BCH 403 Final Exam with Questions
Solved 100% Correct | Latest Update
E1: Most nonpolar residues are located on the interior of a protein fold, but are
occasionally found on surfaces where they can mediate protein interactions. The
molecular basis of a hypothetical infectious disease is that a bacterial protein interacts
with a human protein by sharing a nonpolar surface. Your goal as a researcher is to
find a way to disrupt this interaction. Which of the following surface mutations are
most likely to disrupt the protein interactions? - Answer Leucine --> Glutamine
E1: You've discovered a new species of rodent living at a very high altitude, where the
partial pressure of oxygen is low. What would you predict regarding the oxygen binding
capacity of this new species? - Answer B and D ( it's hemoglobin will have a very low
P50 value & the primary amino acid sequence of its hemoglobin will not be identical to
that of a species living in an oxygen rich atmosphere)
E1: the following peptide is composed of which amino acid sequence
[Picture of sequence. May insert later] - Answer LKQIFKDIWTIGW
E1: The following sequence of 16 residues represents an Alpha helical stretch in an
enzyme from the bacterium that causes tuberculosis. which of the following is not
true regarding the structure of this Alpha Helix?
QFLRDTANSLLDNLIT - Answer Mutation from the phenylalanine residue to a proline
will prevent the rest of the sequence from forming an Alpha Helix.
,E1: The residue sequence of a fibrous protein is shown below. which protein does
this sequence likely correspond to?
MGISQKLYRDVNNAFQSRINYWLS - Answer fibroin
E1: Which of the following is true regarding oxygen binding to one molecule of
myoglobin? - Answer B and C (A histidine is required to hydrogen bond with the bound
oxygen thus stabilizing it. & the iron atom is located outside the plane of the heme
when oxygen is not bound)
E1: A chromatography column was used to try to purify a protein that is 10 kilodaltons
in size. Some of the illusion fractions were run on SDS page and are shown below,
with the lower numbered fractions eluting first period based only on this information,
what kind of chromatographic column was used it was the attempt successful? The
charges on the gel are shown on the left.
[May insert picture later] - Answer Nickel affinity, and it did work
E1: Your goal is to separate a mixture of the two polypeptides shown below, and
obtain purified peptide one for further study as a neurotransmitter. which purification
technique would be most effective and why?
Peptide 1: MHTRRAPLQAWKKPRADHVL
Peptide 2: MHTDEAPLQAWKDPEADHVLW - Answer Cation exchange, since peptide 1 is
more positively charged and will "stick" to that resin best.
E1: Ascorbate (Vitamin C) is an important part of any diet because: - Answer It is utilized
by the enzyme lysyl oxidase, which crosslinks lysine residues to stabilize the structure of
tropocollagen.
, E1: Why do proteins fold? - Answer A and B are correct (It is entropically favorable for
water & It is enthalpically favorable for the protein)
E1: The structural changes in hemoglobin that contribute toward its positive
cooperativity for oxygen consist of: - Answer All of the above ( movement of iron into
the plane of the heme & movement of a neighboring veiling residue due to strain
from changes in the heme & the breaking of a covalent bond with the regulatory
molecule BPG
E1: The following oxygen binding curves are derived from independent
experiments. which of the following statements is not true regarding these plots?
[Plot picture may insert later] - Answer when comparing 3 with 4 comma careforce less
likely to be bound to BPG
E1: What is the molecular basis for sickle cell anemia? - Answer A polar surface residue
in hemoglobin is mutated to valine, a nonpolar residue, causing the protein to
aggregate into fibers
E1: Which of the following tripeptides will bind to an anion exchange column at pH 7
Peptide A: Glu-Lys-Arg Peptide B: Glu-Asp-Arg - Answer Peptide B will bond
E1: Which of the following is true regarding Michaelis-Menton enzyme kinetics? -
Answer B and C are correct (the Michaelis constant (KM) is the substrate concentration
Solved 100% Correct | Latest Update
E1: Most nonpolar residues are located on the interior of a protein fold, but are
occasionally found on surfaces where they can mediate protein interactions. The
molecular basis of a hypothetical infectious disease is that a bacterial protein interacts
with a human protein by sharing a nonpolar surface. Your goal as a researcher is to
find a way to disrupt this interaction. Which of the following surface mutations are
most likely to disrupt the protein interactions? - Answer Leucine --> Glutamine
E1: You've discovered a new species of rodent living at a very high altitude, where the
partial pressure of oxygen is low. What would you predict regarding the oxygen binding
capacity of this new species? - Answer B and D ( it's hemoglobin will have a very low
P50 value & the primary amino acid sequence of its hemoglobin will not be identical to
that of a species living in an oxygen rich atmosphere)
E1: the following peptide is composed of which amino acid sequence
[Picture of sequence. May insert later] - Answer LKQIFKDIWTIGW
E1: The following sequence of 16 residues represents an Alpha helical stretch in an
enzyme from the bacterium that causes tuberculosis. which of the following is not
true regarding the structure of this Alpha Helix?
QFLRDTANSLLDNLIT - Answer Mutation from the phenylalanine residue to a proline
will prevent the rest of the sequence from forming an Alpha Helix.
,E1: The residue sequence of a fibrous protein is shown below. which protein does
this sequence likely correspond to?
MGISQKLYRDVNNAFQSRINYWLS - Answer fibroin
E1: Which of the following is true regarding oxygen binding to one molecule of
myoglobin? - Answer B and C (A histidine is required to hydrogen bond with the bound
oxygen thus stabilizing it. & the iron atom is located outside the plane of the heme
when oxygen is not bound)
E1: A chromatography column was used to try to purify a protein that is 10 kilodaltons
in size. Some of the illusion fractions were run on SDS page and are shown below,
with the lower numbered fractions eluting first period based only on this information,
what kind of chromatographic column was used it was the attempt successful? The
charges on the gel are shown on the left.
[May insert picture later] - Answer Nickel affinity, and it did work
E1: Your goal is to separate a mixture of the two polypeptides shown below, and
obtain purified peptide one for further study as a neurotransmitter. which purification
technique would be most effective and why?
Peptide 1: MHTRRAPLQAWKKPRADHVL
Peptide 2: MHTDEAPLQAWKDPEADHVLW - Answer Cation exchange, since peptide 1 is
more positively charged and will "stick" to that resin best.
E1: Ascorbate (Vitamin C) is an important part of any diet because: - Answer It is utilized
by the enzyme lysyl oxidase, which crosslinks lysine residues to stabilize the structure of
tropocollagen.
, E1: Why do proteins fold? - Answer A and B are correct (It is entropically favorable for
water & It is enthalpically favorable for the protein)
E1: The structural changes in hemoglobin that contribute toward its positive
cooperativity for oxygen consist of: - Answer All of the above ( movement of iron into
the plane of the heme & movement of a neighboring veiling residue due to strain
from changes in the heme & the breaking of a covalent bond with the regulatory
molecule BPG
E1: The following oxygen binding curves are derived from independent
experiments. which of the following statements is not true regarding these plots?
[Plot picture may insert later] - Answer when comparing 3 with 4 comma careforce less
likely to be bound to BPG
E1: What is the molecular basis for sickle cell anemia? - Answer A polar surface residue
in hemoglobin is mutated to valine, a nonpolar residue, causing the protein to
aggregate into fibers
E1: Which of the following tripeptides will bind to an anion exchange column at pH 7
Peptide A: Glu-Lys-Arg Peptide B: Glu-Asp-Arg - Answer Peptide B will bond
E1: Which of the following is true regarding Michaelis-Menton enzyme kinetics? -
Answer B and C are correct (the Michaelis constant (KM) is the substrate concentration
