Micro Lab Midterm Exam 2024/2025

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Micro Lab Midterm Exam 2024/2025

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Micro Lab Midterm Exam 2024/2025 Micro Lab Midterm Exam 2024/2025 Micro Lab Midterm Exam 2024/2025

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Micro Lab Midterm Exam
Gram-positive microorganisms have a higher ____________ and lower ______ content than gram-
negative microorganisms. - ANS 1. peptidoglycan

2. lipid

Why are gram negative cells easily decolorized? - ANS because the ethanol dissolves the high lipid cell
wall allowing the crystal violet-iodine complex to readily exit the cell

What color will the gram positive cells be after the primary stain (crystal violet)? - ANS purple

What color will the gram negative cells be after the primary stain (crystal violet)? - ANS purple

What color will the gram positive cells be after the mordant (iodine) is added? - ANS purple

What color will the gram positive cells be after the decolorizer (ethanol) is added? - ANS purple

What color will the gram positive cells be after the counterstain (saffranin)? - ANS purple

What color will the gram negative cells be after the mordant (iodine) is added? - ANS purple

What color will the gram negative cells be after the decolorizer (ethanol) is added? - ANS colorless

What color will the gram negative cells be after the counterstain (saffranin)? - ANS pink

What color will the Acid Fast positive cells be after the primary stain (Carbol Fuschin)? - ANS red

, What color will the Acid Fast positive cells be after the decolorizer (acid alcohol) is added? - ANS red

What color will the Acid Fast positive cells be after the counterstain (Methylene Blue)? - ANS red

What color will the Acid Fast negative cells be after the primary stain (Carbol Fuschin)? - ANS red

What color will the Acid Fast negative cells be after the decolorizer (acid alcohol) is added? - ANS
colorless

What color will the Acid Fast negative cells be after the counterstain (Methylene Blue)? - ANS blue

Two genera of Acid Fast Organisms - ANS Mycobacterium and Nocardia.

What are the two important species of Mycobacterium? - ANS tuberculosis and leprae.

Endospores appear as... - ANS a green center within a red cell

What color will a non spore be after the decolorizer (water)? - ANS colorless

What color will a non spore be after the counterstain (Safranin)? - ANS pink

Cationic Dye - ANS methylene blue

crystal violet

Anionic Dye - ANS acid fuchsin

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Uploaded on: November 1, 2024
Number of pages: 7
Written in: 2024/2025
Type: Exam (elaborations)
Contains: Questions & answers

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