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BIO 1404 UTSA Lab practical 2

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BIO 1404 UTSA Lab practical 2

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reproduction between _ ___individuals, called a ___ cross, results in dominant&recessive
phenotypes among the offspring, expected ___ ratio of _:_.




2 heterozygous - monohybrid - phenotypic - 3:1

,____ analysis for ___




RFLP - paternity




After a day, a ___ emerges ___ the ___. larva goes through three stages called ___. When
the third instar ends, a ___is formed. At end, the ___ emerges from casing.




larva - from - egg - instars - pupa - adult




___ digests are used to determine ___ ___ ___ are generated __ a particular ___ enzyme, ___
___ are used to order&___ the ___ correctly.




Single - how many fragments - by - restriction - double digests - orient -
fragments




restriction ___ characterize&___ ___molecules. they're enzymes that recognize a ___ DNA
nucleotide ___&___the DNA at or near this site. Many of these sites are ___; that is, the ___
of the ___ strands ___ the ___ nucleotide ___ backward and forward.

, endonucleases - manipulate DNA - specific - sequence - cut -
palindromes - sequences - complementary - read - same - sequence




___ occurs in the ___ ___ to produce ___ (sperm&eggs). requires _ divisions producing four
___ gametes that have ___ the number of ___ as the parental cells. ___ of the gametes ___
the species ___ number of chromosomes.




meiosis - germ - cells - gametes - 2 - haploid - half - chromosomes -
fertilization - restores -diploid




The somatic tissues of an organism grow & repair by producing new cells by ___. the
new daughter cells receive ___ ___ number of chromosomes as the parental cell




mitosis - the same -




During agarose gel-___, ___ ___molecules ___ through a gel bc they are placed in a
powerful ___ ___. ___ causes DNA fragments, which are ___ charged, to migrate ___ the
positive ___. since the ___ is directly ___ to the ___ of the molecule, the fragments will
separate according to their size. ___ fragments will move more ___, ___fragments more
___. The fragments will be detectable when the gel is illuminated with a light box
because the ___ contains a ___ acid ___, which ___ ___ and is fluorescent




electrophoresis - charged DNA - migrate - electrical field - electricity -
negatively - toward - electrode - charge - proportional - size - Small -

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