MLT 106 Unit 8 Test Questions and
Answers All Correct
What occurs in flow cell cytometry? - Answer-Stained cells pass through a laser; dye
reacts and fluoresces
What is used to enhance and stabilize precipitates in nephelometry? - Answer-PEG
What does nephelometry measure? - Answer-Complement components
Immune complexes
Presence of antibodies
(measures patient proteins)
What is the application of flow cell cytometry? - Answer-Cell identification
Cell sorting
What are the phases of PCR? How many cycles are performed? - Answer-Denature,
primer annealing, extension 30
What is the gold standard, and its most frequently used method? Briefly explain the
method - Answer-DNA sequencing
Sanger method- produces complementary segments to the DNA being sequenced
What is the importance of the dot blot and reverse dot blot? - Answer-Detects disorders
in which the DNA sequence of the mutated region has been identified
Sickle cell anemia and cystic fibrosis distinguishes between homozygous and
heterozygous state
What is reverse transcriptase? - Answer-An enzyme the transcribes single-stranded
RNA into DNA
What is the nucleic-acid sequence-based amplification target? - Answer-RNA
What is reverse transcriptase PCR used to detect? - Answer-HIV and HEP C
What is nucleic-acid sequence-based amplification used to detect? - Answer-HIV and
CMV
What does the southern blot measure? - Answer-Single stranded DNA
What does the northern blot measure? - Answer-mRNA
Answers All Correct
What occurs in flow cell cytometry? - Answer-Stained cells pass through a laser; dye
reacts and fluoresces
What is used to enhance and stabilize precipitates in nephelometry? - Answer-PEG
What does nephelometry measure? - Answer-Complement components
Immune complexes
Presence of antibodies
(measures patient proteins)
What is the application of flow cell cytometry? - Answer-Cell identification
Cell sorting
What are the phases of PCR? How many cycles are performed? - Answer-Denature,
primer annealing, extension 30
What is the gold standard, and its most frequently used method? Briefly explain the
method - Answer-DNA sequencing
Sanger method- produces complementary segments to the DNA being sequenced
What is the importance of the dot blot and reverse dot blot? - Answer-Detects disorders
in which the DNA sequence of the mutated region has been identified
Sickle cell anemia and cystic fibrosis distinguishes between homozygous and
heterozygous state
What is reverse transcriptase? - Answer-An enzyme the transcribes single-stranded
RNA into DNA
What is the nucleic-acid sequence-based amplification target? - Answer-RNA
What is reverse transcriptase PCR used to detect? - Answer-HIV and HEP C
What is nucleic-acid sequence-based amplification used to detect? - Answer-HIV and
CMV
What does the southern blot measure? - Answer-Single stranded DNA
What does the northern blot measure? - Answer-mRNA
