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tRNA - ✔✔Transfer RNA
Decodes mRNA into a protein
Functions at the ribosome during Translation
rRNA - ✔✔Ribosomal RNA
Essential for protein synthesis
Predominant material in a ribosome (60%)
Catalyzes polypeptide formation (Ribozyme)
mRNA - ✔✔Messanger RNA
Conveys genetic info from DNA to ribosome
Specifies the amino acid sequence of protein products
Which type of RNA catalyzes the synthesis of proteins? - ✔✔rRNA
What enzyme complex makes DNA from RNA? - ✔✔Reverse transcriptase
The first half of DNA restriction site has the sequence AACC, what are the
next four bases on the same chain? - ✔✔GGTT
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,What percentage of human chromosomal DNA codes for proteins? - ✔✔1-
2%
DNA replication involves the nucleophilic attack on a deoxynucleotide
phosphate by a: - ✔✔3' hydroxyl group of deoxyribose
What additional factor does lagging strand DNA synthesis require that is
not needed for leading strand synthesis? - ✔✔a DNA ligase
Various forms of DNA that vary in the degree of winding are called: -
✔✔Topoisomers
If the first four bases are ATGC, what are the next four bases on the SAME
DNA strand? - ✔✔GCAT
During DNA replication the lagging DNA strand is READ at its: - ✔✔3' end
In addition to hydrogen bonding, the stability of double helix between
complementary DNA chains is promoted by? - ✔✔Aromatic stacking
In the structure of ATP the inner phosphate group is joined to the 5' carbon
of ribose by what type of bond? - ✔✔Phosphoester
What feature is found in DNA synthesis but NOT in RNA synthesis? -
✔✔Repair mechanisms which remove incorrect bases
Which statement is TRUE about ribosomes? - ✔✔Protein synthesis is
catalyzed by ribosomal RNA
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, The process of cutting DNA strands at specific sites is accomplished by? -
✔✔Restriction endonucleases
Which of the following is needed for replication of DNA but NOT for
transcription of DNA to RNA? - ✔✔a primer strand
In bacteria a protein with 1200 amino acids might be coded by a sequence
of DNA with an open reading frame of - ✔✔3600 bases (3 for each AA)
Which of the following statements is TRUE about the coding strand of
DNA? - ✔✔It has the same base sequence as the transcript except for the
substitution of U's
The signals at the ends of introns in immature mRNA, that code for their
removal are: - ✔✔GU and AG
If a restriction site in a circular plasmid has the sequence GGAATTCC, and
the endonucleas cuts between A and A, how many unpaired bases will
occur on each strand at the opening that is produced? - ✔✔2
In RNA synthesis to which base in the DNA is the U in the RNA product
paired? - ✔✔A
At neutral pH, DNA carries a negative charge because of the -
✔✔Phosphate
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