MOLECULAR BIOLOGY ASCP
PRACTICE QUESTIONS PART 1 WITH
100% CORRECT ANSWERS
This refers to a means by which large quantities of DNA can be amplified from a small
amount of DNA template:
A. PCR
B. DNA mass production
C. DNA synthesis
D. DNA birth - ANSWER-PCR
With regards to PCR, which of the following is NOT accurate?
A. Denature strands at 94 degrees
B. Base pairing of primers at 60 degrees
C. Elongation of primers using Taq polymerase at 72 degrees
D. Filtration of DNA at 40 degrees - ANSWER-Filtration of DNA at 40 degrees
Which of the following PCR techniques is the best one for detecting point mutation in
DNA?
A. RT PCR
B. PCR
C. Allele-specific PCR
D. Anchored PCR - ANSWER-Allele-Specific PCR
What happens after many cycles of PCR?
A. Exponential Amplification
B. Diminished Amplification
C. Product lysing
D. DNA translation - ANSWER-Exponential Amplification
Which of the following is NOT true about the Taq DNA polymerase?
A. It allows for the ability to use PCR to automate the process of amplification
B. It can be heat resistant
C. It is ubiquitous in nature except for the hot springs
D. It is most efficient at a certain optimal temperature. - ANSWER-C
The PCR technique used to specifically amplify only one strand of the target DNA by
using only one unequal primer concentration is:
A. qPCR
B. Asymmetric PCR
C. Anchored PCR
D. Nested PCR - ANSWER-Asymmetric PCR
, Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100
degrees for an hour. What is the likely result of her PCR?
A. She will get much more amplified DNA product
B. She will not get a significant amplified DNA product
C. She will only get minimal synthesis
D. She will likely not get any synthesis - ANSWER-She will likely not get any synthesis
True or False: Short strands of DNA usually migrate faster through gel than long ones -
ANSWER-True
Which of the following is NOT true about reverse transcription PCR (RT-PCR):
A. Can be used to detect RNA
B. Used to convert mRNA to cDNA
C. Retroviruses use reverse transcriptase to convert ssRNA to dsDNA
D. Coronaviruses use reverse transcriptase to convert ssDNA to ssRNA - ANSWER-
Coronaviruses use reverse transcriptase to convert ssDNA to ssRNA
The most efficient PCR technique that uses primer mix to detect deletions or
duplications in a large gene is:
A. Anchored PCR
B. Nested PCR
C. Miniprimer PCR
D. Multiplex PCR - ANSWER-Multiplex PCR
Which of the following is NOT true about quantitative real-time PCR (qRT-PCR)?
A. It uses a fluorescent dye to quantify the abundance of transcripts
B. It uses an instrument to detect fluorescence of probed targets
C. Quantitative in nature
D. It shows only the plateaued number of copies of DNA made every 30 minutes -
ANSWER-It shows only the plateaued number of copies of DNA made every 30 minutes
The PCR procedure, where Taq polymerase is added after the rest of the PCR
components are heated to the DNA melting temperature, so as to avoid non-specific
amplification at lower temperatures is:
A. Asymmetric PCR
B. Hotstart PCR
C. Multiplex PCR
D. Nested PCR - ANSWER-Hotstart PCR
Which of the following is NOT true about HCV Genotypes?
A. The genotype determines treatment protocol
B. HCV 1 is most common and most difficult to treat
C. HCV 1 is associated with a less severe liver disease than 2 and 3
PRACTICE QUESTIONS PART 1 WITH
100% CORRECT ANSWERS
This refers to a means by which large quantities of DNA can be amplified from a small
amount of DNA template:
A. PCR
B. DNA mass production
C. DNA synthesis
D. DNA birth - ANSWER-PCR
With regards to PCR, which of the following is NOT accurate?
A. Denature strands at 94 degrees
B. Base pairing of primers at 60 degrees
C. Elongation of primers using Taq polymerase at 72 degrees
D. Filtration of DNA at 40 degrees - ANSWER-Filtration of DNA at 40 degrees
Which of the following PCR techniques is the best one for detecting point mutation in
DNA?
A. RT PCR
B. PCR
C. Allele-specific PCR
D. Anchored PCR - ANSWER-Allele-Specific PCR
What happens after many cycles of PCR?
A. Exponential Amplification
B. Diminished Amplification
C. Product lysing
D. DNA translation - ANSWER-Exponential Amplification
Which of the following is NOT true about the Taq DNA polymerase?
A. It allows for the ability to use PCR to automate the process of amplification
B. It can be heat resistant
C. It is ubiquitous in nature except for the hot springs
D. It is most efficient at a certain optimal temperature. - ANSWER-C
The PCR technique used to specifically amplify only one strand of the target DNA by
using only one unequal primer concentration is:
A. qPCR
B. Asymmetric PCR
C. Anchored PCR
D. Nested PCR - ANSWER-Asymmetric PCR
, Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100
degrees for an hour. What is the likely result of her PCR?
A. She will get much more amplified DNA product
B. She will not get a significant amplified DNA product
C. She will only get minimal synthesis
D. She will likely not get any synthesis - ANSWER-She will likely not get any synthesis
True or False: Short strands of DNA usually migrate faster through gel than long ones -
ANSWER-True
Which of the following is NOT true about reverse transcription PCR (RT-PCR):
A. Can be used to detect RNA
B. Used to convert mRNA to cDNA
C. Retroviruses use reverse transcriptase to convert ssRNA to dsDNA
D. Coronaviruses use reverse transcriptase to convert ssDNA to ssRNA - ANSWER-
Coronaviruses use reverse transcriptase to convert ssDNA to ssRNA
The most efficient PCR technique that uses primer mix to detect deletions or
duplications in a large gene is:
A. Anchored PCR
B. Nested PCR
C. Miniprimer PCR
D. Multiplex PCR - ANSWER-Multiplex PCR
Which of the following is NOT true about quantitative real-time PCR (qRT-PCR)?
A. It uses a fluorescent dye to quantify the abundance of transcripts
B. It uses an instrument to detect fluorescence of probed targets
C. Quantitative in nature
D. It shows only the plateaued number of copies of DNA made every 30 minutes -
ANSWER-It shows only the plateaued number of copies of DNA made every 30 minutes
The PCR procedure, where Taq polymerase is added after the rest of the PCR
components are heated to the DNA melting temperature, so as to avoid non-specific
amplification at lower temperatures is:
A. Asymmetric PCR
B. Hotstart PCR
C. Multiplex PCR
D. Nested PCR - ANSWER-Hotstart PCR
Which of the following is NOT true about HCV Genotypes?
A. The genotype determines treatment protocol
B. HCV 1 is most common and most difficult to treat
C. HCV 1 is associated with a less severe liver disease than 2 and 3
