Medical Interventions EOC exam with
complete solutions
Microbe that cause influenza were killed and used to develop a new vaccine. The
Killed cells contain recognizable antigens on their surface that can generate an
immune response. However, they cannot cause disease. To which class of vaccines
does the new vaccine belong? - 🔷ANSWERS 🖊✔✔Inactivated whole agent
Why must DNA be heated to 94 degrees C during the first step in the polymerase
chain reaction cycle? - 🔷ANSWERS 🖊✔✔to break hydrogen bonds between DNA
strands
Beta-lactam antibiotics, such as penicillin, inhibit bacterial enzymes involved in.. -
🔷ANSWERS 🖊✔✔breaking down the peptidoglycan cell wall
You are in charge of quality control at a biomanufactoring company and need to hire
a person to verify the purity of a manufactured protein. Which is the most important
skill that a person you would hire would possess? - 🔷ANSWERS 🖊✔✔Experience
running and analyzing results of protein ekectrophoresis
Sensorineural hearing loss is caused by.... - 🔷ANSWERS 🖊✔✔damage to the
auditory nerve
hydrophobic interactive chromatography is a method used to purify a protein based
on.. - 🔷ANSWERS 🖊✔✔it's hydrophobic properties
Cochlear implants could possibly help a person who has a problem with their... -
🔷ANSWERS 🖊✔✔inner ear
the parts of the ear that converts stimulus from the outside environment into the
nerve impulses for transmission to the brain is the... - 🔷ANSWERS 🖊✔✔cochlea
,DNA ligase is - 🔷ANSWERS 🖊✔✔A linking enzyme that links pieces of DNA
together
Recombinant DNA can be inserted into a bacteria using a bacteriophage through the
process of... - 🔷ANSWERS 🖊✔✔transduction
Epidemiology is the ... - 🔷ANSWERS 🖊✔✔study of bacterial infections and how
they are transferred in a population
A bacterial culture is several days old. Many of the bacterial cells have ruptured and
released DNA into the nutrient agar. A few living bacteria cells in the culture have
become genetically enhanced by taking the DNA into the cytoplasm and are now
resistant to amplicillin. The above explains which of the following processes? -
🔷ANSWERS 🖊✔✔Transformation
During the ELISA test the secondary antibody was conjugated with an enzyme.
What happens when that enzyme was attached with a substrate? - 🔷ANSWERS 🖊
✔✔the substrate causes the enzyme to oxidize resulting in color change
A tumor suppressor gene is a gene that.. - 🔷ANSWERS 🖊✔✔produces proteins that
inhibit cell division
DNA micro-arrays measure - 🔷ANSWERS 🖊✔✔the amount of cDNA for every gene
that is expressed
Which of the following antibiotics blocks the attachment of tRNA to the ribosome
preventing protein synthesis - 🔷ANSWERS 🖊✔✔tetracylines
What bio-medical professionals are involved in the stages of producing and
manufacturing a protein product - 🔷ANSWERS 🖊✔✔genetic engineers isolate and
insert gene
microbiologists culture bacteria
lab techs generate/purify protein
pharmacologists design/test medications
, What role does insulin play in diabetes? - 🔷ANSWERS 🖊✔✔INSULIN allows cells to
take up sugar, getting sugar out of the blood
Type 1 diabetics need insulin injections to survive—otherwise their cells will die
(need glucose to make energy
How has the diagnosis and treatment for diabetes changed in the last 200 years? -
🔷ANSWERS 🖊✔✔Long ago—diagnosed by tasting pee for sweetness
1700's-1800's—treated with dietary restrictions & deadly chemicals
1920s on—insulin used to treat patients
How can bacterial plasmids be used to produce proteins such as insulin? -
🔷ANSWERS 🖊✔✔This is done through genetic engineering
Plasmids are isolated from bacteria
Plasmid is cut with restriction enzyme
The gene for insulin (or other protein) is inserted into the plasmid
Plasmid is closed (it now contains recombinant DNA—DNA from multiple sources)
Bacteria are made to take up plasmid and pump out protein
What is bacterial transformation? - 🔷ANSWERS 🖊✔✔Transformation is the process
by which cells are made to take up a genetically engineered plasmid
after that they'll make the protein that the inserted gene(s) code for
Transformed bacteria are grown on antibiotic like ampicillin to ensure purity of
culture
How can you gauge the success of a transformation experiment? - 🔷ANSWERS 🖊
✔✔You have to calculate the transformation efficiency, which how many
transformants there were per microgram of DNA added
How does amino acid structure relate to the overall shape of a protein? -
🔷ANSWERS 🖊✔✔Proteins are made of amino acids, some of which are hydrophilic
(water-loving) and some of which are hydrophobic (water-fearing)
In an aqueous environment (bottom right), proteins fold so that the hydrophobic
amino acids "hide" on the inside
complete solutions
Microbe that cause influenza were killed and used to develop a new vaccine. The
Killed cells contain recognizable antigens on their surface that can generate an
immune response. However, they cannot cause disease. To which class of vaccines
does the new vaccine belong? - 🔷ANSWERS 🖊✔✔Inactivated whole agent
Why must DNA be heated to 94 degrees C during the first step in the polymerase
chain reaction cycle? - 🔷ANSWERS 🖊✔✔to break hydrogen bonds between DNA
strands
Beta-lactam antibiotics, such as penicillin, inhibit bacterial enzymes involved in.. -
🔷ANSWERS 🖊✔✔breaking down the peptidoglycan cell wall
You are in charge of quality control at a biomanufactoring company and need to hire
a person to verify the purity of a manufactured protein. Which is the most important
skill that a person you would hire would possess? - 🔷ANSWERS 🖊✔✔Experience
running and analyzing results of protein ekectrophoresis
Sensorineural hearing loss is caused by.... - 🔷ANSWERS 🖊✔✔damage to the
auditory nerve
hydrophobic interactive chromatography is a method used to purify a protein based
on.. - 🔷ANSWERS 🖊✔✔it's hydrophobic properties
Cochlear implants could possibly help a person who has a problem with their... -
🔷ANSWERS 🖊✔✔inner ear
the parts of the ear that converts stimulus from the outside environment into the
nerve impulses for transmission to the brain is the... - 🔷ANSWERS 🖊✔✔cochlea
,DNA ligase is - 🔷ANSWERS 🖊✔✔A linking enzyme that links pieces of DNA
together
Recombinant DNA can be inserted into a bacteria using a bacteriophage through the
process of... - 🔷ANSWERS 🖊✔✔transduction
Epidemiology is the ... - 🔷ANSWERS 🖊✔✔study of bacterial infections and how
they are transferred in a population
A bacterial culture is several days old. Many of the bacterial cells have ruptured and
released DNA into the nutrient agar. A few living bacteria cells in the culture have
become genetically enhanced by taking the DNA into the cytoplasm and are now
resistant to amplicillin. The above explains which of the following processes? -
🔷ANSWERS 🖊✔✔Transformation
During the ELISA test the secondary antibody was conjugated with an enzyme.
What happens when that enzyme was attached with a substrate? - 🔷ANSWERS 🖊
✔✔the substrate causes the enzyme to oxidize resulting in color change
A tumor suppressor gene is a gene that.. - 🔷ANSWERS 🖊✔✔produces proteins that
inhibit cell division
DNA micro-arrays measure - 🔷ANSWERS 🖊✔✔the amount of cDNA for every gene
that is expressed
Which of the following antibiotics blocks the attachment of tRNA to the ribosome
preventing protein synthesis - 🔷ANSWERS 🖊✔✔tetracylines
What bio-medical professionals are involved in the stages of producing and
manufacturing a protein product - 🔷ANSWERS 🖊✔✔genetic engineers isolate and
insert gene
microbiologists culture bacteria
lab techs generate/purify protein
pharmacologists design/test medications
, What role does insulin play in diabetes? - 🔷ANSWERS 🖊✔✔INSULIN allows cells to
take up sugar, getting sugar out of the blood
Type 1 diabetics need insulin injections to survive—otherwise their cells will die
(need glucose to make energy
How has the diagnosis and treatment for diabetes changed in the last 200 years? -
🔷ANSWERS 🖊✔✔Long ago—diagnosed by tasting pee for sweetness
1700's-1800's—treated with dietary restrictions & deadly chemicals
1920s on—insulin used to treat patients
How can bacterial plasmids be used to produce proteins such as insulin? -
🔷ANSWERS 🖊✔✔This is done through genetic engineering
Plasmids are isolated from bacteria
Plasmid is cut with restriction enzyme
The gene for insulin (or other protein) is inserted into the plasmid
Plasmid is closed (it now contains recombinant DNA—DNA from multiple sources)
Bacteria are made to take up plasmid and pump out protein
What is bacterial transformation? - 🔷ANSWERS 🖊✔✔Transformation is the process
by which cells are made to take up a genetically engineered plasmid
after that they'll make the protein that the inserted gene(s) code for
Transformed bacteria are grown on antibiotic like ampicillin to ensure purity of
culture
How can you gauge the success of a transformation experiment? - 🔷ANSWERS 🖊
✔✔You have to calculate the transformation efficiency, which how many
transformants there were per microgram of DNA added
How does amino acid structure relate to the overall shape of a protein? -
🔷ANSWERS 🖊✔✔Proteins are made of amino acids, some of which are hydrophilic
(water-loving) and some of which are hydrophobic (water-fearing)
In an aqueous environment (bottom right), proteins fold so that the hydrophobic
amino acids "hide" on the inside
