resistance to
> MRNA is easier to find desired gene. reverse gene cloning ↓
A
& J
asmid I
plrecognition
desire set
g
no introns transcriptase makes (copies) CDNA
. Restriction Invitro (PCR) in vivo
·
endonucleases cut up CDNA
making sticky ends
. separate DNA95% plasmids (vector)
g
resistance
fragments to plasmids
. removed with To test transfer,
DNA
ligase joins up annealing primers gene a to B
%
55 C restriction will be resistant to
B
(sticking) enzyme
gene machine shows base sequence based ·
tag polymerase ·
join Vector and DNA and not to A
*
<
g
on a protein PCR
C an
. be used to amplify adds nucleotides 72%
fragment with DNA ligase
Interrupted
↑ >
-
fast ·
Recombinant DNA introduced
to bacterium
Selectable
markers
genetic
a
universal
gene therapy
>
-
non-overlaping
& degenerate
Tag
polymerase
↓
will not
denature
at 95%
-
genetic fingerprinting
extraction from sample
digestion < restriction endonucleases
separating -
gel electrophoresis
*
Annealing
separation n transfered to nylon membrane
risk of contaminated DNA
amplifying a hybridisation< radioactive probes attach
·
development placed onto
xray film
> MRNA is easier to find desired gene. reverse gene cloning ↓
A
& J
asmid I
plrecognition
desire set
g
no introns transcriptase makes (copies) CDNA
. Restriction Invitro (PCR) in vivo
·
endonucleases cut up CDNA
making sticky ends
. separate DNA95% plasmids (vector)
g
resistance
fragments to plasmids
. removed with To test transfer,
DNA
ligase joins up annealing primers gene a to B
%
55 C restriction will be resistant to
B
(sticking) enzyme
gene machine shows base sequence based ·
tag polymerase ·
join Vector and DNA and not to A
*
<
g
on a protein PCR
C an
. be used to amplify adds nucleotides 72%
fragment with DNA ligase
Interrupted
↑ >
-
fast ·
Recombinant DNA introduced
to bacterium
Selectable
markers
genetic
a
universal
gene therapy
>
-
non-overlaping
& degenerate
Tag
polymerase
↓
will not
denature
at 95%
-
genetic fingerprinting
extraction from sample
digestion < restriction endonucleases
separating -
gel electrophoresis
*
Annealing
separation n transfered to nylon membrane
risk of contaminated DNA
amplifying a hybridisation< radioactive probes attach
·
development placed onto
xray film
