Automation of Blood Banking Test Exam Solution Manual 100% Pass
Why was Gel Technology made by Dr. Yves Lapierre in 1988? - Answers Minimize problems associated
with conventional techniques of blood grouping
What is the principle of gel technology? - Answers controlled centrifugation of RBCs through gel columns
(micro tubes) where serum and cell reaction takes place.
Describe a microtube - Answers reaction chamber that narrows to become a column with a conical
bottom, is designed to allow prior incubation of test serum and RBCs
What does each gel column contain? - Answers Sephacryl gel suspended in a buffer solution
Describe Gel Technology Procedure? - Answers Microtubes filled with acrylamide gel in conjunction with
LISS suspended screening cells, antisera is pre-added to tubes for antigen typing or ABO/RH
Describe a 4+ gel reaction - Answers Solid band of red cells at top of gel
Describe a 3+ gel reaction - Answers Agglutinated red cells in upper half
Describe a 2+ gel reaction - Answers Red cell agglutinates through length
Describe a 1+ gel reaction - Answers Agglutinated red cells in lower half of gel column
Describe a negative gel reaction - Answers No cell agglutination all at bottom
What are the uses for Gel Technology? - Answers Any test that has haemagglutination as its end point.
Give some examples of tests done with Gel Technology - Answers ABO-Rh typing, Antibody Type and
Screen, Compatibility Testing, DAT/IAT, Antibody classification, PNH, Sickle Cell Anemia testing
What are the advantages of Gel Technology? - Answers Improved sensitivity, specificity, easy to
use/read, no wash phase in IAT, minimal training, gives reliable results, easy storage/shelf life, widest
range of reagents and instrumentation
What are the disadvantages of Gel Technology? - Answers Needs special centrifuge to accommodate
micro tube cards, special incubators, pipette to dispense 25 microliters of serum, 50 microliters of RBC
suspension, is expensive, needs skilled workers
What isSolid Phase Red Cell Adherence Assay? - Answers LISS, microtiter well method where RBC
antigen is coated on bottom of well, serum/plasma and LISS are added to well following incubation
where IgG coated indicator is added
What is considered a positive reaction in Solid Phase Technology? - Answers Anti-globulin coated
indicator binds to anti-body coated wells forming an intact layer or cells on the well surface
What is considered a negative reaction in Solid Phase Technology? - Answers Button of indicator red
cells at the bottom of test wells shows no area of adherence
Why was Gel Technology made by Dr. Yves Lapierre in 1988? - Answers Minimize problems associated
with conventional techniques of blood grouping
What is the principle of gel technology? - Answers controlled centrifugation of RBCs through gel columns
(micro tubes) where serum and cell reaction takes place.
Describe a microtube - Answers reaction chamber that narrows to become a column with a conical
bottom, is designed to allow prior incubation of test serum and RBCs
What does each gel column contain? - Answers Sephacryl gel suspended in a buffer solution
Describe Gel Technology Procedure? - Answers Microtubes filled with acrylamide gel in conjunction with
LISS suspended screening cells, antisera is pre-added to tubes for antigen typing or ABO/RH
Describe a 4+ gel reaction - Answers Solid band of red cells at top of gel
Describe a 3+ gel reaction - Answers Agglutinated red cells in upper half
Describe a 2+ gel reaction - Answers Red cell agglutinates through length
Describe a 1+ gel reaction - Answers Agglutinated red cells in lower half of gel column
Describe a negative gel reaction - Answers No cell agglutination all at bottom
What are the uses for Gel Technology? - Answers Any test that has haemagglutination as its end point.
Give some examples of tests done with Gel Technology - Answers ABO-Rh typing, Antibody Type and
Screen, Compatibility Testing, DAT/IAT, Antibody classification, PNH, Sickle Cell Anemia testing
What are the advantages of Gel Technology? - Answers Improved sensitivity, specificity, easy to
use/read, no wash phase in IAT, minimal training, gives reliable results, easy storage/shelf life, widest
range of reagents and instrumentation
What are the disadvantages of Gel Technology? - Answers Needs special centrifuge to accommodate
micro tube cards, special incubators, pipette to dispense 25 microliters of serum, 50 microliters of RBC
suspension, is expensive, needs skilled workers
What isSolid Phase Red Cell Adherence Assay? - Answers LISS, microtiter well method where RBC
antigen is coated on bottom of well, serum/plasma and LISS are added to well following incubation
where IgG coated indicator is added
What is considered a positive reaction in Solid Phase Technology? - Answers Anti-globulin coated
indicator binds to anti-body coated wells forming an intact layer or cells on the well surface
What is considered a negative reaction in Solid Phase Technology? - Answers Button of indicator red
cells at the bottom of test wells shows no area of adherence
