PCB 3063 Exam Latest
Update
Whereas the nucleotide strand used for transcription is termed the
___________________
,
the nontranscribed
strand is called the
_______________ - Answer template strand; nontemplate strand
In transcription, nucleotides are always added to the
___________
end of the
elongating strand. - Answer 3'
In eukaryotes, RNA polymerase
________
transcribes the genes that encode
proteins. - Answer RNA Pol II
An in vitro transcription system that contains a bacterial gene initiates transcription, but
from random points on the DNA. Which protein is most likely missing from the reaction?
- Answer Sigma Factor
What is the difference between the core promoter and
the regulatory promoter in eukaryotic gene transcription? - Answer The core promoter
encompasses the site of initiation and extends either upstream or downstream for an
additional ∼35 nt.
The regulatory sequences include the promoter region together with enhancer
elements.
If the following DNA strand was used as a
, template, what would the sequence of an
mRNA be?
5ʹ GTACCGTC 3ʹ - Answer 3' CAUGGCAG '5 (mRNA molecule)
An in vitro transcription system that contains a bacterial gene
does not
initiate transcription. What is one possible problem? - Answer There is a mutation at -10,
where a promoter consensus sequence is located.
Life requires two basic functions - Answer 1. To store & transmit genetic info (DNA)
2. Ability to catalyze chemical transformations (proteins/enzymes)
Three basic RNA's - Answer 1. mRNA
2. tRNA
3. rRNA
(found in both prokaryotes & eukaryotes)
RNA & DNA differences - Answer DNA: role is to make proteins, double stranded,
deoxyribose sugar, takes place in the nucleus, 1 type of DNA, thymine
RNA: role is to transfer into protein production, single stranded, ribose sugar, takes
place in the nucleus & the cytoplasm, 3 main types of RNA, uracil
RNA & DNA similarities - Answer Have nucleic acids, 5 carbon sugars, phosphate
group, nitrogenous bases
How to make a ribosome? where is it found? - Answer rRNA + protein = rRNA';
cytoplasm
mRNA - Answer Carries the DNA info (code) to the ribosome. In the nucleus & the
cytoplasm of eukaryotes.
Pre-mRNA's are in - Answer Eukaryotes
In bacteria, when do transcription & translation happen? - Answer At the same time.
tRNA - Answer Transfers amino acids to growing peptide chain
snRNA (snurps) - Answer Small Nuclear RNA
similar to ribosomes but smaller, participate in processing pre-mRNA's to mRNA's
snoRNA - Answer Small Nucleolar RNA
Update
Whereas the nucleotide strand used for transcription is termed the
___________________
,
the nontranscribed
strand is called the
_______________ - Answer template strand; nontemplate strand
In transcription, nucleotides are always added to the
___________
end of the
elongating strand. - Answer 3'
In eukaryotes, RNA polymerase
________
transcribes the genes that encode
proteins. - Answer RNA Pol II
An in vitro transcription system that contains a bacterial gene initiates transcription, but
from random points on the DNA. Which protein is most likely missing from the reaction?
- Answer Sigma Factor
What is the difference between the core promoter and
the regulatory promoter in eukaryotic gene transcription? - Answer The core promoter
encompasses the site of initiation and extends either upstream or downstream for an
additional ∼35 nt.
The regulatory sequences include the promoter region together with enhancer
elements.
If the following DNA strand was used as a
, template, what would the sequence of an
mRNA be?
5ʹ GTACCGTC 3ʹ - Answer 3' CAUGGCAG '5 (mRNA molecule)
An in vitro transcription system that contains a bacterial gene
does not
initiate transcription. What is one possible problem? - Answer There is a mutation at -10,
where a promoter consensus sequence is located.
Life requires two basic functions - Answer 1. To store & transmit genetic info (DNA)
2. Ability to catalyze chemical transformations (proteins/enzymes)
Three basic RNA's - Answer 1. mRNA
2. tRNA
3. rRNA
(found in both prokaryotes & eukaryotes)
RNA & DNA differences - Answer DNA: role is to make proteins, double stranded,
deoxyribose sugar, takes place in the nucleus, 1 type of DNA, thymine
RNA: role is to transfer into protein production, single stranded, ribose sugar, takes
place in the nucleus & the cytoplasm, 3 main types of RNA, uracil
RNA & DNA similarities - Answer Have nucleic acids, 5 carbon sugars, phosphate
group, nitrogenous bases
How to make a ribosome? where is it found? - Answer rRNA + protein = rRNA';
cytoplasm
mRNA - Answer Carries the DNA info (code) to the ribosome. In the nucleus & the
cytoplasm of eukaryotes.
Pre-mRNA's are in - Answer Eukaryotes
In bacteria, when do transcription & translation happen? - Answer At the same time.
tRNA - Answer Transfers amino acids to growing peptide chain
snRNA (snurps) - Answer Small Nuclear RNA
similar to ribosomes but smaller, participate in processing pre-mRNA's to mRNA's
snoRNA - Answer Small Nucleolar RNA
