MLT 106 Unit 4 Test Questions and
Answers All Correct
What is exclusion? - Answer-process in which antibodies that could not be responsible
for reactivity are excluded
What method involves comparing antigen-positive cells to pattern of reactivity? -
Answer-Inclusion
What is the process in which the RBC surface is modified by removing sialic acid
residues and denaturing or removing glycoproteins? - Answer-enzyme treatment
What is the term defined as removing adsorbed material from an adsorbent by means of
a solvent? - Answer-Elution
What blood group system is associated with malaria (plasmodium vivax)? - Answer-
Duffy
True or false? Lewis antigens are intrinsic to the RBC and are glycosphingolipids. -
Answer-false; made in tissue, found in plasma
Group B individuals who phenotype lele Sese will secrete which antigens? - Answer-B,
H
Your Se gene modifies ___ chains wjile the H gene modifies ___ chains. - Answer-Type
1
Type 2
Lewis antigens have receptors that interact with what microorganism? - Answer-
helicobactor pylori
Which antigens are located on GPA? - Answer-M and N
Which of the following are naturally occurring: anti-M and anti-N or anti-S, -s, -U. -
Answer-S s U
What is the Donath-Landsteiner antibody? - Answer-anti-P
biphasic hemolysin
What are all the Kidd antibodies? - Answer-Jka, Jkb, Jk3
Kell null phenotype produces which clinically significant antibody? - Answer-anti-Ku
, Which enzymes do not destroy K antigens? - Answer-Ficin and papain
The Jk(a-b-) phenotype has been identifed in what populations? - Answer-chinese and
polynesian
How can you test for Jk(a-b-)? - Answer-no lysis in 2M urea
What are 2 methods that cause expression of Jk(a-b-)? - Answer-genetic mutations
suppressed by In(Jk)
What is the only insignificant P antibody? - Answer-P1
Which AHG is produced using hybridoma technology? - Answer-Monoclonal
To minimize in vitro red cell sensitization, how should you collect specimens? - Answer-
EDTA and room temp
RESt possesses what structures? - Answer-I, H, and IH
What is the technique in which one excludes antibodies that could not be responsible
for reactivity seen? What is advised? - Answer-rule out technique
only for homozygous expression
What type of manufactured cells are used in panel evaluation? What phases are
tested? - Answer-O cells in 3 phases of testing (IS, 37, AHG)
What is RESt? - Answer-rabbit erythrocyte stroma; removes insignificant antibodies
If phenotyping is difficult, what may be performed? - Answer-homologous adsorption
What is AHG? What is the difference between mono- and polyspecific? - Answer-
antihuman globulin; green bottle reagent
mono has specific complement or anti-IgG
poly contains several
What technology produces monoclonal antibodies? - Answer-hybridoma - mouse spleen
lymphocyte with myeloma
What produces polyclonal antibodies? - Answer-rabbits; inject animals and harvest
antibodies
What is the difference between IAT and DAT? - Answer-in vitro - in a tube
in vivo - in the body (no incubation phase)
Why would you run a DAT? - Answer-transfusion within 2 weeks
Answers All Correct
What is exclusion? - Answer-process in which antibodies that could not be responsible
for reactivity are excluded
What method involves comparing antigen-positive cells to pattern of reactivity? -
Answer-Inclusion
What is the process in which the RBC surface is modified by removing sialic acid
residues and denaturing or removing glycoproteins? - Answer-enzyme treatment
What is the term defined as removing adsorbed material from an adsorbent by means of
a solvent? - Answer-Elution
What blood group system is associated with malaria (plasmodium vivax)? - Answer-
Duffy
True or false? Lewis antigens are intrinsic to the RBC and are glycosphingolipids. -
Answer-false; made in tissue, found in plasma
Group B individuals who phenotype lele Sese will secrete which antigens? - Answer-B,
H
Your Se gene modifies ___ chains wjile the H gene modifies ___ chains. - Answer-Type
1
Type 2
Lewis antigens have receptors that interact with what microorganism? - Answer-
helicobactor pylori
Which antigens are located on GPA? - Answer-M and N
Which of the following are naturally occurring: anti-M and anti-N or anti-S, -s, -U. -
Answer-S s U
What is the Donath-Landsteiner antibody? - Answer-anti-P
biphasic hemolysin
What are all the Kidd antibodies? - Answer-Jka, Jkb, Jk3
Kell null phenotype produces which clinically significant antibody? - Answer-anti-Ku
, Which enzymes do not destroy K antigens? - Answer-Ficin and papain
The Jk(a-b-) phenotype has been identifed in what populations? - Answer-chinese and
polynesian
How can you test for Jk(a-b-)? - Answer-no lysis in 2M urea
What are 2 methods that cause expression of Jk(a-b-)? - Answer-genetic mutations
suppressed by In(Jk)
What is the only insignificant P antibody? - Answer-P1
Which AHG is produced using hybridoma technology? - Answer-Monoclonal
To minimize in vitro red cell sensitization, how should you collect specimens? - Answer-
EDTA and room temp
RESt possesses what structures? - Answer-I, H, and IH
What is the technique in which one excludes antibodies that could not be responsible
for reactivity seen? What is advised? - Answer-rule out technique
only for homozygous expression
What type of manufactured cells are used in panel evaluation? What phases are
tested? - Answer-O cells in 3 phases of testing (IS, 37, AHG)
What is RESt? - Answer-rabbit erythrocyte stroma; removes insignificant antibodies
If phenotyping is difficult, what may be performed? - Answer-homologous adsorption
What is AHG? What is the difference between mono- and polyspecific? - Answer-
antihuman globulin; green bottle reagent
mono has specific complement or anti-IgG
poly contains several
What technology produces monoclonal antibodies? - Answer-hybridoma - mouse spleen
lymphocyte with myeloma
What produces polyclonal antibodies? - Answer-rabbits; inject animals and harvest
antibodies
What is the difference between IAT and DAT? - Answer-in vitro - in a tube
in vivo - in the body (no incubation phase)
Why would you run a DAT? - Answer-transfusion within 2 weeks
