MIC224 Midterm Test With
Complete Solution
Ocular - ANSWER moves left/right to adjust interpupillary distance
Binocular tube - ANSWER swivels to permit eyepiece housing to rotate.
Stage adjustment knobs - ANSWER used to move the slide on a mechanical
stage in vertical and horizontal directions
Lamp intensity Dial - ANSWER used to adjust light intensity
Revolving nose piece - ANSWER attached to objective lenses and rotates
around
objective lenses - ANSWER magnification that starts at 4x goes to 10x, 40x,
then 100x.
Condenser - ANSWER used to adjust both intensity and contrast of light.
Total magnification - ANSWER Multiply ocular lens (eyepiece) by the
objective lens
resolution - ANSWER is the ability to discern the fine detail. Just making an
image larger does not make the ability to see it better.
Light capturing ability - ANSWER what creates resolution. Microscopes are
specifically manufactured to capture the greatest amount of light even at
high magnifications.
The 100x oil immersion lens - ANSWER is designed to utilize a small drop of
oil that has the same refractive index as glass. The light is "fooled" into
,thinking that it has not left the slide and enters into the lens in a strait line
minimizing loses. You cannot focus this lens without oil.
Ocular scale - ANSWER Identify
interpupillary distance - ANSWER distance between eyepieces
Arm of microscope - ANSWER 9used to pick up the mictoscope
condenser height adjustment knob - ANSWER used to raise and lower
condenser houseing. Adjust light intensity enetering objective lenses. Should
be adjusted so that light does not blind viewer, yet illuminate specimen fully..
coarse focus knob - ANSWER moves stage height in large increments. Used
only for finding and initially focusing on specimen using low power lenses
only.
Fine adjustment knob - ANSWER moves stage height in fine increments. Used
with lower power lenses for final focus. Must be used in 40x and 100x
Ubiquitous - ANSWER found everywhere; microbes are this.
Mircroorganisms - ANSWER too small to look at with the naked eye.
How to look at microorganisms - ANSWER grow the organism and then stain,
use microscope to look at after.
How we grow microorganisms - ANSWER with the use of agar
Agar - ANSWER a gel-like polysaccharide compound used for culturing
microbes; extracted from certain red algae
Solid media - ANSWER made of agar
Liquid media - ANSWER made of broth
, Stab - ANSWER another wat to see solid media. Were solid media is put in a
test tube and then an inoculating needle is stabbed through with the
organism on it. Sometimes call ed a deep.
Slant form - ANSWER agar is placed in a test tub and before it drys, we slant
the tube to give a nice flat surface to place bacteria on. Easier to store.
PCA - ANSWER Plate Count Agar: general purpose media. Used for swapping
desk or show and then placed on media.
PDA - ANSWER Potato dextrose agar. Selective media used to grow yeast
and molds (fungi) - this is the one we left on our desk for the air to
contaminate.
Each group of microorganism on a media - ANSWER not individual cells, but
colonies
Mold and fungi - ANSWER give off hair like projections called filaments
growth. That is the main difference on how to tell them apart.
How to label plate - ANSWER Lid has nothing on it, bottom (where agar is) is
labeled with initials and what it is.
Point of bacterial growth experiment in class - ANSWER to show that
bacteria are everywhere and need to see how not to cross contaminate
How much agar is added to the media for a petri dish? - ANSWER 1.5%
Because bacteria are found everywhere, they are considered to be
______________. - ANSWER ubiquitous
Which media will be used to grow bacteria from swabbing your
surroundings? - ANSWER PCA
Complete Solution
Ocular - ANSWER moves left/right to adjust interpupillary distance
Binocular tube - ANSWER swivels to permit eyepiece housing to rotate.
Stage adjustment knobs - ANSWER used to move the slide on a mechanical
stage in vertical and horizontal directions
Lamp intensity Dial - ANSWER used to adjust light intensity
Revolving nose piece - ANSWER attached to objective lenses and rotates
around
objective lenses - ANSWER magnification that starts at 4x goes to 10x, 40x,
then 100x.
Condenser - ANSWER used to adjust both intensity and contrast of light.
Total magnification - ANSWER Multiply ocular lens (eyepiece) by the
objective lens
resolution - ANSWER is the ability to discern the fine detail. Just making an
image larger does not make the ability to see it better.
Light capturing ability - ANSWER what creates resolution. Microscopes are
specifically manufactured to capture the greatest amount of light even at
high magnifications.
The 100x oil immersion lens - ANSWER is designed to utilize a small drop of
oil that has the same refractive index as glass. The light is "fooled" into
,thinking that it has not left the slide and enters into the lens in a strait line
minimizing loses. You cannot focus this lens without oil.
Ocular scale - ANSWER Identify
interpupillary distance - ANSWER distance between eyepieces
Arm of microscope - ANSWER 9used to pick up the mictoscope
condenser height adjustment knob - ANSWER used to raise and lower
condenser houseing. Adjust light intensity enetering objective lenses. Should
be adjusted so that light does not blind viewer, yet illuminate specimen fully..
coarse focus knob - ANSWER moves stage height in large increments. Used
only for finding and initially focusing on specimen using low power lenses
only.
Fine adjustment knob - ANSWER moves stage height in fine increments. Used
with lower power lenses for final focus. Must be used in 40x and 100x
Ubiquitous - ANSWER found everywhere; microbes are this.
Mircroorganisms - ANSWER too small to look at with the naked eye.
How to look at microorganisms - ANSWER grow the organism and then stain,
use microscope to look at after.
How we grow microorganisms - ANSWER with the use of agar
Agar - ANSWER a gel-like polysaccharide compound used for culturing
microbes; extracted from certain red algae
Solid media - ANSWER made of agar
Liquid media - ANSWER made of broth
, Stab - ANSWER another wat to see solid media. Were solid media is put in a
test tube and then an inoculating needle is stabbed through with the
organism on it. Sometimes call ed a deep.
Slant form - ANSWER agar is placed in a test tub and before it drys, we slant
the tube to give a nice flat surface to place bacteria on. Easier to store.
PCA - ANSWER Plate Count Agar: general purpose media. Used for swapping
desk or show and then placed on media.
PDA - ANSWER Potato dextrose agar. Selective media used to grow yeast
and molds (fungi) - this is the one we left on our desk for the air to
contaminate.
Each group of microorganism on a media - ANSWER not individual cells, but
colonies
Mold and fungi - ANSWER give off hair like projections called filaments
growth. That is the main difference on how to tell them apart.
How to label plate - ANSWER Lid has nothing on it, bottom (where agar is) is
labeled with initials and what it is.
Point of bacterial growth experiment in class - ANSWER to show that
bacteria are everywhere and need to see how not to cross contaminate
How much agar is added to the media for a petri dish? - ANSWER 1.5%
Because bacteria are found everywhere, they are considered to be
______________. - ANSWER ubiquitous
Which media will be used to grow bacteria from swabbing your
surroundings? - ANSWER PCA
