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BIOC 3021 Exam Study Guide

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©FYNDLAY EXAM SOLUTIONS 2024/2025 ALL RIGHTS RESERVED. 1 | P a g e BIOC 3021 Exam Study Guide. tRNA - answerTransfer RNA Decodes mRNA into a protein Functions at the ribosome during Translation rRNA - answerRibosomal RNA Essential for protein synthesis Predominant material in a ribosome (60%) Catalyzes polypeptide formation (Ribozyme) mRNA - answerMessanger RNA Conveys genetic info from DNA to ribosome Specifies the amino acid sequence of protein products Which type of RNA catalyzes the synthesis of proteins? - answerrRNA What enzyme complex makes DNA from RNA? - answerReverse transcriptase The first half of DNA restriction site has the sequence AACC, what are the next four bases on the same chain? - answerGGTT What percentage of human chromosomal DNA codes for proteins? - answer1-2% DNA replication involves the nucleophilic attack on a deoxynucleotide phosphate by a: - answer3' hydroxyl group of deoxyribose What additional factor does lagging strand DNA synthesis require that is not needed for leading strand synthesis? - answera DNA ligase Various forms of DNA that vary in the degree of winding are called: - answerTopoisomers ©FYNDLAY EXAM SOLUTIONS 2024/2025 ALL RIGHTS RESERVED. 2 | P a g e If the first four bases are ATGC, what are the next four bases on the SAME DNA strand? - answerGCAT During DNA replication the lagging DNA strand is READ at its: - answer3' end In addition to hydrogen bonding, the stability of double helix between complementary DNA chains is promoted by? - answerAromatic stacking In the structure of ATP the inner phosphate group is joined to the 5' carbon of ribose by what type of bond? - answerPhosphoester What feature is found in DNA synthesis but NOT in RNA synthesis? - answerRepair mechanisms which remove incorrect bases Which statement is TRUE about ribosomes? - answerProtein synthesis is catalyzed by ribosomal RNA The process of cutting DNA strands at specific sites is accomplished by? - answerRestriction endonucleases Which of the following is needed for replication of DNA but NOT for transcription of DNA to RNA? - answera primer strand In bacteria a protein with 1200 amino acids might be coded by a sequence of DNA with an open reading frame of - answer3600 bases (3 for each AA) Which of the following statements is TRUE about the coding strand of DNA? - answerIt has the same base sequence as the transcript except for the substitution of U's The signals at the ends of introns in immature mRNA, that code for their removal are: - answerGU and AG If a restriction site in a circular plasmid has the sequence GGAATTCC, and the endonucleas cuts between A and A, how many unpaired bases will occur on each strand at the opening that is produced? - answer2 In RNA synthesis to which base in the DNA is the U in the RNA product paired? - answerA At neutral pH, DNA carries a negative charge because of the - answerPhosphate The critical ingredient in PCR which allows for multiple rounds of DNA synthesis is? - answerThe heat resistant DNA polymerase The hydrolytic enzymes, used in cloning experiments, which make cuts at distinct sites in DNA are called? - answerrestriction endonucleases

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©FYNDLAY EXAM SOLUTIONS 2024/2025

ALL RIGHTS RESERVED.




BIOC 3021 Exam Study Guide.


tRNA - answer✔Transfer RNA
Decodes mRNA into a protein
Functions at the ribosome during Translation

rRNA - answer✔Ribosomal RNA
Essential for protein synthesis
Predominant material in a ribosome (60%)
Catalyzes polypeptide formation (Ribozyme)

mRNA - answer✔Messanger RNA
Conveys genetic info from DNA to ribosome
Specifies the amino acid sequence of protein products

Which type of RNA catalyzes the synthesis of proteins? - answer✔rRNA

What enzyme complex makes DNA from RNA? - answer✔Reverse transcriptase
The first half of DNA restriction site has the sequence AACC, what are the next four bases on
the same chain? - answer✔GGTT

What percentage of human chromosomal DNA codes for proteins? - answer✔1-2%
DNA replication involves the nucleophilic attack on a deoxynucleotide phosphate by a: -
answer✔3' hydroxyl group of deoxyribose
What additional factor does lagging strand DNA synthesis require that is not needed for leading
strand synthesis? - answer✔a DNA ligase

Various forms of DNA that vary in the degree of winding are called: - answer✔Topoisomers


1|Page

, ©FYNDLAY EXAM SOLUTIONS 2024/2025

ALL RIGHTS RESERVED.
If the first four bases are ATGC, what are the next four bases on the SAME DNA strand? -
answer✔GCAT

During DNA replication the lagging DNA strand is READ at its: - answer✔3' end
In addition to hydrogen bonding, the stability of double helix between complementary DNA
chains is promoted by? - answer✔Aromatic stacking
In the structure of ATP the inner phosphate group is joined to the 5' carbon of ribose by what
type of bond? - answer✔Phosphoester

What feature is found in DNA synthesis but NOT in RNA synthesis? - answer✔Repair
mechanisms which remove incorrect bases

Which statement is TRUE about ribosomes? - answer✔Protein synthesis is catalyzed by
ribosomal RNA

The process of cutting DNA strands at specific sites is accomplished by? - answer✔Restriction
endonucleases
Which of the following is needed for replication of DNA but NOT for transcription of DNA to
RNA? - answer✔a primer strand
In bacteria a protein with 1200 amino acids might be coded by a sequence of DNA with an open
reading frame of - answer✔3600 bases (3 for each AA)

Which of the following statements is TRUE about the coding strand of DNA? - answer✔It has
the same base sequence as the transcript except for the substitution of U's
The signals at the ends of introns in immature mRNA, that code for their removal are: -
answer✔GU and AG
If a restriction site in a circular plasmid has the sequence GGAATTCC, and the endonucleas cuts
between A and A, how many unpaired bases will occur on each strand at the opening that is
produced? - answer✔2

In RNA synthesis to which base in the DNA is the U in the RNA product paired? - answer✔A

At neutral pH, DNA carries a negative charge because of the - answer✔Phosphate
The critical ingredient in PCR which allows for multiple rounds of DNA synthesis is? -
answer✔The heat resistant DNA polymerase
The hydrolytic enzymes, used in cloning experiments, which make cuts at distinct sites in DNA
are called? - answer✔restriction endonucleases

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