MICRO FOR NURSING- MIDTERM EXAM. MIDTERM EXAM
REVISION QUESTIONS AND CORRECT ANSWERS (GRADED A+)
(100% GUARANTEE) (2024 UPDATE)
Where do used plates and tubes go after you use them? - ANSWER- in the hazardous materials
bucket in the front of the lab
Where do used slides go? - ANSWER- in the 'cake pan' in the middle of the lab
How do you transfer bacteria from plate or tube to slides, tubes, or plates? - ANSWER- with a
loop
What do you do with the test tube lid when doing a transfer? - ANSWER- hold it in your pinky
Pure culture - ANSWER- one type of bacteria present
Contaminated - ANSWER- unwanted colonies mixed in with your bacteria
Using a T streak - ANSWER- the idea is to thin the bacteria so individual colonies are apparent,
allowing you to see if you have a pure culture or not. You burn your loop between zones to thin
the bacteria out to allow individual colonies to grow
Basic stain - ANSWER- positively charged dye molecules
Acidic stain - ANSWER- negatively charged dye molecules
Type of dye used in the negative stain and what we substituted with - ANSWER- nigrosine,
and we substituted it with a permanent marker
REVISION QUESTIONS AND CORRECT ANSWERS (GRADED A+)
(100% GUARANTEE) (2024 UPDATE)
Where do used plates and tubes go after you use them? - ANSWER- in the hazardous materials
bucket in the front of the lab
Where do used slides go? - ANSWER- in the 'cake pan' in the middle of the lab
How do you transfer bacteria from plate or tube to slides, tubes, or plates? - ANSWER- with a
loop
What do you do with the test tube lid when doing a transfer? - ANSWER- hold it in your pinky
Pure culture - ANSWER- one type of bacteria present
Contaminated - ANSWER- unwanted colonies mixed in with your bacteria
Using a T streak - ANSWER- the idea is to thin the bacteria so individual colonies are apparent,
allowing you to see if you have a pure culture or not. You burn your loop between zones to thin
the bacteria out to allow individual colonies to grow
Basic stain - ANSWER- positively charged dye molecules
Acidic stain - ANSWER- negatively charged dye molecules
Type of dye used in the negative stain and what we substituted with - ANSWER- nigrosine,
and we substituted it with a permanent marker
