BIOS 280 FINAL EXAM 100% SOLVED
glucose mineral salt agar Correct Ans-simple, defined medium. To survive, organism needs
to make all their cellular components from glucose and inorganic salts
trypticase soy agar Correct Ans-undefined medium with soy products and proteins.
Supports growth for most bacteria, including those that require vitamins, pre-formed amino
acids, or other growth factors
blood agar Correct Ans-very rich medium, 5% sheep's blood is added to the agar before it
is poured into the Petri dish. This agar allows evaluation of the type of hemolysis produced by
microorganisms
mannitol salts agar Correct Ans-selective for salt tolerant microorganisms and inhibits the
growth of gram-negative bacteria. It is differential for organisms that ferment mannitol. The
red agar will turn yellow if the organism can ferment mannitol
eosin methylene blue Correct Ans-usually selective for gram-negative rods and usually
inhibits the growth of gram-positive bacteria. It is differential for bacteria that can ferment
lactose. The fermenters will produce dark purple colonies. If the fermenter produces a lot of
, BIOS 280 FINAL EXAM 100% SOLVED
acid as a waste product, the colony will have a green sheen. Non-fermenter colonies will be
white or light pink
gram positive Correct Ans-will appear purple after a gram stain
gram negative Correct Ans-will appear pink/red after a gram stain
size Correct Ans-pinpoint, pinhead, small, medium, large
shape Correct Ans-round or irregular
color Correct Ans-beige, cream, yellow, red, pink, orange
margins Correct Ans-smooth, indistinct, lobulated, lobate, irregular, filamentous
consistency Correct Ans-dry (surface is dull), moist (surface is shiny), mucoid (surface is
slimy)
glucose mineral salt agar Correct Ans-simple, defined medium. To survive, organism needs
to make all their cellular components from glucose and inorganic salts
trypticase soy agar Correct Ans-undefined medium with soy products and proteins.
Supports growth for most bacteria, including those that require vitamins, pre-formed amino
acids, or other growth factors
blood agar Correct Ans-very rich medium, 5% sheep's blood is added to the agar before it
is poured into the Petri dish. This agar allows evaluation of the type of hemolysis produced by
microorganisms
mannitol salts agar Correct Ans-selective for salt tolerant microorganisms and inhibits the
growth of gram-negative bacteria. It is differential for organisms that ferment mannitol. The
red agar will turn yellow if the organism can ferment mannitol
eosin methylene blue Correct Ans-usually selective for gram-negative rods and usually
inhibits the growth of gram-positive bacteria. It is differential for bacteria that can ferment
lactose. The fermenters will produce dark purple colonies. If the fermenter produces a lot of
, BIOS 280 FINAL EXAM 100% SOLVED
acid as a waste product, the colony will have a green sheen. Non-fermenter colonies will be
white or light pink
gram positive Correct Ans-will appear purple after a gram stain
gram negative Correct Ans-will appear pink/red after a gram stain
size Correct Ans-pinpoint, pinhead, small, medium, large
shape Correct Ans-round or irregular
color Correct Ans-beige, cream, yellow, red, pink, orange
margins Correct Ans-smooth, indistinct, lobulated, lobate, irregular, filamentous
consistency Correct Ans-dry (surface is dull), moist (surface is shiny), mucoid (surface is
slimy)
