor, it undergoes
change and no longer binds to
Transcription can then begin DNA -> mRNA->
6. Evidence of mRNA was first observed in and_____T2, T4
bacteriophages. expression
synthesized,
Infection of virus stops all of host genes/ RNA. de- graded
DNA , AT
Radio labeled RNA was found to be synthesized.
Had two properties
1) It was unstable. Quickly and
2) RNA was a product of . The radio labeled RNA
was rich in when in comparison DNA was rich in
GC.
7. Detection of mRNA using pulse labeling and RNA
sedimen- tation: size and shape
A centrifuge tube containing a sucrose gradient
(5-20%) before (left) and after centrifugation (right)
2/
22
, BMS 528- CH. 24: transcription and post transcriptional processing
Study online at https://quizlet.com/_gc7793
shows how is separated by this technique
The S values refer to the sedimentation
coefficients of the various particles in Svedberg
units (S units depend on and )
8. Detection of mRNA using pulse labeling and siz
sedimen- tation: e
UV
Centrifuge separates molecules on . S value gene
is related to size.
16s and 23s represent rRNA, 4s represents tRNA.
A hole at the bottom of tube allows bands to drain
and are analyzed by spec.
The new mRNA had varying sizes (heterogeneously
distributed). Size of mRNA depends on the size of
the
.
9. Detection of mRNA using pulse labeling and various sizes
sedimen- tation:
half life
(Left panel) Several prominent peaks (black trace) sizes
correspond to rRNA and tRNA; the radioactivity (red gene
trace) is distributed heterogeneously throughout, translati
in- dicating a collection of mRNAs of on
(Right panel) A short pulse (orange) highlights the
ex- istence of a variety of RNA species; continued
growth in nonradiolabeled medium shows only the
peaks cor- responding the rRNA and tRNA; the
signal of other RNAs has disappeared, therefore
mRNAs have a short
(t1/2 = 2-3 minutes)
Conclusions:
- mRNA is found in various ; size is related to
the size of the
3/
22
Study online at https://quizlet.com/_gc7793
1. Information transfer in the cell:
cell division
RNA
REVERSE TRAN-
DNA replication - DNA template-directed duplication
SCRIPTION
of the genome prior to
mRNA
Transcription - DNA template-directed biosynthesis
of
- RNA template-directed biosynthesis of
DNA
Translation - template-directed biosynthesis of
proteins
2. Jacob and Monad Predicted Characteristics of mRNA: lactose
digested
decreases
In the presence of both glucose and glucose,
Ecoli preferentially metabolized glucose. Upon ex-
haustion of glucose supply three enzymes of
lactose metabolism are synthesized and lactose is
.
Upon addition of glucose induction (activation) is
re- versed and galactose related enzyme activity .
Data lead to mRNA predictions
3. Jacob and Monad Predicted Characteristics of mRNA: high
turned on
larger
1) Rate of synthesis and degradation of messenger identical
is . mRNA is made as genes are (when
needed) and turned off when no longer required.
2) Because they expected the messenger (mRNA)
to be a copy of two or more genes they expected
the molecule to be .
3) If mRNA was a complimentary copy of DNA its
1/
22
, BMS 528- CH. 24: transcription and post transcriptional processing
Study online at https://quizlet.com/_gc7793
nucleotide sequence should be to a portion of
one of the DNA strands.
4. Bacterial genetics predicted messenger RNA to be metabolically
a collection of RNAs, present in low abun- ac- tive
dance, heterogeneous in size, and to sequence complementary
regions in the DNA genome
5. mRNA is a set of genes needed to collectively certain
perform a function
regulator
The encodes for the repressor molecule, gene
which can either activate or deactivate the conformation
operator. al operator
protein
If inducer binds to repressor, it undergoes
change and no longer binds to
Transcription can then begin DNA -> mRNA->
6. Evidence of mRNA was first observed in and_____T2, T4
bacteriophages. expression
synthesized,
Infection of virus stops all of host genes/ RNA. de- graded
DNA , AT
Radio labeled RNA was found to be synthesized.
Had two properties
1) It was unstable. Quickly and
2) RNA was a product of . The radio labeled RNA
was rich in when in comparison DNA was rich in
GC.
7. Detection of mRNA using pulse labeling and RNA
sedimen- tation: size and shape
A centrifuge tube containing a sucrose gradient
(5-20%) before (left) and after centrifugation (right)
2/
22
, BMS 528- CH. 24: transcription and post transcriptional processing
Study online at https://quizlet.com/_gc7793
shows how is separated by this technique
The S values refer to the sedimentation
coefficients of the various particles in Svedberg
units (S units depend on and )
8. Detection of mRNA using pulse labeling and siz
sedimen- tation: e
UV
Centrifuge separates molecules on . S value gene
is related to size.
16s and 23s represent rRNA, 4s represents tRNA.
A hole at the bottom of tube allows bands to drain
and are analyzed by spec.
The new mRNA had varying sizes (heterogeneously
distributed). Size of mRNA depends on the size of
the
.
9. Detection of mRNA using pulse labeling and various sizes
sedimen- tation:
half life
(Left panel) Several prominent peaks (black trace) sizes
correspond to rRNA and tRNA; the radioactivity (red gene
trace) is distributed heterogeneously throughout, translati
in- dicating a collection of mRNAs of on
(Right panel) A short pulse (orange) highlights the
ex- istence of a variety of RNA species; continued
growth in nonradiolabeled medium shows only the
peaks cor- responding the rRNA and tRNA; the
signal of other RNAs has disappeared, therefore
mRNAs have a short
(t1/2 = 2-3 minutes)
Conclusions:
- mRNA is found in various ; size is related to
the size of the
3/
22
