ASCP MLT Chemistry Exam Questions
with Answers
What causes cloudiness in a freshly voided urine? - Answer-white blood cells
What is a refractometer used to measure? - Answer-specific gravity
Which systems may be used to determine the specific gravity urine? - Answer-
refractometer, harmonic oscillation, reagent test strip
What is the best single indicator of renal disease? - Answer-proteinuria
What applies to the dipstick test for urine protein, based on the protein error of
indicators? - Answer-Most of the indicator is in the non-ionized form. The protein will
combine with the non-ionized form of the indicator. Protein combined with the indicator
changes the ratio of ionized and non-ionized forms
What applies to urine protein estimation by the dipstick method? - Answer-Highly
alkaline buffered urine can cause a false positive test. The principle of the test is the
protein error of indicators. The test is more sensitive to albumin than to globulin
What is true of the detection of urinary glucose? - Answer-any reducing substance can
give a false positive reaction with the copper reduction method for glucose
The urine dipstick test for occult blood depends on which fact? - Answer-Hemoglobin
can act as a peroxidase
To catheterized specimen, in most instances, which specimen is preferred? - Answer-a
clean catch urine
the presence of bacteria in uncentrifuged specimen of urine collected under controlled
conditions is associated? - Answer-with a significant colony count
The presence of bacteriuria can be recognized by? - Answer-a positive nitrite result
Red blood cells in the urine may be confused with what? - Answer-yeast cells, oil
droplets, calcium oxalate crystals
What does NOT apply to white blood cells in the urine sediment? - Answer-White blood
cells in urine appear spherical and granular
White blood cells are frequently seen in patients with urinary tract infections. What type
of white blood cell is seen most frequently in the urine sediment? - Answer-neutrophil
, A glitter cell is a term used to describe a specific type of what? - Answer-leukocyte
Vaginal contamination may be responsible for the appearance of which element in the
urine from a female patient? - Answer-squamous epithelial cells
The matrix of urinary casts has be identified as what protein? - Answer-Tamm-Horsfall
mucoprotein
Casts formed primarily in what portion of the kidney? - Answer-distal convoluted tubule
What is true of casts in urine? - Answer-they tend to accumulate near the edges of the
coverslip
Which cast may appear in urine in the absence of renal disease? - Answer-hyaline
Which element in proteins differentiates substance from carbohydrates and lipids? -
Answer-nitrogen
What proteins is soluble in water? - Answer-albumin
How are amino acids joined together to form a chain like structure? - Answer-peptide
bonding
Proteins may become denatured when subjected to? - Answer-mechanical agitation,
heat, chemical treatments
What does denaturation of proteins refer to? - Answer-alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - Answer-cystinuria
How many immunoglobulin classes are currently recognized? - Answer-five
which reagent is employed in the serum protein determination? - Answer-biuret
What should hemolyzed serum not be used for total protein analysis? - Answer-
hemoglobin is absorbed at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - Answer-is relatively free from interference by
lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
Answer-there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - Answer-albumin
with Answers
What causes cloudiness in a freshly voided urine? - Answer-white blood cells
What is a refractometer used to measure? - Answer-specific gravity
Which systems may be used to determine the specific gravity urine? - Answer-
refractometer, harmonic oscillation, reagent test strip
What is the best single indicator of renal disease? - Answer-proteinuria
What applies to the dipstick test for urine protein, based on the protein error of
indicators? - Answer-Most of the indicator is in the non-ionized form. The protein will
combine with the non-ionized form of the indicator. Protein combined with the indicator
changes the ratio of ionized and non-ionized forms
What applies to urine protein estimation by the dipstick method? - Answer-Highly
alkaline buffered urine can cause a false positive test. The principle of the test is the
protein error of indicators. The test is more sensitive to albumin than to globulin
What is true of the detection of urinary glucose? - Answer-any reducing substance can
give a false positive reaction with the copper reduction method for glucose
The urine dipstick test for occult blood depends on which fact? - Answer-Hemoglobin
can act as a peroxidase
To catheterized specimen, in most instances, which specimen is preferred? - Answer-a
clean catch urine
the presence of bacteria in uncentrifuged specimen of urine collected under controlled
conditions is associated? - Answer-with a significant colony count
The presence of bacteriuria can be recognized by? - Answer-a positive nitrite result
Red blood cells in the urine may be confused with what? - Answer-yeast cells, oil
droplets, calcium oxalate crystals
What does NOT apply to white blood cells in the urine sediment? - Answer-White blood
cells in urine appear spherical and granular
White blood cells are frequently seen in patients with urinary tract infections. What type
of white blood cell is seen most frequently in the urine sediment? - Answer-neutrophil
, A glitter cell is a term used to describe a specific type of what? - Answer-leukocyte
Vaginal contamination may be responsible for the appearance of which element in the
urine from a female patient? - Answer-squamous epithelial cells
The matrix of urinary casts has be identified as what protein? - Answer-Tamm-Horsfall
mucoprotein
Casts formed primarily in what portion of the kidney? - Answer-distal convoluted tubule
What is true of casts in urine? - Answer-they tend to accumulate near the edges of the
coverslip
Which cast may appear in urine in the absence of renal disease? - Answer-hyaline
Which element in proteins differentiates substance from carbohydrates and lipids? -
Answer-nitrogen
What proteins is soluble in water? - Answer-albumin
How are amino acids joined together to form a chain like structure? - Answer-peptide
bonding
Proteins may become denatured when subjected to? - Answer-mechanical agitation,
heat, chemical treatments
What does denaturation of proteins refer to? - Answer-alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - Answer-cystinuria
How many immunoglobulin classes are currently recognized? - Answer-five
which reagent is employed in the serum protein determination? - Answer-biuret
What should hemolyzed serum not be used for total protein analysis? - Answer-
hemoglobin is absorbed at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - Answer-is relatively free from interference by
lipids and hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
Answer-there is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - Answer-albumin
