BCH 361 Ch. 6 TEST QUESTIONS
WITH ANSWERS – UPDATED!!
What other aspects of a catalyzed rxn are diff from the uncatalyzed rxn?
A. the overall rxn
B. the mechanism
C. delta H rxn - Answer-B
Is this statement true for biochemical standard state, the chemical standard state, or
both?
∆G° - Answer-the chemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
the pressure is 1 atm - Answer-both
Is this statement true for biochemical standard state, chemical standard state, or both?
the temperature is 25 degrees Celcius - Answer-both
Is this statement true for biochemical standard state, chemical standard state, or both?
[H+]= 10^-7 M - Answer-biochemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
constant value for [Mg2+] - Answer-biochemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
the initial concentration of reactants and products is 1 M - Answer-both
Is this statement true for biochemical standard state, chemical standard state, or both?
pH 0 - Answer-chemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
∆G°' or ∆G'° - Answer-biochemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
the change in [H2O] is assumed to be insignificant - Answer-biochemical standard state
Is chymotrypsin specific of nonspecific? - Answer-specific
Describe how chymotrypsin works - Answer-splits the peptide bonds only on the
carboxyl side of large hydrophobic residues (W,Y,F,M)
Is Thrombin specific or nonspecific? - Answer-very specific
Describe how thrombin works - Answer-catalyzes the hydrolysis of arginine-glycine
bonds in particular peptide sequence only
Where is thrombin found? - Answer-in blood clotting
, Name the 6 major types of enzymes - Answer-oxidoreductases, transferases,
hydrolases, lyases, isomerases, ligases
Oxidoreductases - Answer-catalyze oxidation-reduction rxns
examples of oxidoreductases - Answer-dehydrogenase, transfer of elections, hydride
example of transferases - Answer-kinases
transferases - Answer-move functional groups btw molecules
hydrolases - Answer-cleave bonds w/ the addition of water
examples of hydrolases - Answer-nucleases, peptidases, proteases, lipases, amylase
lyases - Answer-remove atoms to form double bonds or add atoms to double bonds
examples of lyases - Answer-enolase, fumarase
isomerases - Answer-rearrange atoms w/in a molecule
examples of isomerases - Answer-triose phosphate isomerase (reorganization of
carbons), mutase (move functional group intramolecularly)
ligases - Answer-join 2 molecules at the expense of ATP
examples of ligases - Answer-DNA ligase, DNA polymerases, DNA polyerases
WITH ANSWERS – UPDATED!!
What other aspects of a catalyzed rxn are diff from the uncatalyzed rxn?
A. the overall rxn
B. the mechanism
C. delta H rxn - Answer-B
Is this statement true for biochemical standard state, the chemical standard state, or
both?
∆G° - Answer-the chemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
the pressure is 1 atm - Answer-both
Is this statement true for biochemical standard state, chemical standard state, or both?
the temperature is 25 degrees Celcius - Answer-both
Is this statement true for biochemical standard state, chemical standard state, or both?
[H+]= 10^-7 M - Answer-biochemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
constant value for [Mg2+] - Answer-biochemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
the initial concentration of reactants and products is 1 M - Answer-both
Is this statement true for biochemical standard state, chemical standard state, or both?
pH 0 - Answer-chemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
∆G°' or ∆G'° - Answer-biochemical standard state
Is this statement true for biochemical standard state, chemical standard state, or both?
the change in [H2O] is assumed to be insignificant - Answer-biochemical standard state
Is chymotrypsin specific of nonspecific? - Answer-specific
Describe how chymotrypsin works - Answer-splits the peptide bonds only on the
carboxyl side of large hydrophobic residues (W,Y,F,M)
Is Thrombin specific or nonspecific? - Answer-very specific
Describe how thrombin works - Answer-catalyzes the hydrolysis of arginine-glycine
bonds in particular peptide sequence only
Where is thrombin found? - Answer-in blood clotting
, Name the 6 major types of enzymes - Answer-oxidoreductases, transferases,
hydrolases, lyases, isomerases, ligases
Oxidoreductases - Answer-catalyze oxidation-reduction rxns
examples of oxidoreductases - Answer-dehydrogenase, transfer of elections, hydride
example of transferases - Answer-kinases
transferases - Answer-move functional groups btw molecules
hydrolases - Answer-cleave bonds w/ the addition of water
examples of hydrolases - Answer-nucleases, peptidases, proteases, lipases, amylase
lyases - Answer-remove atoms to form double bonds or add atoms to double bonds
examples of lyases - Answer-enolase, fumarase
isomerases - Answer-rearrange atoms w/in a molecule
examples of isomerases - Answer-triose phosphate isomerase (reorganization of
carbons), mutase (move functional group intramolecularly)
ligases - Answer-join 2 molecules at the expense of ATP
examples of ligases - Answer-DNA ligase, DNA polymerases, DNA polyerases
