BMD 321 Exam Latest Update
cleavage of polypeptides:
1. cyanogen bromide ->
2. trypsin ->
3. chymotrypsin -> - Answer 1. methionine
2. lysine and arginine
3. tyrosine, tryptophan, phenylalanine
oxygen-binding in myoglobin happens at the ______ coordiation site - Answer 6th
coordination site
Myoglobin and hemoglobin bind oxygen in _________.
The heme group consists of an organic component called _____ and acentral iron ion in
the ferrous (Fe2+) form.
The iron lies in the middle of the protoporphyrin bound to __________.
Iron can form two additional bonds, called the _________________________.
The fifth coordination site is occupied by an imidazole ring of a __________ called the
______________.
The sixth coordination site binds ________.
Upon oxygen binding, the iron moves into the plane of the __________________. - Answer
heme groups
protoporphyrin
four nitrogens
fifth and sixth coordination sites
histidine, proximal histidine
oxygen
protoporphryin ring
pO_2 in the lung is
pO_2 in the tissue is - Answer 100 torr
20 torr
, The binding of oxygen to myoglobin follows a ________ curve
The binding of oxygen to hemoglobin follows a _______ curve
Myoglobin curve is _________ hemoglobin curve - Answer hyperbolic (top)
sigmoidal (bottom)
above
2,3 BBG is __________ charges and binds to _________ charged _____________ - Answer
negatively
positively
amino acids
2,3 BBG binds to the _______ form of ____________ - Answer T
hemoglobin
α2Υ2 : Fetus Hb (__________)
α2β2 : Adult Hb (__________)
the γ has a Ser instead of His residue which ________ the affinity of 2, 3-BPG for Hb.
Therefore, oxygen binds more tightly to the _________ Hb, so the ________ state is
stabilized - Answer top
bottom
reduces
fetal
R
At high pH (7.4), the His is not protonated and it does not form salt bridges with Asp.
This does not stabilize the deoxy Hb. But pHof 7.2 causes the protonation of His and salt
bridges form between His and Asp. This stabilizes the ______ (deoxy state) of Hb.
Therefore, _______ oxygen is released at ______ pH (Hb has less affinity for oxygen) -
Answer T state
more
less
_________ stabilizes the T state of Hb - Answer carbamate
R form is stabilized by:
T form is stabilized by: - Answer oxygen binding to heme
cleavage of polypeptides:
1. cyanogen bromide ->
2. trypsin ->
3. chymotrypsin -> - Answer 1. methionine
2. lysine and arginine
3. tyrosine, tryptophan, phenylalanine
oxygen-binding in myoglobin happens at the ______ coordiation site - Answer 6th
coordination site
Myoglobin and hemoglobin bind oxygen in _________.
The heme group consists of an organic component called _____ and acentral iron ion in
the ferrous (Fe2+) form.
The iron lies in the middle of the protoporphyrin bound to __________.
Iron can form two additional bonds, called the _________________________.
The fifth coordination site is occupied by an imidazole ring of a __________ called the
______________.
The sixth coordination site binds ________.
Upon oxygen binding, the iron moves into the plane of the __________________. - Answer
heme groups
protoporphyrin
four nitrogens
fifth and sixth coordination sites
histidine, proximal histidine
oxygen
protoporphryin ring
pO_2 in the lung is
pO_2 in the tissue is - Answer 100 torr
20 torr
, The binding of oxygen to myoglobin follows a ________ curve
The binding of oxygen to hemoglobin follows a _______ curve
Myoglobin curve is _________ hemoglobin curve - Answer hyperbolic (top)
sigmoidal (bottom)
above
2,3 BBG is __________ charges and binds to _________ charged _____________ - Answer
negatively
positively
amino acids
2,3 BBG binds to the _______ form of ____________ - Answer T
hemoglobin
α2Υ2 : Fetus Hb (__________)
α2β2 : Adult Hb (__________)
the γ has a Ser instead of His residue which ________ the affinity of 2, 3-BPG for Hb.
Therefore, oxygen binds more tightly to the _________ Hb, so the ________ state is
stabilized - Answer top
bottom
reduces
fetal
R
At high pH (7.4), the His is not protonated and it does not form salt bridges with Asp.
This does not stabilize the deoxy Hb. But pHof 7.2 causes the protonation of His and salt
bridges form between His and Asp. This stabilizes the ______ (deoxy state) of Hb.
Therefore, _______ oxygen is released at ______ pH (Hb has less affinity for oxygen) -
Answer T state
more
less
_________ stabilizes the T state of Hb - Answer carbamate
R form is stabilized by:
T form is stabilized by: - Answer oxygen binding to heme
