BIOS260 Chapter 9 Questions and Answers 100% Solved

BIOS260 Chapter 9 Questions and Answers 100% Solved

What substrates are used in the DNA synthesis reaction? Correct Ans-Deoxyadenosine

triphosphate


Deoxyguanosine triphosphate


Deoxycytosine triphosphate


Deoxythymidine triphosphate




List the different proteins and enzymes taking part in bacterial replication. Give the function

of each in the replication process. Correct Ans-1.Initiator proteins


bind to the origin and separates strands of DNA to initiate replication.




2. DNA helicase


unwinds the double helix by breaking the hydrogen bonding between the 2 strands at the

replication fork.


-pushes forks open to make a bubble




3. Single-stranded binding protein (SSB protein) stabilizes single-stranded DNA prior to

replication by binding to it, thus preventing the DNA from pairing with complementary

sequences.

, BIOS260 Chapter 9 Questions and Answers 100% Solved

OTHER WORDS- attach to single stranded DNA and prevent secondary structures from

forming.


-holds bubble open




4.DNA gyrase


-moves ahead of the replication fork, making and resealing breaks in the double-stranded

helical DNA to release the torque that builds up as well as a result of unwinding at the

replication fork


-untwists DNA




5. DNA primase


Specific for DNA


an RNA polymerase


Synthesizes a short RNA primer to provide a 3'-OH group for the attachment of DNA

nucleotides




6. DNA polymerase III