BCH 403 FINAL TEST QUESTIONS
WITH VERIFIED ANSWERS
Which one of the following proteins catalyzes the 5' --> 5' bond when adding the 5' cap
to mRNA - Answer-RNA guanylyltransferase
which one of the following mRNA sequences contain an arginine residue as the 4th
amino acid in the reading frame 3? A codon table is located on the last page of this
exam booklet - Answer-5' UUUGACCGACCAGAACCC 3'
Which one of the following is not involved in translation initiation in prokaryotes? -
Answer-Methionyl t-RNA
Which of the following post-translational modifications is most common in humans -
Answer-Phosphorylation
Which one fo the following will form a complex with small RNA to form the RNA
silencing inducing complex - Answer-Argonaut
Common genetic variances (i.e. genetic variances that occur within at least one percent
of the population) are called - Answer-Polymorphisms
Which one of the following is best suited to identify a large chromosomal disorder (i.e. a
disorder that changes the number or appearance of a chromosome)? - Answer-
Cytogenic testing
According to the mutator hypothesis, which one of the following is responsible for
bypassing the barriers that would normally suppress tumor development? - Answer-
Driver mutations
Sporadic cancers - Answer-are a result of mutations and epigenetic changes acquired
from environmental factors or metabolic accidents
Which one of the following is NOT a post-transcriptional modification - Answer-
Riboswitch
Which one of the following describes the splicesome - Answer-SnRNPs complexed with
mRNA
Aminoacyl-tRNA synthetases catalyze the covalent interaction between tRNA and an
amino acid. Which one of the following molecules is covalently bonded to AMP during
this process - Answer-amino acid
Which one of the following codons changes would result in silent mutation? A codon
table is located on the last page of this exam booklet - Answer-AGA-->CGA
Which one of the following ribosomal subunits are responsible for peptidyltransferases
activity in eukaryotes - Answer-28S rRNA
, Which one of the following best describes translocation during protein synthesis -
Answer-EF2 moves the ribosome one codon towards 3' of the mRNA template
TOM and TIM barrels are involved in trafficking proteins to which organelle - Answer-
Mitochondria
Which of the following techniques should be used when sequencing an entire human
genome - Answer-DNA-seq
Which one of the following is used in CRISPR to guide the complex to the DNA
sequence - Answer-gRNA
which one of the following is NOT a type of oncogene - Answer-Growth suppressors
We can often predict protein encoding genes by looking for several elements within the
DNA sequence. Which one of the following is NOT used in gene predictions for
eukaryotes - Answer-Shine-delgarno sequence
The composition of a bilayer has been modified to include 10% cholesterol. Which of
the following is true regarding the new properties of the bilayer - Answer-The Tm of the
bilayer has not changed
AND
The rigidity of the cholesterol broadens the transition temperature of the bilayer
A integral membrane protein that is expected to b eon the plasma membrane is instead
found on the cytoplasm. Which of the following would explain this result - Answer-The
SRP is not able to recognize the N-terminal signal sequence
What might occur if the Zn2+ prosthetic group was no longe present in the enzyme
Carboxypeptidase A during a reaction? - Answer-the delta G double dagger would
increase
Anabolic pathways require an energy input to synthesize mroe complex molecules form
less complex molecules - Answer-True
Phosphoenolpyruvate carboxykinase is found within the mitochondria - Answer-false
Fructose-2,6-bisphosphate inactviates fructose-1,6-bisphophatase by directly binding to
the active site of the enzyme (i.e. its a competitive inhibitor) - Answer-false
Malate/Aspartate shuttle is used by the liver to transport two electrons from cytosolic
NADH onto mitochondrial NADH - Answer-True
Succinate dehydrogenase complex draws two electrons from succinate and donates
them to coenzyme Q - Answer-True
Hexokinase phosphorylates glucose so that glucose can not be transported out of the
cell - Answer-true
During glycogenolysis, debranching enzymes can synthesize alpha (1-->4) linkages -
WITH VERIFIED ANSWERS
Which one of the following proteins catalyzes the 5' --> 5' bond when adding the 5' cap
to mRNA - Answer-RNA guanylyltransferase
which one of the following mRNA sequences contain an arginine residue as the 4th
amino acid in the reading frame 3? A codon table is located on the last page of this
exam booklet - Answer-5' UUUGACCGACCAGAACCC 3'
Which one of the following is not involved in translation initiation in prokaryotes? -
Answer-Methionyl t-RNA
Which of the following post-translational modifications is most common in humans -
Answer-Phosphorylation
Which one fo the following will form a complex with small RNA to form the RNA
silencing inducing complex - Answer-Argonaut
Common genetic variances (i.e. genetic variances that occur within at least one percent
of the population) are called - Answer-Polymorphisms
Which one of the following is best suited to identify a large chromosomal disorder (i.e. a
disorder that changes the number or appearance of a chromosome)? - Answer-
Cytogenic testing
According to the mutator hypothesis, which one of the following is responsible for
bypassing the barriers that would normally suppress tumor development? - Answer-
Driver mutations
Sporadic cancers - Answer-are a result of mutations and epigenetic changes acquired
from environmental factors or metabolic accidents
Which one of the following is NOT a post-transcriptional modification - Answer-
Riboswitch
Which one of the following describes the splicesome - Answer-SnRNPs complexed with
mRNA
Aminoacyl-tRNA synthetases catalyze the covalent interaction between tRNA and an
amino acid. Which one of the following molecules is covalently bonded to AMP during
this process - Answer-amino acid
Which one of the following codons changes would result in silent mutation? A codon
table is located on the last page of this exam booklet - Answer-AGA-->CGA
Which one of the following ribosomal subunits are responsible for peptidyltransferases
activity in eukaryotes - Answer-28S rRNA
, Which one of the following best describes translocation during protein synthesis -
Answer-EF2 moves the ribosome one codon towards 3' of the mRNA template
TOM and TIM barrels are involved in trafficking proteins to which organelle - Answer-
Mitochondria
Which of the following techniques should be used when sequencing an entire human
genome - Answer-DNA-seq
Which one of the following is used in CRISPR to guide the complex to the DNA
sequence - Answer-gRNA
which one of the following is NOT a type of oncogene - Answer-Growth suppressors
We can often predict protein encoding genes by looking for several elements within the
DNA sequence. Which one of the following is NOT used in gene predictions for
eukaryotes - Answer-Shine-delgarno sequence
The composition of a bilayer has been modified to include 10% cholesterol. Which of
the following is true regarding the new properties of the bilayer - Answer-The Tm of the
bilayer has not changed
AND
The rigidity of the cholesterol broadens the transition temperature of the bilayer
A integral membrane protein that is expected to b eon the plasma membrane is instead
found on the cytoplasm. Which of the following would explain this result - Answer-The
SRP is not able to recognize the N-terminal signal sequence
What might occur if the Zn2+ prosthetic group was no longe present in the enzyme
Carboxypeptidase A during a reaction? - Answer-the delta G double dagger would
increase
Anabolic pathways require an energy input to synthesize mroe complex molecules form
less complex molecules - Answer-True
Phosphoenolpyruvate carboxykinase is found within the mitochondria - Answer-false
Fructose-2,6-bisphosphate inactviates fructose-1,6-bisphophatase by directly binding to
the active site of the enzyme (i.e. its a competitive inhibitor) - Answer-false
Malate/Aspartate shuttle is used by the liver to transport two electrons from cytosolic
NADH onto mitochondrial NADH - Answer-True
Succinate dehydrogenase complex draws two electrons from succinate and donates
them to coenzyme Q - Answer-True
Hexokinase phosphorylates glucose so that glucose can not be transported out of the
cell - Answer-true
During glycogenolysis, debranching enzymes can synthesize alpha (1-->4) linkages -
