BIOS 280 lab final; Questions and Answers 100% Correct
transformation Correct Ans-when the streptomycin sensitive acinetobacter cells were able
to grow on the TSA+streptomycin plate when DNA was added
KOH test Correct Ans-gram positive vs. gram negative: KOH on slide, breaks down
peptidoglycan in gram negative= string
catalase test Correct Ans-detects presence of catalase using hydrogen peroxide, which is
broken down into H2O and O2= bubbles meaning catalos positive
oxidase test Correct Ans-detects presence of cytochrome (gram negative)--> use tooth pick
on BBC dry slide, blue= positive
kirby-bauer Correct Ans-measure susceptibility of bacteria to certain antibiotics:
lawn culture on mueller-hinton agar= measure ZOI
colilert test Correct Ans-identifies presence of coliforms/ e. coli:
yellow PSB tube= coliforms present/ beta-galactosidase (ONPG=substrate)
, BIOS 280 lab final; Questions and Answers 100% Correct
blue fluorescent= e. coli/ glutoronidase (MUG= substrate)
streak plate Correct Ans-used to isolate individual colonies, goal =10-20
spread plate Correct Ans-used when counting colonies from dilution series (used hockey
stick to spread), used to calculate the OCD, based off colony #
lawn culture Correct Ans-used for antibiotic testing, even growth across entire plate (1st
section, turn 90, turn 45)
GasPak jar Correct Ans-creates an anaerobic atmosphere: hydrogen gas combines with
oxygen (boiling) removes all oxygen
*indicators for working= indicator strip turns white or presence of oxygen
candle jar Correct Ans-creates an atmosphere with lots of CO2 and little oxygen: ideal
environment for respiratory organisms b/c some bacteria like increased CO2 and some
hemolycins don't tolerate oxygen (which is what we're growing)
transformation Correct Ans-when the streptomycin sensitive acinetobacter cells were able
to grow on the TSA+streptomycin plate when DNA was added
KOH test Correct Ans-gram positive vs. gram negative: KOH on slide, breaks down
peptidoglycan in gram negative= string
catalase test Correct Ans-detects presence of catalase using hydrogen peroxide, which is
broken down into H2O and O2= bubbles meaning catalos positive
oxidase test Correct Ans-detects presence of cytochrome (gram negative)--> use tooth pick
on BBC dry slide, blue= positive
kirby-bauer Correct Ans-measure susceptibility of bacteria to certain antibiotics:
lawn culture on mueller-hinton agar= measure ZOI
colilert test Correct Ans-identifies presence of coliforms/ e. coli:
yellow PSB tube= coliforms present/ beta-galactosidase (ONPG=substrate)
, BIOS 280 lab final; Questions and Answers 100% Correct
blue fluorescent= e. coli/ glutoronidase (MUG= substrate)
streak plate Correct Ans-used to isolate individual colonies, goal =10-20
spread plate Correct Ans-used when counting colonies from dilution series (used hockey
stick to spread), used to calculate the OCD, based off colony #
lawn culture Correct Ans-used for antibiotic testing, even growth across entire plate (1st
section, turn 90, turn 45)
GasPak jar Correct Ans-creates an anaerobic atmosphere: hydrogen gas combines with
oxygen (boiling) removes all oxygen
*indicators for working= indicator strip turns white or presence of oxygen
candle jar Correct Ans-creates an atmosphere with lots of CO2 and little oxygen: ideal
environment for respiratory organisms b/c some bacteria like increased CO2 and some
hemolycins don't tolerate oxygen (which is what we're growing)
