(PRACTICE TEST Q'S TO USE FOR TEST PREP)
QUESTIONS WITH COMPLETE SOLUTIONS!!
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, 400
The suggested level for most constitutional specimen is 550, but 400 is the
lowest acceptible level
See CAP regulation CYG.42200
Can rapidly detect sex chromosomes or aneuploidy of chromosomes.
Requires no culture
Requires only simple microscope analysis.
3. Rubinstein-Taybi
Associated with an autosomal dominant mutation in the CREB or CREBBP gene(s)
Rarely assocated with a deletion in 16p13.3 (12% of cases, not the predominant
cause, detected by FISH)
Presents as facial dysmorphism, broad thumbs, broad big toes, and growth and
mental retardation
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To avoid culture failure, monitoring reagents is crucial. When
logging a culture failure, what is the MOST important to document?
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Expiration dates of reagents
The enzymatic activity increases
used
, Increading the drying time by using
The number of colonies present warm slides
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If a tech with a cold and sneezing does not take proper precautions
while setting up amniotic fluid samples, the specimen could become
contaminated with:
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Unbalanced
("der" means "a derivative chromosome"; "t" translocation. This indi. has only one
copy of chromosome 9q from band 9q33 to 9qter), therefore is unbalanced
Mycoplasma
--> the most common contaminant by a sick tech and can be detected
through a specific stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to
avoid contamination
Report the result as this is a true mosaic case and suggest:
repeating amniocentesis,
repeating a detailed ultrasound, or
request percutaneous umbilical blood sample (PUBS)
, Confirm the abnormality in other cells in the colony.
Confirm the abnormality in another culture.
Confirm the abnormality in other colonies on the same coverslip.
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In fluorescent microscopy, the exciter and barrier filters used
depend on ___ ?
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ID # of the patient, Stain - because the
Scope used to capture the cell wavelength for emission can
analyzed, and vary per type of fluorescent
the coordinates of the met stain used (i.e. DAPI)
The camera is not parfocal (in focus 46 -- because the isochromosome
with the microscpe objectives) with replaces the normal X, causing
the microscope Turner syndrome
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