BMD 321 Exam 3 Study
Guide
T/F: RNA is less stable to alkaline hydrolysis than DNA because RNA's 2'-OH group
makes the 3'-phosphodiester bond susceptible to nucleophilic chemical attack. -
Answer True
Which statement about DNA is NOT true?
a) The two strands are held together by hydrogen bonds.
b) The phosphate is on the outside of the helix.
c) The two strands have complementary base pairing.
d) The polarity of the two strands is parallel.
e) Bases are on the interior of the DNA. - Answer d) The polarity of the two strands is
parallel.
T/F: The higher the GC content of a DNA, the lower the melting temperature. - Answer
False
Which statement is NOT true?
a) DNA binds histones through electrostatic interactions between positively charged
amino acids of histones with the negative charged phosphate backbone of the DNA.
b) Histones are made up of duplicate copies of H2A, H2B, H3, H4.
c) In the electron microscope, nucleosomes look like "beads on a string."
d) Chromatin is made up of DNA and proteins.
e) Histones are found in prokaryotes and eukaryotes. - Answer e) Histones are found in
prokaryotes and eukaryotes.
Removal of the RNA primer and replacement with DNA is carried out by:
a) DNA polymerase I
b) Topoisomerase II
c) DNA polymerase III
d) Primase
, e) Ligase - Answer a) DNA polymerase I
Which is the first protein involved in DNA replication?
a) DNA B
b) Ligase
c) DNA polymerase I
d) DNA A
e) Topoisomerase I - Answer e) Topoisomerase I
Which is/are the components used in DNA replication?
a) Nucleotides
b) DNA template
c) DNA polymerase
d) RNA primer
e) All of the components are required. - Answer e) All of the components are required.
Which is NOT found in DNA?
a) Cytosine
b) Adenine
c) Thymine
d) Guanine
e) Uracil - Answer e) Uracil
What statement is NOT true?
a) DNA polymerase I is involved in filling in the gaps of the removed primer.
b) DNA polymerase I and III have replication function.
c) DNA polymerase III adds more nucleotides than DNA polymerase I.
d) DNA polymerase I is more processive than DNA polymerase III.
e) DNA polymerase III is involved in the leading strand. - Answer d) DNA polymerase I is
more processive than DNA polymerase III.
The enzyme responsible for forming the phosphodiester bond between two DNA
fragments during replication is:
Guide
T/F: RNA is less stable to alkaline hydrolysis than DNA because RNA's 2'-OH group
makes the 3'-phosphodiester bond susceptible to nucleophilic chemical attack. -
Answer True
Which statement about DNA is NOT true?
a) The two strands are held together by hydrogen bonds.
b) The phosphate is on the outside of the helix.
c) The two strands have complementary base pairing.
d) The polarity of the two strands is parallel.
e) Bases are on the interior of the DNA. - Answer d) The polarity of the two strands is
parallel.
T/F: The higher the GC content of a DNA, the lower the melting temperature. - Answer
False
Which statement is NOT true?
a) DNA binds histones through electrostatic interactions between positively charged
amino acids of histones with the negative charged phosphate backbone of the DNA.
b) Histones are made up of duplicate copies of H2A, H2B, H3, H4.
c) In the electron microscope, nucleosomes look like "beads on a string."
d) Chromatin is made up of DNA and proteins.
e) Histones are found in prokaryotes and eukaryotes. - Answer e) Histones are found in
prokaryotes and eukaryotes.
Removal of the RNA primer and replacement with DNA is carried out by:
a) DNA polymerase I
b) Topoisomerase II
c) DNA polymerase III
d) Primase
, e) Ligase - Answer a) DNA polymerase I
Which is the first protein involved in DNA replication?
a) DNA B
b) Ligase
c) DNA polymerase I
d) DNA A
e) Topoisomerase I - Answer e) Topoisomerase I
Which is/are the components used in DNA replication?
a) Nucleotides
b) DNA template
c) DNA polymerase
d) RNA primer
e) All of the components are required. - Answer e) All of the components are required.
Which is NOT found in DNA?
a) Cytosine
b) Adenine
c) Thymine
d) Guanine
e) Uracil - Answer e) Uracil
What statement is NOT true?
a) DNA polymerase I is involved in filling in the gaps of the removed primer.
b) DNA polymerase I and III have replication function.
c) DNA polymerase III adds more nucleotides than DNA polymerase I.
d) DNA polymerase I is more processive than DNA polymerase III.
e) DNA polymerase III is involved in the leading strand. - Answer d) DNA polymerase I is
more processive than DNA polymerase III.
The enzyme responsible for forming the phosphodiester bond between two DNA
fragments during replication is:
