BMS 525 Exam 1 Question and answers
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The study of Gene expression - correct answer ✔Molecular Biology Def
Nuclease - correct answer ✔enzyme that degrades nucleic acids via
hydrolyzing and ester bond
Tm - correct answer ✔Temperature at which 50% of the DNA strand is
denatured
Agarose Gel Electrophoresis - correct answer ✔separates DNA molecules
by size
-EtBr for staining
Blotting steps - correct answer ✔1. isolate DNA (restriction enzymes)
2. Run electrophoresis
3. Transfer to blot
4. Hybridize with DNA probe
PCR steps - correct answer ✔1. Denaturation 30s
2. Annealing 30s
3. Extension 30s then 5 mins
,Run product on agarose gel and stain with EtBr
PCR requires (5) - correct answer ✔-Template DNA
-Primers
-dNTPs
-Taq polymerase
-bugger (Mg2+)
RT-PCR - correct answer ✔Uses Reverse transcriptase to create cDNA for
PCR.
-measures RNA expression levels
qPCR (Real-time) - correct answer ✔Detects PCR products in real time
-Probes that fluoresce is used for detection
-detection during exponential phase of PCR
-Optical module detects fluorescence and computer quantifies the data
qPCR probes/dyes - correct answer ✔TaqMan - 5' reporter is cleaved off for
fluorescence
SYBR green Dye - binds to dsDNA and fluoresces
, Structure of DNA polymer - correct answer ✔
Mutations in DNA - correct answer ✔Substitution - replacing a base
Insertion - adding a base
Deletion - removing a base
Framshift - correct answer ✔insertion or deletion in the CODING REGION
that alters the reading frame
Null mutation - correct answer ✔results in complete elimination of the gene
(deletion)
Meselson-Stahl Experiment
Research question?
Expected Results?
Conclusion? - correct answer ✔Goal: which type of DNA replication occurs?
Results Conservative:
-after one generation, one heavy band and one light band
-after two generations, one heavy and one light
Results Semiconservative:
-after one generation, one intermediate hybrid band
-after two generations, one light band and one hybrid band
rated A+
The study of Gene expression - correct answer ✔Molecular Biology Def
Nuclease - correct answer ✔enzyme that degrades nucleic acids via
hydrolyzing and ester bond
Tm - correct answer ✔Temperature at which 50% of the DNA strand is
denatured
Agarose Gel Electrophoresis - correct answer ✔separates DNA molecules
by size
-EtBr for staining
Blotting steps - correct answer ✔1. isolate DNA (restriction enzymes)
2. Run electrophoresis
3. Transfer to blot
4. Hybridize with DNA probe
PCR steps - correct answer ✔1. Denaturation 30s
2. Annealing 30s
3. Extension 30s then 5 mins
,Run product on agarose gel and stain with EtBr
PCR requires (5) - correct answer ✔-Template DNA
-Primers
-dNTPs
-Taq polymerase
-bugger (Mg2+)
RT-PCR - correct answer ✔Uses Reverse transcriptase to create cDNA for
PCR.
-measures RNA expression levels
qPCR (Real-time) - correct answer ✔Detects PCR products in real time
-Probes that fluoresce is used for detection
-detection during exponential phase of PCR
-Optical module detects fluorescence and computer quantifies the data
qPCR probes/dyes - correct answer ✔TaqMan - 5' reporter is cleaved off for
fluorescence
SYBR green Dye - binds to dsDNA and fluoresces
, Structure of DNA polymer - correct answer ✔
Mutations in DNA - correct answer ✔Substitution - replacing a base
Insertion - adding a base
Deletion - removing a base
Framshift - correct answer ✔insertion or deletion in the CODING REGION
that alters the reading frame
Null mutation - correct answer ✔results in complete elimination of the gene
(deletion)
Meselson-Stahl Experiment
Research question?
Expected Results?
Conclusion? - correct answer ✔Goal: which type of DNA replication occurs?
Results Conservative:
-after one generation, one heavy band and one light band
-after two generations, one heavy and one light
Results Semiconservative:
-after one generation, one intermediate hybrid band
-after two generations, one light band and one hybrid band
