Howard MIC224 Final Practical With
Solution
What does MSA stand for? - ANSWER mannitol salt agar
What is the substrate in MSA? - ANSWER mannitol
What is the indicatior in MSA? - ANSWER phenol red
What's the selective agent in MSA? - ANSWER NaCl
What color would a positive reaction be on MSA? - ANSWER yellow (s.
aureus)
What color would a negative reaction be on MSA? - ANSWER red (s.
epidermis)
What does GEL stand for? - ANSWER gelatin agar
Is MSA selective or differential? - ANSWER selective
What is the substrate in GEL? - ANSWER gelatin
What is the indicator in GEL? - ANSWER HCl
What is the selective agent in GEL? - ANSWER none
What does a positive reaction (zone) mean in GEL plates? - ANSWER the
organism produces gelatinase
What does STA stand for? - ANSWER starch agar
What is the substrate in STA? - ANSWER starch
, What is the indicator in STA? - ANSWER iodine
What is the selective agent in STA? - ANSWER none
Is STA selective or differential? - ANSWER differential
What does a positive reaction to STA mean? - ANSWER the organism
produces amylase
What does SBA stand for? - ANSWER sheep's blood agar
What is the substrate in SBA? - ANSWER blood
What is the indicator in SBA? - ANSWER none
What is the selective agent in SBA? - ANSWER none
Is SBA selective or differential? - ANSWER differential
What does alpha hemolysis mean? - ANSWER green, incomplete clearing
What does beta hemolysis mean? - ANSWER complete clearing, zone
What does gamma hemolysis mean? - ANSWER no clearing, nothing
What is the selective agent in PEA? - ANSWER phenol alcohol
Solution
What does MSA stand for? - ANSWER mannitol salt agar
What is the substrate in MSA? - ANSWER mannitol
What is the indicatior in MSA? - ANSWER phenol red
What's the selective agent in MSA? - ANSWER NaCl
What color would a positive reaction be on MSA? - ANSWER yellow (s.
aureus)
What color would a negative reaction be on MSA? - ANSWER red (s.
epidermis)
What does GEL stand for? - ANSWER gelatin agar
Is MSA selective or differential? - ANSWER selective
What is the substrate in GEL? - ANSWER gelatin
What is the indicator in GEL? - ANSWER HCl
What is the selective agent in GEL? - ANSWER none
What does a positive reaction (zone) mean in GEL plates? - ANSWER the
organism produces gelatinase
What does STA stand for? - ANSWER starch agar
What is the substrate in STA? - ANSWER starch
, What is the indicator in STA? - ANSWER iodine
What is the selective agent in STA? - ANSWER none
Is STA selective or differential? - ANSWER differential
What does a positive reaction to STA mean? - ANSWER the organism
produces amylase
What does SBA stand for? - ANSWER sheep's blood agar
What is the substrate in SBA? - ANSWER blood
What is the indicator in SBA? - ANSWER none
What is the selective agent in SBA? - ANSWER none
Is SBA selective or differential? - ANSWER differential
What does alpha hemolysis mean? - ANSWER green, incomplete clearing
What does beta hemolysis mean? - ANSWER complete clearing, zone
What does gamma hemolysis mean? - ANSWER no clearing, nothing
What is the selective agent in PEA? - ANSWER phenol alcohol